Factors influencing survival in sphingosine phosphate lyase insufficiency syndrome: a retrospective cross-sectional natural history study of 76 patients.


Journal

Orphanet journal of rare diseases
ISSN: 1750-1172
Titre abrégé: Orphanet J Rare Dis
Pays: England
ID NLM: 101266602

Informations de publication

Date de publication:
27 Sep 2024
Historique:
received: 28 11 2023
accepted: 06 08 2024
medline: 28 9 2024
pubmed: 28 9 2024
entrez: 28 9 2024
Statut: epublish

Résumé

Sphingosine-1-phosphate lyase insufficiency syndrome (SPLIS) is a recently recognized inborn error of metabolism associated with steroid-resistant nephrotic syndrome as well as adrenal insufficiency and immunological, neurological, and skin manifestations. SPLIS is caused by inactivating mutations in SGPL1, encoding the pyridoxal 5'phosphate-dependent enzyme sphingosine-1-phosphate lyase, which catalyzes the final step of sphingolipid metabolism. Some SPLIS patients have undergone kidney transplantation, and others have been treated with vitamin B6 supplementation. In addition, targeted therapies including gene therapy are in preclinical development. In anticipation of clinical trials, it will be essential to characterize the full spectrum and natural history of SPLIS. We performed a retrospective analysis of 76 patients in whom the diagnosis of SPLIS was established in a proband with at least one suggestive finding and biallelic SGPL1 variants identified by molecular genetic testing. The main objective of the study was to identify factors influencing survival in SPLIS subjects. Overall survival at last report was 50%. Major influences on survival included: (1) age and organ involvement at first presentation; (2) receiving a kidney transplant, and (3) SGPL1 genotype. Among 48 SPLIS patients with nephropathy who had not received a kidney transplant, two clinical subgroups were distinguished. Of children diagnosed with SPLIS nephropathy before age one (n = 30), less than 30% were alive 2 years after diagnosis, and 17% were living at last report. Among those diagnosed at or after age one (n = 18), ~ 70% were alive 2 years after diagnosis, and 72% were living at time of last report. SPLIS patients homozygous for the SPL R222Q variant survived longer compared to patients with other genotypes. Kidney transplantation significantly extended survival outcomes. Our results demonstrate that SPLIS is a phenotypically heterogeneous condition. We find that patients diagnosed with SPLIS nephropathy in the first year of life and patients presenting with prenatal findings represent two high-risk subgroups, whereas patients harboring the R222Q SGPL1 variant fare better than the rest. Time to progression from onset of proteinuria to end stage kidney disease varies from less than one month to five years, and kidney transplantation may be lifesaving.

Sections du résumé

BACKGROUND BACKGROUND
Sphingosine-1-phosphate lyase insufficiency syndrome (SPLIS) is a recently recognized inborn error of metabolism associated with steroid-resistant nephrotic syndrome as well as adrenal insufficiency and immunological, neurological, and skin manifestations. SPLIS is caused by inactivating mutations in SGPL1, encoding the pyridoxal 5'phosphate-dependent enzyme sphingosine-1-phosphate lyase, which catalyzes the final step of sphingolipid metabolism. Some SPLIS patients have undergone kidney transplantation, and others have been treated with vitamin B6 supplementation. In addition, targeted therapies including gene therapy are in preclinical development. In anticipation of clinical trials, it will be essential to characterize the full spectrum and natural history of SPLIS. We performed a retrospective analysis of 76 patients in whom the diagnosis of SPLIS was established in a proband with at least one suggestive finding and biallelic SGPL1 variants identified by molecular genetic testing. The main objective of the study was to identify factors influencing survival in SPLIS subjects.
RESULTS RESULTS
Overall survival at last report was 50%. Major influences on survival included: (1) age and organ involvement at first presentation; (2) receiving a kidney transplant, and (3) SGPL1 genotype. Among 48 SPLIS patients with nephropathy who had not received a kidney transplant, two clinical subgroups were distinguished. Of children diagnosed with SPLIS nephropathy before age one (n = 30), less than 30% were alive 2 years after diagnosis, and 17% were living at last report. Among those diagnosed at or after age one (n = 18), ~ 70% were alive 2 years after diagnosis, and 72% were living at time of last report. SPLIS patients homozygous for the SPL R222Q variant survived longer compared to patients with other genotypes. Kidney transplantation significantly extended survival outcomes.
CONCLUSION CONCLUSIONS
Our results demonstrate that SPLIS is a phenotypically heterogeneous condition. We find that patients diagnosed with SPLIS nephropathy in the first year of life and patients presenting with prenatal findings represent two high-risk subgroups, whereas patients harboring the R222Q SGPL1 variant fare better than the rest. Time to progression from onset of proteinuria to end stage kidney disease varies from less than one month to five years, and kidney transplantation may be lifesaving.

