Combined assembloid modeling and 3D whole-organ mapping captures the microanatomy and function of the human fallopian tube.

Humans Female Fallopian Tubes / anatomy & histology Imaging, Three-Dimensional / methods Proteomics / methods Models, Biological Single-Cell Analysis / methods

Journal

Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440

Informations de publication

Date de publication:
27 Sep 2024
Historique:
medline: 27 9 2024
pubmed: 27 9 2024
entrez: 27 9 2024
Statut: ppublish

Résumé

The fallopian tubes play key roles in processes from pregnancy to ovarian cancer where three-dimensional (3D) cellular and extracellular interactions are important to their pathophysiology. Here, we develop a 3D multicompartment assembloid model of the fallopian tube that molecularly, functionally, and architecturally resembles the organ. Global label-free proteomics, innovative assays capturing physiological functions of the fallopian tube (i.e., oocyte transport), and whole-organ single-cell resolution mapping are used to validate these assembloids through a multifaceted platform with direct comparisons to fallopian tube tissue. These techniques converge at a unique combination of assembloid parameters with the highest similarity to the reference fallopian tube. This work establishes (i) an optimized model of the human fallopian tubes for in vitro studies of their pathophysiology and (ii) an iterative platform for customized 3D in vitro models of human organs that are molecularly, functionally, and microanatomically accurate by combining tunable assembloid and tissue mapping methods.

Identifiants

DOI: 10.1126/sciadv.adp6285 PMID: 39331707
pubmed: 39331707
doi: 10.1126/sciadv.adp6285
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

eadp6285

Auteurs

Ashleigh J Crawford (AJ)

Johns Hopkins Institute for Nanobiotechnology, The Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center, The Johns Hopkins University, Baltimore, MD 21218, USA.
ORCID: 0000-0001-9048-0113

André Forjaz (A)

Johns Hopkins Institute for Nanobiotechnology, The Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center, The Johns Hopkins University, Baltimore, MD 21218, USA.
ORCID: 0009-0002-5115-2293

Joanna Bons (J)

Buck Institute for Research on Aging, Novato, CA 94945, USA.
ORCID: 0000-0002-1110-4193

Isha Bhorkar (I)

Johns Hopkins Institute for Nanobiotechnology, The Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA.

Triya Roy (T)

Johns Hopkins Institute for Nanobiotechnology, The Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center, The Johns Hopkins University, Baltimore, MD 21218, USA.

David Schell (D)

Johns Hopkins Institute for Nanobiotechnology, The Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center, The Johns Hopkins University, Baltimore, MD 21218, USA.

Vasco Queiroga (V)

Johns Hopkins Institute for Nanobiotechnology, The Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center, The Johns Hopkins University, Baltimore, MD 21218, USA.
ORCID: 0009-0004-7812-0472

Kehan Ren (K)

Johns Hopkins Institute for Nanobiotechnology, The Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA.

Donald Kramer (D)

Johns Hopkins Institute for Nanobiotechnology, The Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Biotechnology, Johns Hopkins Advanced Academic Programs, The Johns Hopkins University, Baltimore, MD 21218, USA.
ORCID: 0009-0004-3381-119X

Wilson Huang (W)

Johns Hopkins Institute for Nanobiotechnology, The Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA.

Gabriella C Russo (GC)

Johns Hopkins Institute for Nanobiotechnology, The Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center, The Johns Hopkins University, Baltimore, MD 21218, USA.

Meng-Horng Lee (MH)

Johns Hopkins Institute for Nanobiotechnology, The Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center, The Johns Hopkins University, Baltimore, MD 21218, USA.

Pei-Hsun Wu (PH)

Johns Hopkins Institute for Nanobiotechnology, The Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center, The Johns Hopkins University, Baltimore, MD 21218, USA.
ORCID: 0000-0002-7371-2960

Ie-Ming Shih (IM)

Department of Gynecology and Obstetrics, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Tian-Li Wang (TL)

Department of Gynecology and Obstetrics, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
ORCID: 0000-0002-7082-449X

Mark A Atkinson (MA)

Departments of Pathology, Immunology, and Laboratory Medicine, College of Medicine, University of Florida Diabetes Institute, Gainesville, FL 32610, USA.
Departments of Pediatrics, College of Medicine, University of Florida Diabetes Institute, Gainesville, FL 32610, USA.
ORCID: 0000-0001-8489-4782

Birgit Schilling (B)

Buck Institute for Research on Aging, Novato, CA 94945, USA.
ORCID: 0000-0001-9907-2749

Ashley L Kiemen (AL)

Johns Hopkins Institute for Nanobiotechnology, The Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center, The Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Department of Functional Anatomy and Evolution, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
ORCID: 0000-0002-6281-2616

Denis Wirtz (D)

Johns Hopkins Institute for Nanobiotechnology, The Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences-Oncology Center, The Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA.
Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
ORCID: 0000-0001-6147-3045

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Combined assembloid modeling and 3D...
questionsmedicales.fr › Structures anatomiques de l'animal › Oviductes › Trompes utérines › Combined assembloid modeling and 3D...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Traitement d'image par ordinateur › Imagerie tridimensionnelle › Combined assembloid modeling and 3D...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Protéomique › Combined assembloid modeling and 3D...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles biologiques › Combined assembloid modeling and 3D...
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Analyse sur cellule unique › Combined assembloid modeling and 3D...