Lipase activated endocytosis-like behavior of oil-in-water emulsion.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
02 Oct 2024
02 Oct 2024
Historique:
received:
14
12
2023
accepted:
23
09
2024
medline:
2
10
2024
pubmed:
2
10
2024
entrez:
1
10
2024
Statut:
epublish
Résumé
Oil-in-water emulsion is a system with extensive applications in foods, cosmetics and coating industries, and it could also be designed into an artificial lipid droplet in recent works. However, the insights into the biophysical dynamic behaviors of such artificial lipid droplets are lacking. Here, we reveal an enzymatic reaction triggered endocytosis-like behavior in the oil-in-water emulsion lipid droplets. A thermodynamically favored recruitment of lipases onto the membrane of the droplets is demonstrated. We confirm that the hydrolysis of tributyrin by lipases can decrease the interfacial tension and increase the compressive force on the membrane, which are the two main driving forces for triggering the endocytosis-like behavior. The endocytosis-like behavior induced various emerging functionalities of the lipid droplets, including proteins, DNA or inorganic particles being efficiently sequestered into the oil droplet with reversible release as well as enhanced cascade enzymatic reaction. Overall, our studies are expected to open up a way to functionalize oil-in-water emulsions capable of life-inspired behaviors and tackle emerging challenges in bottom-up synthetic biology, revealing the unknown dynamic behaviors of lipid droplets in living organisms.
Identifiants
pubmed: 39353937
doi: 10.1038/s41467-024-52802-8
pii: 10.1038/s41467-024-52802-8
doi:
Substances chimiques
Emulsions
0
Lipase
EC 3.1.1.3
Water
059QF0KO0R
Triglycerides
0
tributyrin
S05LZ624MF
Oils
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
8517Subventions
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 22171058
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 22307030
Organisme : China Postdoctoral Science Foundation
ID : 2021M700998
Organisme : Heilongjiang Postdoctoral Science Foundation
ID : No. LBH-Z21055
Informations de copyright
© 2024. The Author(s).
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