Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials via authentic standards.

Humans Ceramides / blood Reference Standards Calibration Laboratories / standards Mass Spectrometry / methods Lipidomics / methods Reproducibility of Results

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
03 Oct 2024
Historique:
received: 26 11 2023
accepted: 27 08 2024
medline: 4 10 2024
pubmed: 4 10 2024
entrez: 3 10 2024
Statut: epublish

Résumé

In this community effort, we compare measurements between 34 laboratories from 19 countries, utilizing mixtures of labelled authentic synthetic standards, to quantify by mass spectrometry four clinically used ceramide species in the NIST (National Institute of Standards and Technology) human blood plasma Standard Reference Material (SRM) 1950, as well as a set of candidate plasma reference materials (RM 8231). Participants either utilized a provided validated method and/or their method of choice. Mean concentration values, and intra- and inter-laboratory coefficients of variation (CV) were calculated using single-point and multi-point calibrations, respectively. These results are the most precise (intra-laboratory CVs ≤ 4.2%) and concordant (inter-laboratory CVs < 14%) community-derived absolute concentration values reported to date for four clinically used ceramides in the commonly analyzed SRM 1950. We demonstrate that calibration using authentic labelled standards dramatically reduces data variability. Furthermore, we show how the use of shared RM can correct systematic quantitative biases and help in harmonizing lipidomics. Collectively, the results from the present study provide a significant knowledge base for translation of lipidomic technologies to future clinical applications that might require the determination of reference intervals (RIs) in various human populations or might need to estimate reference change values (RCV), when analytical variability is a key factor for recall during multiple testing of individuals.

Identifiants

DOI: 10.1038/s41467-024-52087-x PMID: 39362843
pubmed: 39362843
doi: 10.1038/s41467-024-52087-x
pii: 10.1038/s41467-024-52087-x
doi:

Substances chimiques

Ceramides 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

8562

Informations de copyright

© 2024. The Author(s).

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Auteurs

Federico Torta (F)

Singapore Lipidomics Incubator, Life Sciences Institute, National University of Singapore, Singapore, 117456, Singapore.
Precision Medicine Translational Research Programme and Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 119077, Singapore.
Signature Research Program in Cardiovascular and Metabolic Disorders, Duke-National University of Singapore (NUS) Medical School, Singapore, 169857, Singapore.

Nils Hoffmann (N)

Institute for Bio- and Geosciences (IBG-5), Forschungszentrum Jülich GmbH, 52428, Jülich, Germany.
Department of Analytical Chemistry, University of Vienna, Vienna, Austria.

Bo Burla (B)

Singapore Lipidomics Incubator, Life Sciences Institute, National University of Singapore, Singapore, 117456, Singapore.
ORCID: 0000-0002-5918-3249

Irina Alecu (I)

Neural Regeneration Laboratory, Ottawa Institute of Systems Biology, Ottawa Brain and Mind Research Institute, Department of Biochemistry, Microbiology, and Immunology, and Department of Chemistry, Centre for Catalysis Research and Innovation, University of Ottawa, Ottawa, K1H 8M5, Canada.

Makoto Arita (M)

RIKEN Center for Integrative Medical Sciences, Yokohama, Japan.
Graduate School of Medical Life Science, Yokohama City University, Yokohama, Japan.
ORCID: 0000-0001-9902-0463

Takeshi Bamba (T)

Division of Metabolomics Center, Medical Research Center for High Depth Omics, Medical Institute of Bioregulation, Kyushu University, 3‑1‑1, Maidashi, Higashi‑ku, Fukuoka, 812‑8582, Japan.
ORCID: 0000-0002-7442-2738

Steffany A L Bennett (SAL)

Neural Regeneration Laboratory, Ottawa Institute of Systems Biology, Ottawa Brain and Mind Research Institute, Department of Biochemistry, Microbiology, and Immunology, and Department of Chemistry, Centre for Catalysis Research and Innovation, University of Ottawa, Ottawa, K1H 8M5, Canada.
ORCID: 0000-0001-7944-5800

Justine Bertrand-Michel (J)

MetaboHUBMetaToul Facility, I2MC U1297 Inserm, Toulouse, France.

Britta Brügger (B)

Heidelberg University Biochemistry Center (BZH), Im Neuenheimer Feld 328, 69120, Heidelberg, Germany.
ORCID: 0000-0002-3477-8270

Mónica P Cala (MP)

Metabolomics Core Facility-MetCore, Universidad de los Andes, Bogotá, 111711, Colombia.

