The scaffolding protein IQGAP1 enhances EGFR signaling by Promoting Oligomerization and Preventing Degradation.

Journal

The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R

Informations de publication

Date de publication:
30 Sep 2024
Historique:
received: 17 06 2024
revised: 05 09 2024
accepted: 19 09 2024
medline: 3 10 2024
pubmed: 3 10 2024
entrez: 2 10 2024
Statut: aheadofprint

Résumé

IQGAP1 is a large, multi-domain scaffold that connects and modulates different signaling networks including the one initiated by epidermal growth factor (EGF). In this study, we have used live cell fluorescence imaging methods along with other biochemical techniques to follow the mechanisms used by IQGAP1 to enhance EGF signaling. We show that IQGAP1 enhances EGF signaling by promoting the oligomerization of its receptor, EGFR, upon EGF addition along with concurrent IQGAP oligomerization. Using cellular markers, we find that IQGAP1 promotes the plasma membrane localization of EGFR and promotes association to one of its phosphoinositide lipid pathway ligands, PI(3,4,5)P

Identifiants

DOI: 10.1016/j.jbc.2024.107844 PMID: 39357822
pubmed: 39357822
pii: S0021-9258(24)02346-9
doi: 10.1016/j.jbc.2024.107844
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

107844

Informations de copyright

Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Conflicts of Interest: The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

Auteurs

V Siddartha Yerramilli (VS)

Department of Chemistry and Biochemistry, Worcester Polytechnic Institute, 100 Institute Rd., Worcester, MA 01605, USA.

Guanyu Lin (G)

Department of Chemistry and Biochemistry, Worcester Polytechnic Institute, 100 Institute Rd., Worcester, MA 01605, USA.

Jessica L Reisinger (JL)

Department of Chemistry and Biochemistry, Worcester Polytechnic Institute, 100 Institute Rd., Worcester, MA 01605, USA.

Rachel M Hemmerlin (RM)

Department of Chemistry and Biochemistry, Worcester Polytechnic Institute, 100 Institute Rd., Worcester, MA 01605, USA.

Samantha K Lindberg (SK)

Department of Chemistry and Biochemistry, Worcester Polytechnic Institute, 100 Institute Rd., Worcester, MA 01605, USA.

Karin Plante (K)

Department of Chemistry and Biochemistry, Worcester Polytechnic Institute, 100 Institute Rd., Worcester, MA 01605, USA.

Alonzo H Ross (AH)

Department of Chemistry and Biochemistry, Worcester Polytechnic Institute, 100 Institute Rd., Worcester, MA 01605, USA; Department of Biochemistry and Molecular Pharmacology University of Massachusetts Medical School, Worcester, MA, 01605, USA.

Arne Gericke (A)

Department of Chemistry and Biochemistry, Worcester Polytechnic Institute, 100 Institute Rd., Worcester, MA 01605, USA.

Suzanne Scarlata (S)

Department of Chemistry and Biochemistry, Worcester Polytechnic Institute, 100 Institute Rd., Worcester, MA 01605, USA. Electronic address: sfscarlata@wpi.edu.

Classifications MeSH