Cytolysin A is an intracellularly induced and secreted cytotoxin of typhoidal Salmonella.
Humans
Bacterial Proteins
/ metabolism
Animals
Mice
Vacuoles
/ metabolism
Salmonella paratyphi A
/ metabolism
Macrophages
/ microbiology
Perforin
/ metabolism
Salmonella typhi
/ metabolism
Virulence Factors
/ metabolism
Erythrocytes
/ metabolism
Cytotoxins
/ metabolism
Epithelial Cells
/ metabolism
Bacterial Secretion Systems
/ metabolism
Gene Expression Regulation, Bacterial
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
28 Sep 2024
28 Sep 2024
Historique:
received:
16
01
2024
accepted:
20
09
2024
medline:
29
9
2024
pubmed:
29
9
2024
entrez:
28
9
2024
Statut:
epublish
Résumé
Typhoidal Salmonella enterica serovars, such as Typhi and Paratyphi A, cause severe systemic infections, thereby posing a significant threat as human-adapted pathogens. This study focuses on cytolysin A (ClyA), a virulence factor essential for bacterial dissemination within the human body. We show that ClyA is exclusively expressed by intracellular S. Paratyphi A within the Salmonella-containing vacuole (SCV), regulated by the PhoP/Q system and SlyA. ClyA localizes in the bacterial periplasm, suggesting potential secretion. Deletion of TtsA, an essential Type 10 Secretion System component, completely abolishes intracellular ClyA detection and its presence in host cell supernatants. Host cells infected with wild-type S. Paratyphi A contain substantial ClyA, with supernatants capable of lysing neighboring cells. Notably, ClyA selectively lyses macrophages and erythrocytes while sparing epithelial cells. These findings identify ClyA as an intracellularly induced cytolysin, dependent on the SCV environment and secreted via a Type 10 Secretion System, with specific cytolytic activity.
Identifiants
pubmed: 39341826
doi: 10.1038/s41467-024-52745-0
pii: 10.1038/s41467-024-52745-0
doi:
Substances chimiques
Bacterial Proteins
0
Perforin
126465-35-8
Virulence Factors
0
Cytotoxins
0
Bacterial Secretion Systems
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
8414Subventions
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : 523077943
Informations de copyright
© 2024. The Author(s).
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