Trichomonas vaginalis: comparison of primers for implementation as an in-house PCR in rural Vellore, South India.
Trichomonas Vaginalis
AP65
BTUB
InPouch
Seegene Allplex
TVK 3/7
Journal
BMC infectious diseases
ISSN: 1471-2334
Titre abrégé: BMC Infect Dis
Pays: England
ID NLM: 100968551
Informations de publication
Date de publication:
27 Sep 2024
27 Sep 2024
Historique:
received:
25
04
2024
accepted:
15
07
2024
medline:
28
9
2024
pubmed:
28
9
2024
entrez:
28
9
2024
Statut:
epublish
Résumé
Trichomonas vaginalis (TV) accounts for the highest burden of curable, non-viral sexually transmitted infections worldwide. Prevalence in India ranges from 0.4 to 27.4% in women and 0.0-5.6% in men. In 2015, the prevalence of TV among pregnant women of rural Vellore was 3.11% using Sekisui OSOM Six-hundred adult, sexually active women attending the Obstetrics-Gynaecology rural out-patient clinic the Rural Unit for Health and Social Affairs (RUHSA) from July 2020 - February 2021 were enrolled. A vaginal lateral and posterior fornix specimen was inoculated, onsite, into Biomed InPouch Nine specimens (9/600, 1.5%) were positive for TV. There was a 100% correlation between Biomed InPouch TV Current hospital-based prevalence of TV in rural Vellore was 1.5%. Repetitive DNA target TVK 3/7 was more sensitive than AP65 and BTUB 9/2 primers.
Sections du résumé
BACKGROUND
BACKGROUND
Trichomonas vaginalis (TV) accounts for the highest burden of curable, non-viral sexually transmitted infections worldwide. Prevalence in India ranges from 0.4 to 27.4% in women and 0.0-5.6% in men. In 2015, the prevalence of TV among pregnant women of rural Vellore was 3.11% using Sekisui OSOM
MATERIALS & METHODS
METHODS
Six-hundred adult, sexually active women attending the Obstetrics-Gynaecology rural out-patient clinic the Rural Unit for Health and Social Affairs (RUHSA) from July 2020 - February 2021 were enrolled. A vaginal lateral and posterior fornix specimen was inoculated, onsite, into Biomed InPouch
RESULTS
RESULTS
Nine specimens (9/600, 1.5%) were positive for TV. There was a 100% correlation between Biomed InPouch TV
CONCLUSION
CONCLUSIONS
Current hospital-based prevalence of TV in rural Vellore was 1.5%. Repetitive DNA target TVK 3/7 was more sensitive than AP65 and BTUB 9/2 primers.
Identifiants
pubmed: 39333912
doi: 10.1186/s12879-024-09619-z
pii: 10.1186/s12879-024-09619-z
doi:
Types de publication
Journal Article
Comparative Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
1039Informations de copyright
© 2024. The Author(s).
Références
Taylor M, Alonso-González M, Gómez B, Korenromp E, Broutet N, Taylor M, et al. World health organization global health sector strategy on sexually transmitted infections: an evidence-to-action summary for Colombia. Rev Colomb Obstet Ginecol. 2017;68(3):193–201.
doi: 10.18597/rcog.3071
pubmed: 31543554
Rowley J, Vander Hoorn S, Korenromp E, Low N, Unemo M, Abu-Raddad LJ, et al. Chlamydia, Gonorrhoea, trichomoniasis and syphilis: global prevalence and incidence estimates, 2016. Bull World Health Organ. 2019;97(8):548–P562.
doi: 10.2471/BLT.18.228486
pubmed: 31384073
pmcid: 6653813
Wiringa AE, Ness RB, Darville T, Beigi RH, Haggerty CL. Trichomonas Vaginalis, endometritis and sequelae among women with clinically suspected pelvic inflammatory disease. Sex Transm Infect. 2020;96(6):436–8.
doi: 10.1136/sextrans-2019-054079
pubmed: 31719170
Sood S, Mohanty S, Kapil A, Tolosa J, Mittal S. InPouch TV culture for detection of Trichomonas vaginalis. Indian J Med Res. 2007;125(4):567–71.
pubmed: 17598943
Workowski KA, Bachmann LH, Chan PA, Johnston CM, Muzny CA, Park I et al. Sexually Transmitted Infections Treatment Guidelines, 2021. 2021;70(4):192.
Kengne P, Veas F, Vidal N, Rey JL, Cuny G. Trichomonas vaginalis: repeated DNA target for highly sensitive and specific polymerase chain reaction diagnosis. Cell Mol Biol Noisy–Gd Fr. 1994;40(6):819–31.