Identifiants

pubmed: 39334450
doi: 10.1186/s13023-024-03311-w
pii: 10.1186/s13023-024-03311-w
doi:

Substances chimiques

Aldehyde-Lyases EC 4.1.2.-
SGPL1 protein, human EC 4.1.2.27

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

355

Subventions

Organisme : National Institute of Child Health and Human Development
ID : 1R01HD113778

Informations de copyright

© 2024. The Author(s).

Références

Weaver KN, Sullivan B, Hildebrandt F, et al. Sphingosine phosphate lyase insufficiency syndrome. In: Adam MP, Ardinger HH, Pagon RA, et al., eds. GeneReviews. Seattle (WA), 2020.
Choi YJ, Saba JD. Sphingosine phosphate lyase insufficiency syndrome (SPLIS): a novel inborn error of sphingolipid metabolism. Adv Biol Regul. 2019;71:128–40. https://doi.org/10.1016/j.jbior.2018.09.004 .
doi: 10.1016/j.jbior.2018.09.004 pubmed: 30274713
Pournasiri Z, Madani A, Nazarpack F, et al. Sphingosine phosphate lyase insufficiency syndrome: a systematic review. World J Pediatr. 2022. https://doi.org/10.1007/s12519-022-00615-4 .
doi: 10.1007/s12519-022-00615-4 pubmed: 36371483
Tastemel Ozturk T, Canpolat N, Saygili S, et al. A rare cause of nephrotic syndrome-sphingosine-1-phosphate lyase (SGPL1) deficiency: 6 cases and a review of the literature. Pediatr Nephrol. 2023;38(3):711–9. https://doi.org/10.1007/s00467-022-05656-5 .
doi: 10.1007/s00467-022-05656-5 pubmed: 35748945
Yang S, He Y, Zhou J, Yuan H, Qiu L. Steroid-resistant nephrotic syndrome associated with certain SGPL1 variants in a family: Case report and literature review. Front Pediatr. 2023;11:1079758. https://doi.org/10.3389/fped.2023.1079758 .
doi: 10.3389/fped.2023.1079758 pubmed: 36873630 pmcid: 9978203
Maharaj A, Kwong R, Williams J, et al. A retrospective analysis of endocrine disease in sphingosine-1-phosphate lyase insufficiency: case series and literature review. Endocr Connect. 2022. https://doi.org/10.1530/EC-22-0250 .
doi: 10.1530/EC-22-0250 pubmed: 35904228 pmcid: 9346324
Saba JD. Fifty years of lyase and a moment of truth: sphingosine phosphate lyase from discovery to disease. J Lipid Res. 2019;60(3):456–63. https://doi.org/10.1194/jlr.S091181 .
doi: 10.1194/jlr.S091181 pubmed: 30635364 pmcid: 6399507
Van Veldhoven PP. Sphingosine-1-phosphate lyase. Methods Enzymol. 2000;311:244–54.
doi: 10.1016/S0076-6879(00)11087-0 pubmed: 10563331
Atkinson D, Nikodinovic Glumac J, Asselbergh B, et al. Sphingosine 1-phosphate lyase deficiency causes Charcot-Marie-Tooth neuropathy. Neurology. 2017;88(6):533–42. https://doi.org/10.1212/WNL.0000000000003595 .
doi: 10.1212/WNL.0000000000003595 pubmed: 28077491 pmcid: 5304460
Zhao P, Liu ID, Hodgin JB, et al. Responsiveness of sphingosine phosphate lyase insufficiency syndrome to vitamin B6 cofactor supplementation. J Inherit Metab Dis. 2020;43(5):1131–42. https://doi.org/10.1002/jimd.12238 .
doi: 10.1002/jimd.12238 pubmed: 32233035 pmcid: 8072405
Bektas M, Allende ML, Lee BG, et al. Sphingosine 1-phosphate lyase deficiency disrupts lipid homeostasis in liver. J Biol Chem. 2010;285(14):10880–9. https://doi.org/10.1074/jbc.M109.081489 .
doi: 10.1074/jbc.M109.081489 pubmed: 20097939 pmcid: 2856294
Zhao P, Tassew GB, Lee JY, et al. Efficacy of AAV9-mediated SGPL1 gene transfer in a mouse model of S1P lyase insufficiency syndrome. JCI Insight. 2021. https://doi.org/10.1172/jci.insight.145936 .
doi: 10.1172/jci.insight.145936 pubmed: 34877932 pmcid: 8783682
Kihara A. Sphingosine 1-phosphate is a key metabolite linking sphingolipids to glycerophospholipids. Biochim Biophys Acta. 2014;1841(5):766–72. https://doi.org/10.1016/j.bbalip.2013.08.014 .
