Investigation of γ-polyglutamic acid production via asynchronous saccharification and fermentation of raw corn starch.
Polyglutamic Acid
/ analogs & derivatives
Starch
/ metabolism
Fermentation
Bacillus subtilis
/ metabolism
alpha-Amylases
/ metabolism
Glucan 1,4-alpha-Glucosidase
/ metabolism
Zea mays
/ metabolism
Temperature
Maltose
/ metabolism
Glucose
/ metabolism
Bioreactors
/ microbiology
Oligosaccharides
/ metabolism
Industrial Microbiology
/ methods
Components of saccharification liquid
Fermentation
Raw corn starch
Saccharification
γ-polyglutamic acid
Journal
World journal of microbiology & biotechnology
ISSN: 1573-0972
Titre abrégé: World J Microbiol Biotechnol
Pays: Germany
ID NLM: 9012472
Informations de publication
Date de publication:
03 Oct 2024
03 Oct 2024
Historique:
received:
30
07
2024
accepted:
11
09
2024
medline:
3
10
2024
pubmed:
3
10
2024
entrez:
2
10
2024
Statut:
epublish
Résumé
Starch, a crucial raw material, has been extensively investigated for biotechnological applications. However, its application in γ-polyglutamic acid (γ-PGA) production remains unexplored. Based on γ-PGA output of Bacillus subtilis SCP010-1, a novel asynchronous saccharification and fermentation process for γ-PGA synthesis was implemented. The results revealed that a starch concentration of 20%, α-amylase dosage of 75 U/g, liquefaction temperature of 72℃, and γ-PGA yield of 36.31 g/L was achieved. At a glucoamylase dosage of 100 U/g, saccharification 38 h at 60℃, the yield of γ-PGA increased to 48.88 g/L. The contents of total sugar, glucose, maltose and oligosaccharide in saccharified liquid were determined. Through batch fermentation of saccharified liquid in fermentor, the γ-PGA output was elevated to 116.08 g/L. This study can offer a potential cost reduction of 40%, which can be a promising advancement in industrial γ-PGA production. Moreover, our approach can be applied in other starch-based fermentation industries.
Identifiants
pubmed: 39358620
doi: 10.1007/s11274-024-04141-5
pii: 10.1007/s11274-024-04141-5
doi:
Substances chimiques
Polyglutamic Acid
25513-46-6
Starch
9005-25-8
alpha-Amylases
EC 3.2.1.1
poly(gamma-glutamic acid)
0
Glucan 1,4-alpha-Glucosidase
EC 3.2.1.3
Maltose
69-79-4
Glucose
IY9XDZ35W2
Oligosaccharides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
338Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Nature B.V.
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