Anti-fibrogenic effect of umbilical cord-derived mesenchymal stem cell-conditioned media in human esophageal fibroblasts.

Humans Mesenchymal Stem Cells / metabolism Culture Media, Conditioned / pharmacology Transforming Growth Factor beta1 / metabolism rhoA GTP-Binding Protein / metabolism Esophagus / metabolism Fibroblasts / metabolism Trans-Activators / metabolism Transcription Factors / metabolism Fibrosis Umbilical Cord / cytology YAP-Signaling Proteins / metabolism Serum Response Factor / metabolism Adaptor Proteins, Signal Transducing / metabolism Signal Transduction Transcriptional Coactivator with PDZ-Binding Motif Proteins / metabolism Cells, Cultured Extracellular Matrix / metabolism Smad2 Protein / metabolism
Conditioned medium Esophageal fibrosis Mesenchymal stem cells Umbilical cord

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
27 Sep 2024
Historique:
received: 18 03 2024
accepted: 13 09 2024
medline: 28 9 2024
pubmed: 28 9 2024
entrez: 27 9 2024
Statut: epublish

Résumé

Esophageal fibrosis can develop due to caustic or radiation injuries. Umbilical cord-derived mesenchymal stem cells (UC-MSCs) are known to mitigate fibrosis in various organs. However, the potential effects of UC-MSCs on human esophageal fibrosis remain underexplored. This study investigated the anti-fibrogenic properties and mechanisms of UC-MSC-derived conditioned media (UC-MSC-CM) on human esophageal fibroblasts (HEFs). HEFs were treated with TGF-β1 and then cultured with UC-MSC-CM, and the expression levels of extracellular matrix (ECM) components, RhoA, myocardin related transcription factor A (MRTF-A), serum response factor (SRF), Yes-associated protein (YAP), and transcriptional coactivator with PDZ-binding motif (TAZ) were measured. UC-MSC-CM suppressed TGF-β1-induced fibrogenic activation in HEFs, as evidenced by the downregulation of ECM. UC-MSC-CM diminished the expression of RhoA, MRTF-A, and SRF triggered by TGF-β1. In TGF-β1-stimulated HEFs, UC-MSC-CM decreased the nuclear localization of MRTF-A and YAP. Additionally, UC-MSC-CM diminished the TGF-β1-induced nuclear expressions of YAP and TAZ, while concurrently enhancing the cytoplasmic presence of phosphorylated YAP. Furthermore, UC-MSC-CM reduced TGF-β1-induced phosphorylation of Smad2. These findings suggest that UC-MSC-CM may inhibit TGF-β1-induced fibrogenic activation in HEFs by targeting the Rho-mediated MRTF/SRF and YAP/TAZ pathways, as well as the Smad2 pathway. This indicates its potential as a stem cell therapy for esophageal fibrosis.

Identifiants

DOI: 10.1038/s41598-024-73091-7 PMID: 39333200
pubmed: 39333200
doi: 10.1038/s41598-024-73091-7
pii: 10.1038/s41598-024-73091-7
doi:

Substances chimiques

Culture Media, Conditioned 0
Transforming Growth Factor beta1 0
rhoA GTP-Binding Protein EC 3.6.5.2
Trans-Activators 0
Transcription Factors 0
MRTFA protein, human 0
YAP-Signaling Proteins 0
YAP1 protein, human 0
Serum Response Factor 0
Adaptor Proteins, Signal Transducing 0
Transcriptional Coactivator with PDZ-Binding Motif Proteins 0
WWTR1 protein, human 0
RHOA protein, human 124671-05-2
Smad2 Protein 0
SRF protein, human 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

22233

Informations de copyright

© 2024. The Author(s).

Références

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Auteurs

Yoon Jeong Choi (YJ)

Department of Gastroenterology, CHA Bundang Medical Center, CHA University School of Medicine, 59 Yatap-ro, Bundang-gu, Seongnam, 13496, South Korea.
Institute of Basic Medical Sciences, CHA University School of Medicine, Seongnam, 13496, South Korea.

Jee Hyun Kim (JH)

Department of Gastroenterology, CHA Bundang Medical Center, CHA University School of Medicine, 59 Yatap-ro, Bundang-gu, Seongnam, 13496, South Korea.

Yeonju Lee (Y)

Department of Gastroenterology, CHA Bundang Medical Center, CHA University School of Medicine, 59 Yatap-ro, Bundang-gu, Seongnam, 13496, South Korea.

Hee Jang Pyeon (HJ)

R&D Division, CHA Biotech Co., Ltd, Seongnam, 13488, South Korea.

In Kyung Yoo (IK)

Department of Gastroenterology, CHA Bundang Medical Center, CHA University School of Medicine, 59 Yatap-ro, Bundang-gu, Seongnam, 13496, South Korea. ikyoo82@hanmail.net.

Jun Hwan Yoo (JH)

Department of Gastroenterology, CHA Bundang Medical Center, CHA University School of Medicine, 59 Yatap-ro, Bundang-gu, Seongnam, 13496, South Korea. jhyoo@cha.ac.kr.
Institute of Basic Medical Sciences, CHA University School of Medicine, Seongnam, 13496, South Korea. jhyoo@cha.ac.kr.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Anti-fibrogenic effect of umbilical...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches multipotentes › Cellules souches mésenchymateuses › Anti-fibrogenic effect of umbilical...
questionsmedicales.fr › Équipement et fournitures › Milieux de culture › Milieux de culture conditionnés › Anti-fibrogenic effect of umbilical...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Protéines de la superfamille du TGF bêta › Facteur de croissance transformant bêta › Facteur de croissance transformant bêta-1 › Anti-fibrogenic effect of umbilical...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines G › Protéines G monomériques › Protéines G rho › Protéine G RhoA › Anti-fibrogenic effect of umbilical...
questionsmedicales.fr › Système digestif › Tube digestif › Tube digestif supérieur › Oesophage › Anti-fibrogenic effect of umbilical...
questionsmedicales.fr › Cellules › Cellules du tissu conjonctif › Fibroblastes › Anti-fibrogenic effect of umbilical...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Protéines virales régulatrices ou accessoires › Transactivateurs › Anti-fibrogenic effect of umbilical...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Anti-fibrogenic effect of umbilical...
questionsmedicales.fr › États, signes et symptômes pathologiques › Processus pathologiques › Fibrose › Anti-fibrogenic effect of umbilical...
questionsmedicales.fr › Structures de l'embryon › Foetus › Cordon ombilical › Anti-fibrogenic effect of umbilical...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines adaptatrices de la transduction du signal › Protéines de signalisation YAP › Anti-fibrogenic effect of umbilical...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Protéines à domaine MADS › Facteur de réponse au sérum › Anti-fibrogenic effect of umbilical...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines adaptatrices de la transduction du signal › Anti-fibrogenic effect of umbilical...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Transduction du signal › Anti-fibrogenic effect of umbilical...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines adaptatrices de la transduction du signal › Transcriptional coactivator with PDZ-binding motif proteins › Anti-fibrogenic effect of umbilical...
questionsmedicales.fr › Cellules › Cellules cultivées › Anti-fibrogenic effect of umbilical...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace extracellulaire › Matrice extracellulaire › Anti-fibrogenic effect of umbilical...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Protéines Smad › Protéines Smad régulées par les récepteurs › Protéine Smad2 › Anti-fibrogenic effect of umbilical...