Sample collecting methods for bacterial community structure analysis of scalp hair: non-invasive swabbing versus intrusive hair shaft cutting.

Humans Hair / microbiology Scalp / microbiology Microbiota Female Bacteria / isolation & purification Male Adult Specimen Handling / methods Middle Aged RNA, Ribosomal, 16S / genetics
Bacterial community structure Scalp Scalp hair Swabbing

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
28 Sep 2024
Historique:
received: 25 06 2024
accepted: 11 09 2024
medline: 29 9 2024
pubmed: 29 9 2024
entrez: 28 9 2024
Statut: epublish

Résumé

Human skin samples for microbiome analysis are traditionally collected using a non-invasive swabbing method. Here, we compared the differences in bacterial community structures on scalp hair and scalps with samples collected using non-invasive swabbing and cutting/removal of scalp hair in 12 individuals. Hair-related samples, such as hair shafts and hair swabs, had significantly higher alpha diversity than scalp swab samples, whereas there were no significant differences between hair shafts and hair swabs. The relative abundances of the three major phyla and five major operational taxonomic units were not significantly different between the hair shaft and hair swab samples. The principal coordinate analysis plots based on weighted UniFrac distances were grouped into two clusters: samples from hair-related areas and scalp swabs, and there were significant differences only between samples from hair-related areas and scalp swabs. In addition, a weighted UniFrac analysis revealed that the sampling site-based category was a statistical category but not a hair sampling method-based category. These results suggest that scalp hair bacteria collected using non-invasive swab sampling were comparable to those collected cutting/removal of scalp hair.

Identifiants

DOI: 10.1038/s41598-024-72936-5 PMID: 39341874
pubmed: 39341874
doi: 10.1038/s41598-024-72936-5
pii: 10.1038/s41598-024-72936-5
doi:

Substances chimiques

RNA, Ribosomal, 16S 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

22461

Subventions

Organisme : Japan Society for the Promotion of Science under a Grant-in-Aid for Research Activity Start-up
ID : JP22K20573

Informations de copyright

© 2024. The Author(s).

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Auteurs

Kota Watanabe (K)

Laboratory of Fermentation Microbiology, Department of Fermentation Science and Technology, Faculty of Applied Biosciences, Tokyo University of Agriculture, Sakuragaoka 1-1-1, Setagaya-ku, Tokyo, 156-8502, Japan. kw207917@nodai.ac.jp.

Azusa Yamada (A)

Laboratory of Soil and Environmental Microbiology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Motooka 744, Nishi-ku, Fukuoka, 819-0395, Japan.

Hao Masuda (H)

Laboratory of Fermentation Microbiology, Department of Fermentation Science and Technology, Faculty of Applied Biosciences, Tokyo University of Agriculture, Sakuragaoka 1-1-1, Setagaya-ku, Tokyo, 156-8502, Japan.

Eri Kashiwazaki (E)

Laboratory of Fermentation Microbiology, Department of Fermentation Science and Technology, Faculty of Applied Biosciences, Tokyo University of Agriculture, Sakuragaoka 1-1-1, Setagaya-ku, Tokyo, 156-8502, Japan.

Shunichi Nakayama (S)

Laboratory of Fermentation Microbiology, Department of Fermentation Science and Technology, Faculty of Applied Biosciences, Tokyo University of Agriculture, Sakuragaoka 1-1-1, Setagaya-ku, Tokyo, 156-8502, Japan.

Toshimori Kadokura (T)

Laboratory of Fermentation Microbiology, Department of Fermentation Science and Technology, Faculty of Applied Biosciences, Tokyo University of Agriculture, Sakuragaoka 1-1-1, Setagaya-ku, Tokyo, 156-8502, Japan.

Kenji Sakai (K)

Laboratory of Soil and Environmental Microbiology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Motooka 744, Nishi-ku, Fukuoka, 819-0395, Japan.
Laboratory of Microbial Environmental Protection, Tropical Microbiology Unit, Center for International Education and Research of Agriculture, Faculty of Agriculture, Kyushu University, Motooka 744, Nishi-ku, Fukuoka, 819-0395, Japan.

Yukihiro Tashiro (Y)

Laboratory of Soil and Environmental Microbiology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Motooka 744, Nishi-ku, Fukuoka, 819-0395, Japan.
Laboratory of Microbial Environmental Protection, Tropical Microbiology Unit, Center for International Education and Research of Agriculture, Faculty of Agriculture, Kyushu University, Motooka 744, Nishi-ku, Fukuoka, 819-0395, Japan.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Sample collecting methods for bacterial...
questionsmedicales.fr › Système tégumentaire › Poils › Sample collecting methods for bacterial...
questionsmedicales.fr › Régions du corps › Tête › Cuir chevelu › Sample collecting methods for bacterial...
questionsmedicales.fr › Environnement et santé publique › Environnement › Écosystème › Biodiversité › Biote › Microbiote › Sample collecting methods for bacterial...
questionsmedicales.fr › Bactéries › Sample collecting methods for bacterial...
questionsmedicales.fr › Personnes › Tranches d'âge › Adulte › Sample collecting methods for bacterial...
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Manipulation d'échantillons › Sample collecting methods for bacterial...
questionsmedicales.fr › Personnes › Tranches d'âge › Adulte › Adulte d'âge moyen › Sample collecting methods for bacterial...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN ribosomique › ARN ribosomique 16S › Sample collecting methods for bacterial...