doi: 10.1016/j.bbalip.2013.08.014 pubmed: 23994042
Uranbileg B, Kurano M, Kano K, et al. Sphingosine 1-phosphate lyase facilitates cancer progression through converting sphingolipids to glycerophospholipids. Clin Transl Med. 2022;12(9):e1056. https://doi.org/10.1002/ctm2.1056 .
doi: 10.1002/ctm2.1056 pubmed: 36125914 pmcid: 9488530
Mitroi DN, Karunakaran I, Graler M, et al. SGPL1 (sphingosine phosphate lyase 1) modulates neuronal autophagy via phosphatidylethanolamine production. Autophagy. 2017;13(5):885–99. https://doi.org/10.1080/15548627.2017.1291471 .
doi: 10.1080/15548627.2017.1291471 pubmed: 28521611 pmcid: 5446076
Mitroi DN, Deutschmann AU, Raucamp M, et al. Sphingosine 1-phosphate lyase ablation disrupts presynaptic architecture and function via an ubiquitin- proteasome mediated mechanism. Sci Rep. 2016;6:37064. https://doi.org/10.1038/srep37064 .
doi: 10.1038/srep37064 pubmed: 27883090 pmcid: 5121647
Alam S, Piazzesi A, Abd El Fatah M, Raucamp M, van Echten-Deckert G. Neurodegeneration caused by S1P-lyase deficiency involves calcium-dependent tau pathology and abnormal histone acetylation. Cells. 2020;9(10):2189. https://doi.org/10.3390/cells9102189 .
doi: 10.3390/cells9102189 pubmed: 32998447 pmcid: 7599816
Kim YI, Park K, Kim JY, et al. An endoplasmic reticulum stress-initiated sphingolipid metabolite, ceramide-1-phosphate, regulates epithelial innate immunity by stimulating beta-defensin production. Mol Cell Biol. 2014;34(24):4368–78. https://doi.org/10.1128/MCB.00599-14 .
doi: 10.1128/MCB.00599-14 pubmed: 25312644 pmcid: 4248733
Elias PM, Williams ML, Crumrine D, Schmuth M. Inherited clinical disorders of lipid metabolism. Curr Probl Dermatol. 2010;39:30–88. https://doi.org/10.1159/000321084 .
doi: 10.1159/000321084 pubmed: 20838037
Behne M, Uchida Y, Seki T, de Montellano PO, Elias PM, Holleran WM. Omega-hydroxyceramides are required for corneocyte lipid envelope (CLE) formation and normal epidermal permeability barrier function. J Investig Dermatol. 2000;114(1):185–92. https://doi.org/10.1046/j.1523-1747.2000.00846.x .
doi: 10.1046/j.1523-1747.2000.00846.x pubmed: 10620136
Smith CJ, Williams JL, Hall C, et al. Ichthyosis linked to sphingosine 1-phosphate lyase insufficiency is due to aberrant sphingolipid and calcium regulation. J Lipid Res. 2023;64(4):100351. https://doi.org/10.1016/j.jlr.2023.100351 .
doi: 10.1016/j.jlr.2023.100351 pubmed: 36868360 pmcid: 10123262
Bamborschke D, Pergande M, Becker K, et al. A novel mutation in sphingosine-1-phosphate lyase causing congenital brain malformation. Brain Dev. 2018;40(6):480–3. https://doi.org/10.1016/j.braindev.2018.02.008 .
doi: 10.1016/j.braindev.2018.02.008 pubmed: 29501407
Lovric S, Goncalves S, Gee HY, et al. Mutations in sphingosine-1-phosphate lyase cause nephrosis with ichthyosis and adrenal insufficiency. J Clin Invest. 2017;127(3):912–28. https://doi.org/10.1172/JCI89626 .
doi: 10.1172/JCI89626 pubmed: 28165339 pmcid: 5330730
Prasad R, Hadjidemetriou I, Maharaj A, et al. Sphingosine-1-phosphate lyase mutations cause primary adrenal insufficiency and steroid-resistant nephrotic syndrome. J Clin Invest. 2017;127(3):942–53. https://doi.org/10.1172/JCI90171 .
doi: 10.1172/JCI90171 pubmed: 28165343 pmcid: 5330744
Atreya KB, Saba JD. Neurological consequences of sphingosine phosphate lyase insufficiency. Front Cell Neurosci. 2022;16:938693. https://doi.org/10.3389/fncel.2022.938693 .
doi: 10.3389/fncel.2022.938693 pubmed: 36187293 pmcid: 9519528
Martin KW, Weaver N, Alhasan K, et al. MRI spectrum of brain involvement in sphingosine-1-phosphate lyase insufficiency syndrome. AJNR Am J Neuroradiol. 2020;41(10):1943–8. https://doi.org/10.3174/ajnr.A6746 .