Dolores Camacho-Muñoz (D)

Laboratory for Lipidomics and Lipid Biology, Division of Pharmacy and Optometry, School of Health Sciences, Faculty of Biology Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Manchester, M13 9NT, United Kingdom.

Antonio Checa (A)

Unit of Integrative Metabolomics, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.

Michael Chen (M)

Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.

Michaela Chocholoušková (M)

Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10, Pardubice, Czech Republic.

Michelle Cinel (M)

Baker Heart and Diabetes Institute, Melbourne, VIC, 3004, Australia.
Baker Department of Cardiovascular Research, Translation and Implementation, La Trobe University, Bundoora, VIC, 3086, Australia.

Emeline Chu-Van (E)

Université Paris-Saclay, CEA, INRAE, Département Médicaments et Technologies pour la Santé (DMTS), MetaboHUB, F-91191, Gif sur Yvette, France.

Benoit Colsch (B)

Université Paris-Saclay, CEA, INRAE, Département Médicaments et Technologies pour la Santé (DMTS), MetaboHUB, F-91191, Gif sur Yvette, France.
ORCID: 0000-0002-8023-504X

Cristina Coman (C)

Department of Analytical Chemistry, University of Vienna, Vienna, Austria.

Lisa Connell (L)

Avanti Polar Lipids, Alabaster, AL, USA.

Bebiana C Sousa (BC)

Babraham Institute, Babraham Research Campus, Cambridge, MA, CB22 3AT, USA.

Alex M Dickens (AM)

Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520, Turku, Finland.
Department of Chemistry, University of Turku, Turku, Finland.
ORCID: 0000-0002-3178-8449

Maria Fedorova (M)

Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, University of Leipzig, 04013, Leipzig, Germany.
Center for Biotechnology and Biomedicine, University of Leipzig, 04013, Leipzig, Germany.
Center of Membrane Biochemistry and Lipid Research, Faculty of Medicine Carl Gustav Carus of TU Dresden, 01307, Dresden, Germany.
ORCID: 0000-0002-4692-3885

Finnur Freyr Eiríksson (FF)

Faculty of Pharmaceutical Sciences, University of Iceland, Reykjavik, Iceland.
ArcticMass, Reykjavik, Iceland.

Hector Gallart-Ayala (H)

Metabolomics Platform, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.
ORCID: 0000-0003-2333-0646

Mohan Ghorasaini (M)

Center for Proteomics and Metabolomics, Leiden University Medical Center, 2333ZA, Leiden, The Netherlands.

Martin Giera (M)

Center for Proteomics and Metabolomics, Leiden University Medical Center, 2333ZA, Leiden, The Netherlands.

Xue Li Guan (XL)

Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, 636921, Singapore.
ORCID: 0000-0002-5189-8178

Mark Haid (M)

Metabolomics and Proteomics Core, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, 85764, Germany.
ORCID: 0000-0001-6118-1333

Thomas Hankemeier (T)

Metabolomics and Analytics Centre, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands.

Amy Harms (A)

Metabolomics and Analytics Centre, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands.
ORCID: 0000-0002-2931-4295

Marcus Höring (M)

University Hospital of Regensburg, Institute of Clinical Chemistry and Laboratory Medicine, Franz-Josef-Strauß-Allee 11, 93053, Regensburg, Germany.

Michal Holčapek (M)

Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10, Pardubice, Czech Republic.
ORCID: 0000-0003-3978-1249

Thorsten Hornemann (T)

Institute of Clinical Chemistry, University Zurich, 8952, Schlieren, Switzerland.
ORCID: 0000-0002-4218-1087

Chunxiu Hu (C)

State Key Laboratory of Medical Proteomics, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.

Andreas J Hülsmeier (AJ)

Institute of Clinical Chemistry, University Zurich, 8952, Schlieren, Switzerland.
ORCID: 0000-0001-6987-8979

Kevin Huynh (K)

Baker Heart and Diabetes Institute, Melbourne, VIC, 3004, Australia.
Baker Department of Cardiovascular Research, Translation and Implementation, La Trobe University, Bundoora, VIC, 3086, Australia.
ORCID: 0000-0001-6170-2207

Christina M Jones (CM)

Chemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD, 20899, USA.

Julijana Ivanisevic (J)

Metabolomics Platform, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.
ORCID: 0000-0001-8267-2705

Yoshihiro Izumi (Y)

Division of Metabolomics Center, Medical Research Center for High Depth Omics, Medical Institute of Bioregulation, Kyushu University, 3‑1‑1, Maidashi, Higashi‑ku, Fukuoka, 812‑8582, Japan.
ORCID: 0000-0003-4608-4652

Harald C Köfeler (HC)

Core Facility Mass Spectrometry, Medical University of Graz, 8010, Graz, Austria.
ORCID: 0000-0002-2725-9616

Sin Man Lam (SM)

LipidALL Technologies, Changzhou, 213000, Jiangshu, China.
State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
ORCID: 0000-0001-9037-904X

Mike Lange (M)

Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, University of Leipzig, 04013, Leipzig, Germany.
Center for Biotechnology and Biomedicine, University of Leipzig, 04013, Leipzig, Germany.

