Melatonin decreases excessive polyspermy for single oocyte in pigs through the MT2 receptor.
Animals
Melatonin
/ pharmacology
Oocytes
/ drug effects
Swine
Fertilization in Vitro
/ methods
Female
Receptor, Melatonin, MT2
/ metabolism
In Vitro Oocyte Maturation Techniques
/ methods
Embryonic Development
/ drug effects
Fertilization
/ drug effects
Blastocyst
/ drug effects
Tryptamines
/ pharmacology
Melatonin
Oocyte
Organelles
Pig
Polyspermy
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
05 Oct 2024
05 Oct 2024
Historique:
received:
23
05
2024
accepted:
30
09
2024
medline:
5
10
2024
pubmed:
5
10
2024
entrez:
4
10
2024
Statut:
epublish
Résumé
Melatonin supplementation during in vitro maturation (IVM) improves porcine oocyte maturation and embryonic development by exerting antioxidative effects. Nevertheless, the mechanism by which melatonin prevents polyspermy after in vitro fertilization (IVF) remains unclear. Here, we examined the effects of melatonin on cytoplasmic maturation and the incidence of polyspermic penetration in porcine oocytes. No statistically significant difference was observed in the rate of first polar body formation between the groups (Control, Melatonin, Melatonin + Luzindole, and Melatonin + 4-P-PDOT). Interestingly, melatonin supplementation significantly improved the cytoplasmic maturation of porcine oocytes by enhancing the normal distribution of organelles (Golgi apparatus, endoplasmic reticulum and mitochondria) and upregulating organelle-related gene expressions (P < 0.05). However, these promotional effects were counteracted by melatonin antagonists, suggesting that melatonin enhances cytoplasmic maturation through its receptors in porcine oocytes. Melatonin supplementation also significantly improved the rate of diploid and blastocyst formation after IVF by promoting the normal distribution of cortical granules (P < 0.05). In conclusion, melatonin supplementation during in vitro maturation of porcine oocyte improves fertilization efficiency and embryonic developmental competence by enhancing cytoplasmic maturation.
Identifiants
pubmed: 39367161
doi: 10.1038/s41598-024-74969-2
pii: 10.1038/s41598-024-74969-2
doi:
Substances chimiques
Melatonin
JL5DK93RCL
Receptor, Melatonin, MT2
0
luzindole
117946-91-5
Tryptamines
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
23153Subventions
Organisme : Self-financing Project of Harbin Science and Technology Plan
ID : 2023ZCZJCG013
Organisme : National Key Research and Development Program of China
ID : 2022YFD1302203
Organisme : National Key Research and Development Program of China
ID : 2021YFA0805902
Organisme : National Natural Science Foundation of China
ID : 32372884
Informations de copyright
© 2024. The Author(s).
Références
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