Nabweyambo S, Kakaire O, Sowinski S, Okeng A, Ojiambo H, Kimeze J, et al. Very low sensitivity of wet mount microscopy compared to PCR against culture in the diagnosis of vaginal trichomoniasis in Uganda: a cross sectional study. BMC Res Notes. 2017;10(1):259.
doi: 10.1186/s13104-017-2581-1
pubmed: 28683790
pmcid: 5501264
Crucitti T, Van Dyck E, Tehe A, Abdellati S, Vuylsteke B, Buve A, et al. Comparison of culture and different PCR assays for detection of Trichomonas Vaginalis in self collected vaginal swab specimens. Sex Transm Infect. 2003;79(5):393–8.
doi: 10.1136/sti.79.5.393
pubmed: 14573835
pmcid: 1744760
Herath S, Balendran T, Herath A, Iddawela D, Wickramasinghe S. Comparison of diagnostic methods and analysis of socio-demographic factors associated with Trichomonas vaginalis infection in Sri Lanka. PLoS ONE. 2021;16(10):e0258556.
doi: 10.1371/journal.pone.0258556
pubmed: 34644344
pmcid: 8513885
Madico G, Quinn TC, Rompalo A, McKee KT, Gaydos CA. Diagnosis of Trichomonas vaginalis infection by PCR using vaginal swab samples. J Clin Microbiol. 1998;36(11):3205–10.
doi: 10.1128/JCM.36.11.3205-3210.1998
pubmed: 9774566
pmcid: 105302
Pai NP, Daher J, Prashanth HR, Shetty A, Sahni RD, Kannangai R et al. Will an innovative connected AideSmart! app-based multiplex, point-of-care screening strategy for HIV and related coinfections affect timely quality antenatal screening of rural Indian women? Results from a cross-sectional study in India. Sex Transm Infect [Internet]. 2018 Oct 15 [cited 2021 Aug 25]; https://sti.bmj.com/content/early/2018/10/15/sextrans-2017-053491 .
Domeika M, Zhurauskaya L, Savicheva A, Frigo N, Sokolovskiy E, Hallén A, et al. Guidelines for the laboratory diagnosis of trichomoniasis in East European countries. J Eur Acad Dermatol Venereol JEADV. 2010;24(10):1125–34.
doi: 10.1111/j.1468-3083.2010.03601.x
pubmed: 20236204
Khatoon R. Evaluation of Different Staining Techniques in the Diagnosis of Trichomonas vaginalis Infection in Females of Reproductive Age Group. J Clin Diagn Res [Internet]. 2014 [cited 2019 Nov 9]; http://jcdr.net/article_fulltext.asp?issn=0973-709x&year=2014&volume=8&issue=12&page=DC05&issn=0973-709x&id=5261
Arora BB, Maheshwari M, Devgan N, Arora DR. Prevalence of Trichomoniasis, vaginal candidiasis, genital herpes, Chlamydiasis, and actinomycosis among urban and rural women of Haryana, India. J Sex Transm Dis. 2014;2014:1–5.
doi: 10.1155/2014/963812
Hobbs MM, Lapple DM, Lawing LF, Schwebke JR, Cohen MS, Swygard H, et al. Methods for detection of Trichomonas Vaginalis in the Male partners of Infected women: implications for control of Trichomoniasis. J Clin Microbiol. 2006;44(11):3994–9.
doi: 10.1128/JCM.00952-06
pubmed: 16971646
pmcid: 1698299
Roth AM, Williams JA, Ly R, Curd K, Brooks D, Arno J, et al. Changing sexually transmitted infection screening protocol will result in Improved Case finding for Trichomonas Vaginalis among High-Risk Female populations. Sex Transm Dis. 2011;38(5):398–400.
doi: 10.1097/OLQ.0b013e318203e3ce
pubmed: 21217417
Singh S, Saha R, Suneja A, Das S. A hospital-based study on the prevalence of trichomoniasis and evaluation of accuracy of various diagnostic techniques. Trop Parasitol. 2020;10(2):124–9.
doi: 10.4103/tp.tp_1_23
pubmed: 33747880
Vijaya Mn D, Umashankar K, Sudha null, Nagure AG, Kavitha G. Prevalence of the trichomonas vaginalis infection in a tertiary care hospital in rural bangalore, southern India. J Clin Diagn Res JCDR. 2013;7(7):1401–3.
pubmed: 23998075
Dadwal R, Sharma N, Kanaujia R, Malhotra S, Chaudhry H, Rathore S, et al. Prevalence of Trichomonas vaginalis by polymerase chain reaction-based molecular method among symptomatic women from Northern India. Indian J Sex Transm Dis AIDS. 2023;44(1):40–4.
doi: 10.4103/ijstd.ijstd_21_22
pubmed: 37457518
pmcid: 10343104
Herath S, Fernando D, Jayasinge S. Risk factors of Trichomonas Vaginalis in women attending central sexually transmitted diseases Clinic Sri Lanka. Retrovirology. 2012;9(1):P42.