Jong Cheol Lee (JC)

Department of Chemistry, Yonsei University, Seoul, 03722, South Korea.

Gerhard Liebisch (G)

University Hospital of Regensburg, Institute of Clinical Chemistry and Laboratory Medicine, Franz-Josef-Strauß-Allee 11, 93053, Regensburg, Germany.
ORCID: 0000-0003-4886-0811

Katrice Lippa (K)

Chemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD, 20899, USA.

Andrea F Lopez-Clavijo (AF)

Babraham Institute, Babraham Research Campus, Cambridge, MA, CB22 3AT, USA.
ORCID: 0000-0002-6249-7767

Malena Manzi (M)

Centro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2390, C1425FQD, Ciudad de Buenos Aires, Argentina.
Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes, 2160 C1428EGA, Buenos Aires, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Departamento de Desarrollo Analítico y Control de Procesos, Instituto Nacional de Tecnología Industrial, Av. General Paz 5445, B1650WAB, Buenos Aires, Argentina.

Manuela R Martinefski (MR)

Centro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2390, C1425FQD, Ciudad de Buenos Aires, Argentina.
Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Ciencias Químicas, Buenos Aires, Junin 954, Junin, C1113AAD, CABA, Argentina.
ORCID: 0000-0002-4501-3783

Raviswamy G H Math (RGH)

National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, Karnataka, 560065, India.

Satyajit Mayor (S)

National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, Karnataka, 560065, India.
ORCID: 0000-0001-9842-6963

Peter J Meikle (PJ)

Baker Heart and Diabetes Institute, Melbourne, VIC, 3004, Australia.
Baker Department of Cardiovascular Research, Translation and Implementation, La Trobe University, Bundoora, VIC, 3086, Australia.
ORCID: 0000-0002-2593-4665

María Eugenia Monge (ME)

Centro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2390, C1425FQD, Ciudad de Buenos Aires, Argentina.

Myeong Hee Moon (MH)

Department of Chemistry, Yonsei University, Seoul, 03722, South Korea.
ORCID: 0000-0002-5454-2601

Sneha Muralidharan (S)

Singapore Lipidomics Incubator, Life Sciences Institute, National University of Singapore, Singapore, 117456, Singapore.
National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, Karnataka, 560065, India.

Anna Nicolaou (A)

Laboratory for Lipidomics and Lipid Biology, Division of Pharmacy and Optometry, School of Health Sciences, Faculty of Biology Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Manchester, M13 9NT, United Kingdom.
Lydia Becker Institute of Immunology and Inflammation; Faculty of Biology, Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Manchester, M13 9NT, United Kingdom.
ORCID: 0000-0002-1314-413X

Thao Nguyen-Tran (T)

Neural Regeneration Laboratory, Ottawa Institute of Systems Biology, Ottawa Brain and Mind Research Institute, Department of Biochemistry, Microbiology, and Immunology, and Department of Chemistry, Centre for Catalysis Research and Innovation, University of Ottawa, Ottawa, K1H 8M5, Canada.
ORCID: 0000-0002-5507-618X

Valerie B O'Donnell (VB)

Systems Immunity Research Institute, School of Medicine, Cardiff University, Heath Park, Cardiff, CF14 4XN, UK.
ORCID: 0000-0003-4089-8460

Matej Orešič (M)

Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520, Turku, Finland.
School of Medical Sciences, Faculty of Medicine and Health, Örebro University, 702 81, Örebro, Sweden.
ORCID: 0000-0002-2856-9165

Arvind Ramanathan (A)

Institute for Stem Cell Science and Regenerative Medicine, 560065, Bangalore, India.
ORCID: 0000-0002-0375-3740

Fabien Riols (F)

Metabolomics and Proteomics Core, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, 85764, Germany.

Daisuke Saigusa (D)

Laboratory of Biomedical and Analytical Sciences, Faculty of Pharma-Science, Teikyo University, 2-11-1 Kaga, Itabashi-ku, Tokyo, 173-8605, Japan.
Department of Integrative Genomics, Tohoku Medical Megabank Organization, Tohoku University, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8573, Japan.

