The Buds of Oscarella lobularis (Porifera, Homoscleromorpha): A New Convenient Model for Sponge Cell and Evolutionary Developmental Biology.
culture
imaging
morphogenesis
regeneration
reproduction
staining
Journal
Journal of experimental zoology. Part B, Molecular and developmental evolution
ISSN: 1552-5015
Titre abrégé: J Exp Zool B Mol Dev Evol
Pays: United States
ID NLM: 101168228
Informations de publication
Date de publication:
04 Oct 2024
04 Oct 2024
Historique:
revised:
31
05
2024
received:
17
01
2024
accepted:
24
07
2024
medline:
4
10
2024
pubmed:
4
10
2024
entrez:
4
10
2024
Statut:
aheadofprint
Résumé
The comparative study of the four non-bilaterian phyla (Cnidaria, Placozoa, Ctenophora, and Porifera) provides insights into the origin of bilaterian traits. To complete our knowledge of the cell biology and development of these animals, additional non-bilaterian models are needed. Given the developmental, histological, ecological, and genomic differences between the four sponge classes (Demospongiae, Calcarea, Homoscleromorpha, and Hexactinellida), we have been developing the Oscarella lobularis (Porifera, class Homoscleromorpha) model over the past 15 years. Here, we report a new step forward by inducing, producing, and maintaining in vitro thousands of clonal buds that now make possible various downstream applications. This study provides a full description of bud morphology, physiology, cells and tissues, from their formation to their development into juveniles, using adapted cell staining protocols. In addition, we show that buds have outstanding capabilities of regeneration after being injured and of re-epithelization after complete cell dissociation. Altogether, Oscarella buds constitute a relevant all-in-one sponge model to access a large set of biological processes, including somatic morphogenesis, epithelial morphogenesis, cell fate, body axes formation, nutrition, contraction, ciliary beating, and respiration.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : This work was funded by the Centre National de la recherche Scientifique (CNRS, UMR7263 and UMR7288) : project for international scientific cooperation (PICS) STraS involving CR, AE, SC, ER, CB, ELG, ALB, DMH, CM, AV), and also by the Aix-Marseille University and the A*MIDEX foundation project (ANR-11-IDEX-0001-02 to CB, ER, ALB, CR, NS, SC, ChM, AE;
Organisme : AMX-18-INT-021 to CB, ER, ALB, CR, DML, NB, CM); as well as the National research agency (ANR) : ANR-21-CE13-0013-02 to ALB, DML, CB, ER, CR, CM, SC and ANR-22-CE13-0026 to DS, JJ, ER, CB, QS, CR, CM, SC); ALB, DMH and NB are supported by the LabEx INFORM (ANR-11-LABX-0054) both funded by the «Investissements d'Avenir » French Government program, managed by the French National Research Agency (ANR).
Organisme : The DB RAS government basic research program no. 0088-2021-0009 (TEM studies) to AE. AE also acknowledge the Saint-Petersburg State University (Saint-Petersburg, Russia) and the Koltzov Institute of Developmental Biology of Russian Academy of Sciences (Moscow, Russia) for their technical and financial support to perform some of the experiments.
Organisme : The region Sud/PACA and Aix-Marseille University are also acknowledged for funding PhD fellowships of Laura Fierro-Constaín and Amélie Vernale, respectively. The light and electron microscopy experiments were performed at the PiCSL-FBI core facility (IBDM, AMU-Marseille), a member of the France-BioImaging National Research Infrastructure (ANR-10-INBS-04).
Informations de copyright
© 2024 The Author(s). Journal of Experimental Zoology Part B: Molecular and Developmental Evolution published by Wiley Periodicals LLC.
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