Revisiting the catalytic activity of single horseradish peroxidase clusters through electrochemical collision technique: Effect of electrolyte and substrate.
Electrochemical collision technique
Horseradish peroxidase
Maximum turnover number
Mediated electron transfer
Michaelis-Menten kinetics
Journal
Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R
Informations de publication
Date de publication:
01 Oct 2024
01 Oct 2024
Historique:
received:
08
02
2024
revised:
04
09
2024
accepted:
24
09
2024
medline:
3
10
2024
pubmed:
3
10
2024
entrez:
2
10
2024
Statut:
aheadofprint
Résumé
Horseradish peroxidase (HRP) is a versatile biosensing label and signal reporter owing to its broad-spectrum catalytic ability. In present work, we characterized HRP's catalytic performance with various substrates using electrochemical collision technique and analyzed the associated electron transfer processes. Different electrolyte solutions greatly affected enzyme dispersibility and zeta potential, thereby impacting HRP collision dynamics in single H
Identifiants
pubmed: 39357400
pii: S0039-9140(24)01330-4
doi: 10.1016/j.talanta.2024.126951
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
126951Informations de copyright
Copyright © 2024 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.