Tracey B Schock (TB)

Chemical Science Division, National Institute of Standards and Technology, Charleston, SC, 29412, USA.
ORCID: 0000-0002-1808-7816

Heidi Schwartz-Zimmermann (H)

Christian Doppler Laboratory for Innovative Gut Health Concepts of Livestock, Institute of Bioanalytics and Agro-Metabolomics, Department of Agrobiotechnology (IFATulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Konrad-Lorenz-Str. 20, 3430, Tulln, Austria.

Guanghou Shui (G)

State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
ORCID: 0000-0002-1621-9643

Madhulika Singh (M)

Metabolomics and Analytics Centre, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands.
ORCID: 0000-0002-7433-9352

Masatomo Takahashi (M)

Division of Metabolomics Center, Medical Research Center for High Depth Omics, Medical Institute of Bioregulation, Kyushu University, 3‑1‑1, Maidashi, Higashi‑ku, Fukuoka, 812‑8582, Japan.
ORCID: 0000-0002-2493-2684

Margrét Thorsteinsdóttir (M)

Faculty of Pharmaceutical Sciences, University of Iceland, Reykjavik, Iceland.
ArcticMass, Reykjavik, Iceland.

Noriyuki Tomiyasu (N)

Division of Metabolomics Center, Medical Research Center for High Depth Omics, Medical Institute of Bioregulation, Kyushu University, 3‑1‑1, Maidashi, Higashi‑ku, Fukuoka, 812‑8582, Japan.

Anthony Tournadre (A)

MetaboHUBMetaToul Facility, I2MC U1297 Inserm, Toulouse, France.

Hiroshi Tsugawa (H)

RIKEN Center for Integrative Medical Sciences, Yokohama, Japan.
Graduate School of Medical Life Science, Yokohama City University, Yokohama, Japan.
RIKEN Center for Sustainable Resource Science, Yokohama, Japan.
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
ORCID: 0000-0002-2015-3958

Victoria J Tyrrell (VJ)

Systems Immunity Research Institute, School of Medicine, Cardiff University, Heath Park, Cardiff, CF14 4XN, UK.

Grace van der Gugten (G)

St. Paul's Hospital, Department of Pathology and Laboratory Medicine, Vancouver, BC, Canada.

Michael O Wakelam (MO)

Babraham Institute, Babraham Research Campus, Cambridge, MA, CB22 3AT, USA.

Craig E Wheelock (CE)

Unit of Integrative Metabolomics, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.
Department of Respiratory Medicine and Allergy, Karolinska University Hospital, Stockholm, Sweden.
ORCID: 0000-0002-8113-0653

Denise Wolrab (D)

Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10, Pardubice, Czech Republic.

Guowang Xu (G)

State Key Laboratory of Medical Proteomics, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
ORCID: 0000-0003-4298-3554

Tianrun Xu (T)

State Key Laboratory of Medical Proteomics, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.

John A Bowden (JA)

Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, 32610, USA.

Kim Ekroos (K)

Lipidomics Consulting Ltd., Espoo, Finland. kim@lipidomicsconsulting.com.
ORCID: 0000-0001-6810-4736

Robert Ahrends (R)

Department of Analytical Chemistry, University of Vienna, Vienna, Austria. robert.ahrends@univie.ac.at.
ORCID: 0000-0003-0232-3375

Markus R Wenk (MR)

Singapore Lipidomics Incubator, Life Sciences Institute, National University of Singapore, Singapore, 117456, Singapore. mwenk@hbku.edu.qa.
Precision Medicine Translational Research Programme and Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 119077, Singapore. mwenk@hbku.edu.qa.
College of Health and Life Sciences, Hamad Bin Khalifa University, Doha, Qatar. mwenk@hbku.edu.qa.
ORCID: 0000-0001-5447-7881

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Concordant inter-laboratory derived...
questionsmedicales.fr › Lipides › Lipides membranaires › Sphingolipides › Glycosphingolipides › Glycosphingolipides neutres › Céramides › Concordant inter-laboratory derived...
questionsmedicales.fr › Techniques d'investigation › Poids et mesures › Normes de référence › Concordant inter-laboratory derived...
questionsmedicales.fr › Techniques d'investigation › Poids et mesures › Calibrage › Concordant inter-laboratory derived...
questionsmedicales.fr › Établissements, main d'oeuvre et services de soins de santé › Établissements de santé › Laboratoires › Concordant inter-laboratory derived...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Concordant inter-laboratory derived...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Métabolomique › Lipidomique › Concordant inter-laboratory derived...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Méthodologie en recherche épidémiologique › Reproductibilité des résultats › Concordant inter-laboratory derived...