doi: 10.3174/ajnr.A6746 pubmed: 32855188 pmcid: 7661081
Robinson PN, Kohler S, Bauer S, Seelow D, Horn D, Mundlos S. The Human Phenotype Ontology: a tool for annotating and analyzing human hereditary disease. Am J Hum Genet. 2008;83(5):610–5. https://doi.org/10.1016/j.ajhg.2008.09.017 .
doi: 10.1016/j.ajhg.2008.09.017 pubmed: 18950739 pmcid: 2668030
Khan R, Oskouian B, Lee JY, et al. AAV-SPL 2.0, a modified adeno-associated virus gene therapy agent for the treatment of sphingosine phosphate lyase insufficiency syndrome. Int J Mol Sci. 2023;24(21):15560. https://doi.org/10.3390/ijms242115560 .
doi: 10.3390/ijms242115560 pubmed: 37958544 pmcid: 10648410
Pizzamiglio C, Vernon H, Hanna M, Pitceathly R. Designing clinical trials for rare diseases: unique challenges and opportunities. Nat Rev Methods Primers 2022;2(1)
Administration FaD. Rare diseases: Natural history studies for drug development. Draft guidance for industry: Office of the Commissioner, Office of Clinical Policy and Programs, Office of Orphan Products Development, Center for Druge Evaluation and Research, Center for Biologics Evaluation Research, 2019.
Ranch D. Proteinuria in children. Pediatr Ann. 2020;49(6):e268–72. https://doi.org/10.3928/19382359-20200520-04 .
doi: 10.3928/19382359-20200520-04 pubmed: 32520368
Richards S, Aziz N, Bale S, et al. Standards and guidelines for the interpretation of sequence variants: a joint consensus recommendation of the American College of Medical Genetics and Genomics and the Association for Molecular Pathology. Genet Med. 2015;17(5):405–24. https://doi.org/10.1038/gim.2015.30 .
doi: 10.1038/gim.2015.30 pubmed: 25741868 pmcid: 4544753
Kopanos C, Tsiolkas V, Kouris A, et al. VarSome: the human genomic variant search engine. Bioinformatics. 2019;35(11):1978–80. https://doi.org/10.1093/bioinformatics/bty897 .
doi: 10.1093/bioinformatics/bty897 pubmed: 30376034
Sedillo J, Badduke C, Schrodi S, et al. Prevalence estimate of sphingosine phosphate lyase insufficiency syndrome in worldwide and select populations. Genet Med Open. 2024;2:100840.
doi: 10.1016/j.gimo.2023.100840
Spizzirri AP, Cobenas CJ, Suarez ADC. A rare cause of nephrotic syndrome - sphingosine-1-phosphate lyase (SGPL1) deficiency: 2 cases. Pediatr Nephrol. 2023;38(1):307–8. https://doi.org/10.1007/s00467-022-05716-w .
doi: 10.1007/s00467-022-05716-w pubmed: 35997975
Saygili S, Canpolat N, Sever L, Caliskan S, Atayar E, Ozaltin F. Persistent hypoglycemic attacks during hemodialysis sessions in an infant with congenital nephrotic syndrome: answers. Pediatr Nephrol. 2019;34(1):77–9. https://doi.org/10.1007/s00467-018-3982-7 .
doi: 10.1007/s00467-018-3982-7 pubmed: 29959533
Seven Menevse T, Kendir Demirkol Y, Gurpinar Tosun B, et al. Steroid hormone profiles and molecular diagnostic tools in pediatric patients with non-CAH primary adrenal insufficiency. J Clin Endocrinol Metab. 2022;107(5):e1924–31. https://doi.org/10.1210/clinem/dgac016 .
doi: 10.1210/clinem/dgac016 pubmed: 35028661
Pezzuti IL, Silva IN, Albuquerque CT, Duarte MG, Silva JM. Adrenal insufficiency in association with congenital nephrotic syndrome: a case report. J Pediatr Endocrinol Metab. 2014;27(5–6):565–7. https://doi.org/10.1515/jpem-2011-0296 .
doi: 10.1515/jpem-2011-0296 pubmed: 24633749
Linhares N, Arantes R, Araujo S, Pena S. Nephrotic syndrome and adrenal insufficiency caused by a variant in SGPL1. Clin Kidney J. 2017;11:1–6.
