Protocol for the processing, cryopreservation, and biobanking of patient-derived cells and tissues.

Cancer Cell isolation Clinical Protocol Health Sciences

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
27 Sep 2024
Historique:
received: 27 02 2024
revised: 22 06 2024
accepted: 13 08 2024
medline: 28 9 2024
pubmed: 28 9 2024
entrez: 28 9 2024
Statut: aheadofprint

Résumé

Biobanking of patient-derived specimens offers unique opportunities for retrospective testing that could potentially contribute to diagnosing and evaluating clinical conditions, advancing personalized medicine and translational biomedical discovery. In this protocol, we detail the collection, processing, and cryopreservation of peripheral blood, bone marrow, and lymph nodes from patients with hematological malignancies. This protocol can be used for multiomics to gain cellular and molecular insights into blood cancers and to test the therapeutic potential of compounds for translational biomedical research. For complete details on the use and execution of this protocol, please refer to Lim et al.

Identifiants

DOI: 10.1016/j.xpro.2024.103287 PMID: 39340776
pubmed: 39340776
pii: S2666-1667(24)00452-0
doi: 10.1016/j.xpro.2024.103287
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

103287

Informations de copyright

Crown Copyright © 2024. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests D.T. has received research funding from Janssen and Roche and honoraria from CSL, MSL, Takeda, Roche, Janssen, BeiGene, Antengene, and EUSA.

Auteurs

Abhimanu Pandey (A)

Division of Immunology and Infectious Disease, The John Curtin School of Medical Research, The Australian National University, Canberra, ACT 2601, Australia; Haematology Translational Research Unit, ACT Pathology, Canberra Health Services, Canberra, ACT 2605, Australia.

Shweta Venkataraman (S)

Division of Immunology and Infectious Disease, The John Curtin School of Medical Research, The Australian National University, Canberra, ACT 2601, Australia; Haematology Translational Research Unit, ACT Pathology, Canberra Health Services, Canberra, ACT 2605, Australia.

Si Ming Man (SM)

Division of Immunology and Infectious Disease, The John Curtin School of Medical Research, The Australian National University, Canberra, ACT 2601, Australia.

Dipti Talaulikar (D)

Division of Immunology and Infectious Disease, The John Curtin School of Medical Research, The Australian National University, Canberra, ACT 2601, Australia; Haematology Translational Research Unit, ACT Pathology, Canberra Health Services, Canberra, ACT 2605, Australia; Department of Diagnostic Genomics, ACT Pathology, Canberra, ACT 2605, Australia; School of Medicine and Psychology, College of Health and Medicine, The Australian National University, Canberra, ACT 2601, Australia. Electronic address: dipti.talaulikar@act.gov.au.

Classifications MeSH