doi: 10.1186/1742-4690-9-S1-P42
pmcid: 3360438
Radonjic IV, Dzamic AM, Mitrovic SM, Arsic Arsenijevic VS, Popadic DM, Kranjcic Zec IF. Diagnosis of Trichomonas vaginalis infection: the sensitivities and specificities of microscopy, culture and PCR assay. Eur J Obstet Gynecol Reprod Biol. 2006;126(1):116–20.
doi: 10.1016/j.ejogrb.2005.07.033
pubmed: 16249051
Khatoon R, Jahan N, Ahmad S, Khan HM, Rabbani T. Comparison of four diagnostic techniques for detection of Trichomonas vaginalis infection in females attending tertiary care hospital of North India. Indian J Pathol Microbiol. 2015;58(1):36–9.
doi: 10.4103/0377-4929.151172
pubmed: 25673589
Patil MJ, Nagamoti JM, Metgud SC. Diagnosis of Trichomonas Vaginalis from vaginal specimens by Wet Mount Microscopy, in Pouch TV Culture System, and PCR. J Glob Infect Dis. 2012;4(1):22–5.
doi: 10.4103/0974-777X.93756
pubmed: 22529623
pmcid: 3326953
Queza MIP, Rivera WL. Diagnosis and molecular characterization of Trichomonas Vaginalis in sex workers in the Philippines. Pathog Glob Health. 2013;107(3):136–40.
doi: 10.1179/2047773213Y.0000000085
pubmed: 23683368
pmcid: 4003591
Paul H, Peter D, Pulimood SA, Abraham OC, Mathai E, Prasad JH, et al. Role of polymerase chain reaction in the diagnosis of Trichomonas vaginalis infection in human immunodeficiency virus-infected individuals from India (South). Indian J Dermatol Venereol Leprol. 2012;78(3):323–7.
doi: 10.4103/0378-6323.95448
pubmed: 22565432
Lawing LF, Hedges SR, Schwebke JR. Detection of trichomonosis in vaginal and urine specimens from women by Culture and PCR. J Clin Microbiol. 2000;38(10):3585–8.
doi: 10.1128/JCM.38.10.3585-3588.2000
pubmed: 11015368
pmcid: 87441
Pillay A, Radebe F, Fehler G, Htun Y, Ballard RC. Comparison of a TaqMan-based real‐time polymerase chain reaction with conventional tests for the detection of Trichomonas vaginalis. Sex Transm Infect. 2007;83(2):126–9.
doi: 10.1136/sti.2006.022376
pubmed: 17090567
Wendel KA, Erbelding EJ, Gaydos CA, Rompalo AM. Trichomonas vaginalis polymerase chain reaction compared with Standard Diagnostic and Therapeutic Protocols for Detection and Treatment of Vaginal Trichomoniasis. Clin Infect Dis. 2002;35(5):576–80.
doi: 10.1086/342060
pubmed: 12173132
Mayta H, Gilman RH, Calderon MM, Gottlieb A, Soto G, Tuero I, et al. 18S ribosomal DNA-Based PCR for diagnosis of Trichomonas vaginalis. J Clin Microbiol. 2000;38(7):2683–7.
doi: 10.1128/JCM.38.7.2683-2687.2000
pubmed: 10878064
pmcid: 86998
Felleisen RS. Comparative sequence analysis of 5.8S rRNA genes and internal transcribed spacer (ITS) regions of trichomonadid protozoa. Parasitology. 1997;115(Pt 2):111–9.
doi: 10.1017/S0031182097001212
pubmed: 10190167
Masha SC, Wahome E, Vaneechoutte M, Cools P, Crucitti T, Sanders EJ. High prevalence of curable sexually transmitted infections among pregnant women in a rural county hospital in Kilifi, Kenya. PLoS ONE. 2017;12(3):e0175166.
doi: 10.1371/journal.pone.0175166
pubmed: 28362869
pmcid: 5375155
da Luz Becker D, dos Santos O, Frasson AP, de Vargas Rigo G, Macedo AJ, Tasca T. High rates of double-stranded RNA viruses and Mycoplasma hominis in Trichomonas Vaginalis clinical isolates in South Brazil. Infect Genet Evol J Mol Epidemiol Evol Genet Infect Dis. 2015;34:181–7.
Goo YK, Shin WS, Yang HW, Joo SY, Song SM, Ryu JS, et al. Prevalence of Trichomonas Vaginalis in Women visiting 2 obstetrics and gynecology clinics in Daegu, South Korea. Korean J Parasitol. 2016;54(1):75–80.
doi: 10.3347/kjp.2016.54.1.75
pubmed: 26951983
pmcid: 4792318
Ginocchio CC, Chapin K, Smith JS, Aslanzadeh J, Snook J, Hill CS, et al. Prevalence of Trichomonas Vaginalis and coinfection with Chlamydia trachomatis and Neisseria gonorrhoeae in the United States as determined by the Aptima trichomonas Vaginalis nucleic acid amplification assay. J Clin Microbiol. 2012;50(8):2601–8.
doi: 10.1128/JCM.00748-12
pubmed: 22622447
pmcid: 3421522