Janecke AR, Xu R, Steichen-Gersdorf E, et al. Deficiency of the sphingosine-1-phosphate lyase SGPL1 is associated with congenital nephrotic syndrome and congenital adrenal calcifications. Hum Mutat. 2017;38(4):365–72. https://doi.org/10.1002/humu.23192 .
doi: 10.1002/humu.23192 pubmed: 28181337 pmcid: 5384969
Settas N, Persky R, Faucz FR, et al. SGPL1 deficiency: a rare cause of primary adrenal insufficiency. J Clin Endocrinol Metab. 2018. https://doi.org/10.1210/jc.2018-02238 .
doi: 10.1210/jc.2018-02238 pmcid: 6435096
Roa-Bautista A, Sohail M, Wakeling E, et al. Combined novel homozygous variants in both SGPL1 and STAT1 presenting with severe combined immune deficiency: case report and literature review. Front Immunol. 2023;14:1186575. https://doi.org/10.3389/fimmu.2023.1186575 .
doi: 10.3389/fimmu.2023.1186575 pubmed: 37377976 pmcid: 10291229
Najafi M, Riedhammer KM, Rad A, et al. High detection rate for disease-causing variants in a cohort of 30 Iranian pediatric steroid resistant nephrotic syndrome cases. Front Pediatr. 2022;10:974840. https://doi.org/10.3389/fped.2022.974840 .
doi: 10.3389/fped.2022.974840 pubmed: 36245711 pmcid: 9555279
Ron HA, Scobell R, Strong A, Salazar EG, Ganetzky R. Congenital adrenal calcifications as the first clinical indication of sphingosine lyase insufficiency syndrome: a case report and review of the literature. Am J Med Genet A. 2022;188(11):3312–7. https://doi.org/10.1002/ajmg.a.62956 .
doi: 10.1002/ajmg.a.62956 pubmed: 35972040 pmcid: 9548492
Mathew G, Yasmeen MS, Deepthi RV, et al. Infantile nephrotic syndrome, immunodeficiency and adrenal insufficiency-a rare cause: answers. Pediatr Nephrol. 2022;37(4):817–9. https://doi.org/10.1007/s00467-021-05377-1 .
doi: 10.1007/s00467-021-05377-1 pubmed: 34999987
Mathew G, Yasmeen MS, Deepthi RV, et al. Infantile nephrotic syndrome, immunodeficiency and adrenal insufficiency-a rare cause: questions. Pediatr Nephrol. 2022;37(4):813–5. https://doi.org/10.1007/s00467-021-05357-5 .
doi: 10.1007/s00467-021-05357-5 pubmed: 34999981
Buyukyilmaz G, Adiguzel KT, Aksoy OY, et al. Novel sphingosine-1-phosphate lyase mutation causes multisystemic diseases: case report. Turk J Pediatr. 2023;65(6):1025–32. https://doi.org/10.24953/turkjped.2022.187 .
doi: 10.24953/turkjped.2022.187 pubmed: 38204317
Tran P, Jamee M, Pournasiri Z, Chavoshzadeh Z, Sullivan KE. SGPL1 deficiency: nephrotic syndrome with lymphopenia. J Clin Immunol. 2022. https://doi.org/10.1007/s10875-022-01348-9 .
doi: 10.1007/s10875-022-01348-9 pubmed: 36050428 pmcid: 8976534
Taylor VA, Stone HK, Schuh MP, Zhao X, Setchell KD, Erkan E. Disarranged sphingolipid metabolism from sphingosine-1-phosphate lyase deficiency leads to congenital nephrotic syndrome. Kidney Int Rep. 2019;4(12):1763–9. https://doi.org/10.1016/j.ekir.2019.07.018 .
doi: 10.1016/j.ekir.2019.07.018 pubmed: 31844815 pmcid: 6895586
Reiss U, Oskouian B, Zhou J, et al. Sphingosine-phosphate lyase enhances stress-induced ceramide generation and apoptosis. J Biol Chem. 2004;279(2):1281–90.
doi: 10.1074/jbc.M309646200 pubmed: 14570870
Bierzynska A, McCarthy HJ, Soderquest K, et al. Genomic and clinical profiling of a national nephrotic syndrome cohort advocates a precision medicine approach to disease management. Kidney Int. 2017;91(4):937–47. https://doi.org/10.1016/j.kint.2016.10.013 .
doi: 10.1016/j.kint.2016.10.013 pubmed: 28117080
Saba JD, Keller N, Wang JY, Tang F, Slavin A, Shen Y. Genotype/phenotype interactions and first steps toward targeted therapy for sphingosine phosphate lyase insufficiency syndrome. Cell Biochem Biophys. 2021;79(3):547–59. https://doi.org/10.1007/s12013-021-01013-9 .
doi: 10.1007/s12013-021-01013-9 pubmed: 34133011

