Comprehensive stable-isotope tracing of glucose and amino acids identifies metabolic by-products and their sources in CHO cell culture.

Animals CHO Cells Cricetulus Glucose / metabolism Amino Acids / metabolism Carbon Isotopes / metabolism Gas Chromatography-Mass Spectrometry / methods Cell Culture Techniques / methods Cricetinae Immunoglobulin G / metabolism Isotope Labeling / methods
amino acid catabolism mammalian cell culture mass spectrometry metabolic by-products stable isotope labeling

Journal

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876

Informations de publication

Date de publication:
08 Oct 2024
Historique:
medline: 4 10 2024
pubmed: 4 10 2024
entrez: 4 10 2024
Statut: ppublish

Résumé

Mammalian cell culture processes are widely utilized for biotherapeutics production, disease diagnostics, and biosensors, and hence, should be optimized to support robust cell growth and viability. However, toxic by-products accumulate in cultures due to inefficiencies in metabolic activities and nutrient utilization. In this study, we applied comprehensive

Identifiants

DOI: 10.1073/pnas.2403033121 PMID: 39365816
pubmed: 39365816
doi: 10.1073/pnas.2403033121
doi:

Substances chimiques

Glucose IY9XDZ35W2
Amino Acids 0
Carbon Isotopes 0
Immunoglobulin G 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2403033121

Subventions

Organisme : NSF (NSF)
ID : 1624684

Déclaration de conflit d'intérêts

Competing interests statement:Current employment of authors: J.E.G. is currently affiliated with Takeda Pharmaceutical Company; H.M.N. is currently affiliated with IQVIA; E.H.O. is currently affiliated with Genentech; V.G.D. is currently affiliated with Pfizer; B.O.M. is currently affiliated with Amgen; S.K. is currently affiliated with Sanofi.

Auteurs

Jacqueline E Gonzalez (JE)

Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716.

Harnish Mukesh Naik (HM)

Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218.
ORCID: 0000-0002-8303-4219

Eleanor H Oates (EH)

Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716.
ORCID: 0000-0002-5382-6557

Venkata Gayatri Dhara (VG)

Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218.

Brian O McConnell (BO)

Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716.

Swetha Kumar (S)

Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218.

Michael J Betenbaugh (MJ)

Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218.

Maciek R Antoniewicz (MR)

Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716.
Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109.
ORCID: 0000-0002-0087-2875

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Comprehensive stable-isotope tracing of...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules CHO › Comprehensive stable-isotope tracing of...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Arvicolinae › Cricetinae › Cricetulus › Comprehensive stable-isotope tracing of...
questionsmedicales.fr › Glucides › Sucres › Oses › Hexose › Glucose › Comprehensive stable-isotope tracing of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Comprehensive stable-isotope tracing of...
questionsmedicales.fr › Produits chimiques inorganiques › Isotopes › Isotopes du carbone › Comprehensive stable-isotope tracing of...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Chromatographie gazeuse-spectrométrie de masse › Comprehensive stable-isotope tracing of...
questionsmedicales.fr › Techniques d'investigation › Techniques in vitro › Techniques de culture › Techniques de culture cellulaire › Comprehensive stable-isotope tracing of...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Arvicolinae › Cricetinae › Comprehensive stable-isotope tracing of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Isotypes des immunoglobulines › Immunoglobuline G › Comprehensive stable-isotope tracing of...
questionsmedicales.fr › Techniques d'investigation › Marquage isotopique › Comprehensive stable-isotope tracing of...