Non-invasive label-free imaging analysis pipeline for in situ characterization of 3D brain organoids.
Brain organoids
Live imaging
Mesofluidics
Neurodevelopmental disorders
Non-invasive imaging
Quantitative phase imaging systems
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
27 Sep 2024
27 Sep 2024
Historique:
received:
08
03
2024
accepted:
03
09
2024
medline:
28
9
2024
pubmed:
28
9
2024
entrez:
27
9
2024
Statut:
epublish
Résumé
Brain organoids provide a unique opportunity to model organ development in a system similar to human organogenesis in vivo. Brain organoids thus hold great promise for drug screening and disease modeling. Conventional approaches to organoid characterization predominantly rely on molecular analysis methods, which are expensive, time-consuming, labor-intensive, and involve the destruction of the valuable three-dimensional (3D) architecture of the organoids. This reliance on end-point assays makes it challenging to assess cellular and subcellular events occurring during organoid development in their 3D context. As a result, the long developmental processes are not monitored nor assessed. The ability to perform non-invasive assays is critical for longitudinally assessing features of organoid development during culture. In this paper, we demonstrate a label-free high-content imaging approach for observing changes in organoid morphology and structural changes occurring at the cellular and subcellular level. Enabled by microfluidic-based culture of 3D cell systems and a novel 3D quantitative phase imaging method, we demonstrate the ability to perform non-destructive high-resolution quantitative image analysis of the organoid. The highlighted results demonstrated in this paper provide a new approach to performing live, non-destructive monitoring of organoid systems during culture.
Identifiants
pubmed: 39333572
doi: 10.1038/s41598-024-72038-2
pii: 10.1038/s41598-024-72038-2
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
22331Subventions
Organisme : National Science Foundation Graduate Research Fellowship Program
ID : NSF GRFP DGE-2039655
Organisme : NINDS NIH HHS
ID : R21NS117067
Pays : United States
Organisme : NIMH NIH HHS
ID : R21MH123711
Pays : United States
Organisme : U.S. Department of Defense
ID : W81XWH1910353
Organisme : Burroughs Wellcome Fund
ID : CASI BWF 1014540
Organisme : National Science Foundation
ID : NSF CBET CAREER 356 1752011
Organisme : NIGMS NIH HHS
ID : R35GM147437
Pays : United States
Informations de copyright
© 2024. The Author(s).
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