Evaluation of enzymatic protocols to optimize efficiency of bovine adipose tissue-derived mesenchymal stromal cell isolation.
Journal
NPJ science of food
ISSN: 2396-8370
Titre abrégé: NPJ Sci Food
Pays: England
ID NLM: 101739627
Informations de publication
Date de publication:
01 Oct 2024
01 Oct 2024
Historique:
received:
23
11
2023
accepted:
24
09
2024
medline:
2
10
2024
pubmed:
2
10
2024
entrez:
1
10
2024
Statut:
epublish
Résumé
Sustainable food provision for a continuously growing human population is one of the major challenges for the next decades. Cultured meat represents one of the alternatives which is currently extensively explored. Yet, the most appropriate cell type, capable of long-term proliferation and myogenic differentiation, remains to be identified. Bovine mesenchymal stromal cells (MSCs) are considered as a promising cell source. Within the context of cultured meat production, it is mandatory to maximize cell yield per tissue source. Although many enzymatic methods to isolate MSCs from adipose tissue (AT) have been described, cell yield has never been compared. In this study, we evaluate 32 isolation conditions including four enzyme mixtures (Collagenase type I, Collagenase type I + Trypsin, Liberase
Identifiants
pubmed: 39353952
doi: 10.1038/s41538-024-00313-7
pii: 10.1038/s41538-024-00313-7
doi:
Types de publication
Journal Article
Langues
eng
Pagination
70Informations de copyright
© 2024. The Author(s).
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