Microenvironment-responsive, multimodulated herbal polysaccharide hydrogel for diabetic foot ulcer healing.
Animals
Wound Healing
/ drug effects
Polysaccharides
/ pharmacology
Mice
Diabetic Foot
/ drug therapy
Hydrogels
/ chemistry
Berberine
/ pharmacology
Staphylococcus aureus
/ drug effects
Anti-Bacterial Agents
/ pharmacology
Escherichia coli
/ drug effects
Male
Humans
Anti-Inflammatory Agents
/ pharmacology
Antioxidants
/ pharmacology
Borates
/ pharmacology
Bletilla striata polysaccharide
Berberine
Diabetic ulcers
Hydrogel
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
27 Sep 2024
27 Sep 2024
Historique:
received:
17
05
2024
accepted:
12
09
2024
medline:
28
9
2024
pubmed:
28
9
2024
entrez:
27
9
2024
Statut:
epublish
Résumé
Diabetic ulcers (DUs) usually suffer from severe infections, persistent inflammation, and excessive oxidative stress during the healing process, which led to the microenvironmental alternation and severely impede DU healing, resulting in a delayed wound healing. Therefore, it is particularly important to develop a medical dressing that can address these problems simultaneously. To this end, self-healing composite hydrogels were prepared in this study utilizing Bletilla striata polysaccharide (BSP) and Berberine (BER) with borax via borate ester bond. The chemical and mechanical properties of the BSP/BER hydrogels were characterized, and their wound healing performance was investigated in vivo and in vitro. The results showed that the BSP/BER hydrogel significantly accelerated wound healing in DU mice with the healing rate of 94.90 ± 1.81% on the 14th day by using BSP/BER5, and this outstanding performance was achieved by the multi-targeted biological functions of antibacterial, anti-inflammatory and antioxidant, which provided favorable microenvironment for orderly recovery of the wound. Aside from exhibiting the antibacterial rate of over 90% against both Escherichia coli and Staphylococcus aureus, the BSP/BER5 hydrogel could significantly reduce NO levels 4.544 ± 0.32 µmol/L to exert its anti-inflammatory effects. Additionally, it demonstrated a hemolysis rate and promotes cell migration capabilities at (34.92 ± 1.66%). With the above features, the developed BSP/BER hydrogel in this study could be the potential dressing for clinical treatment of DU wound.
Identifiants
pubmed: 39333183
doi: 10.1038/s41598-024-72972-1
pii: 10.1038/s41598-024-72972-1
doi:
Substances chimiques
Polysaccharides
0
Hydrogels
0
Berberine
0I8Y3P32UF
Anti-Bacterial Agents
0
Anti-Inflammatory Agents
0
Antioxidants
0
Borates
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
22135Subventions
Organisme : Zhejiang Provincial Public Welfare Research Project
ID : LGF22E030007
Organisme : Zhejiang Medical Science and Technology Program
ID : 2020RC085
Organisme : Zhejiang Traditional Chinese Medicine Scientific Research Program
ID : 2021ZQ024
Informations de copyright
© 2024. The Author(s).
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