Self-assembling nanoparticle engineered from the ferritinophagy complex as a rabies virus vaccine candidate.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
04 Oct 2024
Historique:
received: 17 03 2024
accepted: 24 09 2024
medline: 5 10 2024
pubmed: 5 10 2024
entrez: 4 10 2024
Statut: epublish

Résumé

Over the past decade, there has been a growing interest in ferritin-based vaccines due to their enhanced antigen immunogenicity and favorable safety profiles, with several vaccine candidates targeting various pathogens advancing to phase I clinical trials. Nevertheless, challenges associated with particle heterogeneity, improper assembly and unanticipated immunogenicity due to the bulky protein adaptor have impeded further advancement. To overcome these challenges, we devise a universal ferritin-adaptor delivery platform based on structural insights derived from the natural ferritinophagy complex of the human ferritin heavy chain (FTH1) and the nuclear receptor coactivator 4 (NCOA4). The engineered ferritinophagy (Fagy)-tag peptide demonstrate significantly enhanced binding affinity to the 24-mer ferritin nanoparticle, enabling efficient antigen presentation. Subsequently, we construct a self-assembling rabies virus (RABV) vaccine candidate by noncovalently conjugating the Fagy-tagged glycoprotein domain III (G

Identifiants

pubmed: 39366932
doi: 10.1038/s41467-024-52908-z
pii: 10.1038/s41467-024-52908-z
doi:

Substances chimiques

Apoferritins 9013-31-4
Rabies Vaccines 0
Ferritins 9007-73-2
FTH1 protein, human EC 1.-
Antibodies, Viral 0
Antigens, Viral 0
Oxidoreductases EC 1.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

8601

Informations de copyright

© 2024. The Author(s).

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Auteurs

Dan Fu (D)

State Key Laboratory of Medicinal Chemical Biology and College of Life Sciences, Nankai University, Tianjin, PR China.
College of Pharmacy, Nankai University, Tianjin, PR China.
Guangzhou Laboratory, Guangzhou, Guangdong, PR China.

Wenming Wang (W)

Key Laboratory of Chemical Biology and Molecular Engineering of the Education Ministry, Institute of Molecular Science, Shanxi University, Taiyuan, PR China.

Yan Zhang (Y)

School of Public Health, Beihua University, Jilin, PR China.

Fan Zhang (F)

National Facility for Translational Medicine (Beijing), Medical Innovation Research Division, PLA General Hospital, Beijing, PR China.
Department of Oncology, The Fifth Medical Center, PLA General Hospital, Beijing, PR China.

Pinyi Yang (P)

State Key Laboratory of Medicinal Chemical Biology and College of Life Sciences, Nankai University, Tianjin, PR China.

Chun Yang (C)

College of Basic Medicine, Beihua University, Jilin, PR China.

Yufei Tian (Y)

Changchun Veterinary Research Institute (CVRI), Chinese Academy of Agricultural Sciences (CAAS), Jingyue Economic Development Zone, Changchun, PR China.

Renqi Yao (R)

National Facility for Translational Medicine (Beijing), Medical Innovation Research Division, PLA General Hospital, Beijing, PR China.

Jingwu Jian (J)

State Key Laboratory of Medicinal Chemical Biology and College of Life Sciences, Nankai University, Tianjin, PR China.

Zixian Sun (Z)

Guangzhou Laboratory, Guangzhou, Guangdong, PR China.

Nan Zhang (N)

State Key Laboratory of Medicinal Chemical Biology and College of Life Sciences, Nankai University, Tianjin, PR China.
Central Laboratory, Hebei Collaborative Innovation Center of Tumor Microecological Metabolism Regulation, Affiliated Hospital of Hebei University, Baoding, Hebei, PR China.

Zhiyu Ni (Z)

Central Laboratory, Hebei Collaborative Innovation Center of Tumor Microecological Metabolism Regulation, Affiliated Hospital of Hebei University, Baoding, Hebei, PR China.

Zihe Rao (Z)

State Key Laboratory of Medicinal Chemical Biology and College of Life Sciences, Nankai University, Tianjin, PR China. raozh@mail.tsinghua.edu.cn.

Lei Zhao (L)

National Facility for Translational Medicine (Beijing), Medical Innovation Research Division, PLA General Hospital, Beijing, PR China. jackyzhao010@126.com.
Department of Oncology, The Fifth Medical Center, PLA General Hospital, Beijing, PR China. jackyzhao010@126.com.

Yu Guo (Y)

State Key Laboratory of Medicinal Chemical Biology and College of Life Sciences, Nankai University, Tianjin, PR China. guoyu@nankai.edu.cn.
Guangzhou Laboratory, Guangzhou, Guangdong, PR China. guoyu@nankai.edu.cn.

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