The Kidney Precision Medicine Project and Single-Cell Biology of the Injured Proximal Tubule.

Single-cell RNA sequencing (scRNA-seq) has led to major advances in our understanding of proximal tubule subtypes in health and disease. The proximal tubule serves essential functions in overall homeostasis, but pathologic or physiologic perturbations can affect its transcriptomic signature and corresponding tasks. These alterations in proximal tubular cells are often described within a scRNA-seq atlas as cell states, which are pathophysiologic subclassifications based on molecular and morphological changes in a cell's response to that injury compared to its native state. This review describes the major cell states defined in the Kidney Precision Medicine Project's (KPMP) scRNA-seq atlas. The review then identifies the overlap between KPMP and other seminal works which may use different nomenclature or cluster proximal tubule cells at different resolutions to define cell state subtypes. The goal is for the reader to understand the key transcriptomic markers of important cellular injury and regeneration processes across this highly dynamic and evolving field.

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Copyright © 2024. Published by Elsevier Inc.