G protein coupled receptor transcripts in human immune cells and platelets.
Journal
Scientific data
ISSN: 2052-4463
Titre abrégé: Sci Data
Pays: England
ID NLM: 101640192
Informations de publication
Date de publication:
27 Sep 2024
27 Sep 2024
Historique:
received:
24
04
2024
accepted:
13
09
2024
medline:
28
9
2024
pubmed:
28
9
2024
entrez:
27
9
2024
Statut:
epublish
Résumé
G-protein coupled receptors (GPCRs) are encoded by nonabundant mRNAs, and it is difficult to detect them reliably with the highly parallel methods that are in general use. Because of this, we developed and validated a sensitive, specific, semi-quantitative method for detecting these transcripts. We have used the method to profile GPCR transcripts in white blood cells (WBCs)-B, CD4, CD8, NK, and dendritic cells; monocytes, and macrophage-like monocytes treated with granulocyte-macrophage colony-stimulating factor-as well as platelets. On average, the white cells studied expressed 160 receptor mRNAs (range, 123-206). Platelets made 69. Some, but far from all, of the receptors we found have been detected earlier. We believe our data should stimulate studies of receptor function and contribute to drug development.
Identifiants
pubmed: 39333175
doi: 10.1038/s41597-024-03880-2
pii: 10.1038/s41597-024-03880-2
doi:
Substances chimiques
Receptors, G-Protein-Coupled
0
RNA, Messenger
0
Types de publication
Journal Article
Dataset
Langues
eng
Sous-ensembles de citation
IM
Pagination
1040Informations de copyright
© 2024. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.
Références
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