Auteurs

Nancy Keller (N)

Department of Pediatrics, University of California, San Francisco, CA, USA.

Julian Midgley (J)

Department of Nephrology, Alberta Children's Hospital, Calgary, AB, Canada.

Ehtesham Khalid (E)

Ochsner Clinical School, University of Queensland (Australia) and Ochsner Health, New Orleans, LA, USA.

Harry Lesmana (H)

Center for Personalized Genetic Healthcare and Department of Pediatric Hematology/Oncology and BMT, Cleveland Clinic, Cleveland, OH, USA.

Georgie Mathew (G)

Division of Pediatric Nephrology, Christian Medical College, Vellore, India.

Christine Mincham (C)

Department of Nephrology, Perth Children's Hospital, Perth, Australia.

Norbert Teig (N)

Department of Neonatology and Pediatric Intensive Care, Ruhr-Universität Bochum, Bochum, Germany.

Zubair Khan (Z)

Department of Pediatrics, NAMO Medical Education and Research Institute, Shri Vinoba Bhave Civil Hospital, Silvassa, Dadra and Nagar Haveli, Daman and Diu, India.

Indu Khosla (I)

Department of Pediatric Pulmonology and Sleep Medicine, NH SRCC Hospital for Children, Mumbai, India.

Sam Mehr (S)

Department of Immunology, Royal Children's Hospital, Melbourne, Australia.

Tulay Guran (T)

Department of Pediatric Endocrinology and Diabetes, Marmara University School of Medicine, Istanbul, Turkey.

Kathrin Buder (K)

Pediatric Nephrology Department, University Children's Hospital Zurich, Steinwiesstrasse 75, 8032, Zurich, Switzerland.
Department of General Pediatrics and Hematology/Oncology, University Hospital Tuebingen, University Children's Hospital, Hoppe-Seyler-Strasse 1, 72076, Tuebingen, Germany.

Hong Xu (H)

Department of Nephrology, Children's Hospital of Fudan University, National Pediatric Medical Center of China, Shanghai, China.

Khalid Alhasan (K)

Department of Pediatrics, College of Medicine, King Saud University, Riyadh, Saudi Arabia.

Gonul Buyukyilmaz (G)

Department of Pediatric Endocrinology, Ankara City Hospital, Ankara, Turkey.

Nicole Weaver (N)

Department of Pediatrics, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.

Julie D Saba (JD)

Department of Pediatrics, University of California, San Francisco, CA, USA. Julie.Saba@ucsf.edu.

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