Carboxy-terminal polyglutamylation regulates signaling and phase separation of the Dishevelled protein.
Dishevelled 3
Noncanonical Wnt Signaling
Polyglutamylation
Protein Condensates
TTLL11
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
30 Sep 2024
30 Sep 2024
Historique:
received:
13
11
2023
accepted:
16
09
2024
revised:
15
08
2024
medline:
1
10
2024
pubmed:
1
10
2024
entrez:
30
9
2024
Statut:
aheadofprint
Résumé
Polyglutamylation is a reversible posttranslational modification that is catalyzed by enzymes of the tubulin tyrosine ligase-like (TTLL) family. Here, we found that TTLL11 generates a previously unknown type of polyglutamylation that is initiated by the addition of a glutamate residue to the free C-terminal carboxyl group of a substrate protein. TTLL11 efficiently polyglutamylates the Wnt signaling protein Dishevelled 3 (DVL3), thereby changing the interactome of DVL3. Polyglutamylation increases the capacity of DVL3 to get phosphorylated, to undergo phase separation, and to act in the noncanonical Wnt pathway. Both carboxy-terminal polyglutamylation and the resulting reduction in phase separation capacity of DVL3 can be reverted by the deglutamylating enzyme CCP6, demonstrating a causal relationship between TTLL11-mediated polyglutamylation and phase separation. Thus, C-terminal polyglutamylation represents a new type of posttranslational modification, broadening the range of proteins that can be modified by polyglutamylation and providing the first evidence that polyglutamylation can modulate protein phase separation.
Identifiants
pubmed: 39349846
doi: 10.1038/s44318-024-00254-7
pii: 10.1038/s44318-024-00254-7
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Czech Science Foundation
ID : GX19-28347X
Organisme : Czech Science Foundation
ID : GA22-25365S
Organisme : Czech Science Foundation
ID : GA23-07149S
Organisme : Czech Science Foundation
ID : GA22-06405S
Organisme : Agence Nationale de la Recherche (ANR)
ID : ANR-10-IDEX-0001-02
Organisme : Agence Nationale de la Recherche (ANR)
ID : ANR-12-BSV2-0007
Organisme : Agence Nationale de la Recherche (ANR)
ID : ANR-17-CE13-0021
Organisme : LabEx Cell&Scale grant
ID : ANR-11-LBX-0038
Organisme : Institut de convergence Q-life grant
ID : ANR-17-CONV-0005
Organisme : Czech Academy of Sciences grant RVO
ID : 86652036
Organisme : Charles University Grant Agency
ID : 1414120
Organisme : MEYS CR
ID : LM2018129
Organisme : MEYS CR
ID : LM2018127
Organisme : European Regional Development Fund-Project
ID : CZ.02.1.01/0.0/0.0/18_046/0015974
Organisme : e-Infrastruktura CZ
ID : INFRA LM2018140
Organisme : Funded by the European Union - Next Generation EU - National Institute for Cancer Research
ID : LX22NPO5102
Informations de copyright
© 2024. The Author(s).
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