SENP1 mediates zinc-induced ZnT6 deSUMOylation at Lys-409 involved in the regulation of zinc metabolism in Golgi apparatus.
Zinc
/ metabolism
Golgi Apparatus
/ metabolism
Animals
Sumoylation
Cysteine Endopeptidases
/ metabolism
Cation Transport Proteins
/ metabolism
Humans
Mice
Transcription Factor MTF-1
Male
SUMO-1 Protein
/ metabolism
DNA-Binding Proteins
/ metabolism
Liver
/ metabolism
Mice, Inbred C57BL
Transcription Factors
/ metabolism
Protein Processing, Post-Translational
Carrier Proteins
MTF-1
SENP1
SUMOylation
Zinc
Zinc transporter
Zn homeostasis
Journal
Cellular and molecular life sciences : CMLS
ISSN: 1420-9071
Titre abrégé: Cell Mol Life Sci
Pays: Switzerland
ID NLM: 9705402
Informations de publication
Date de publication:
05 Oct 2024
05 Oct 2024
Historique:
received:
01
07
2024
accepted:
16
09
2024
revised:
10
09
2024
medline:
6
10
2024
pubmed:
6
10
2024
entrez:
5
10
2024
Statut:
epublish
Résumé
Zinc (Zn) transporters contribute to the maintenance of intracellular Zn homeostasis in vertebrate, whose activity and function are modulated by post-translational modification. However, the function of small ubiquitin-like modifier (SUMOylation) in Zn metabolism remains elusive. Here, compared with low Zn group, a high-Zn diet significantly increases hepatic Zn content and upregulates the expression of metal-response element-binding transcription factor-1 (MTF-1), Zn transporter 6 (ZnT6) and deSUMOylation enzymes (SENP1, SENP2, and SENP6), but inhibits the expression of SUMO proteins and the E1, E2, and E3 enzymes. Mechanistically, Zn triggers the activation of the MTF-1/SENP1 pathway, resulting in the reduction of ZnT6 SUMOylation at Lys 409 by small ubiquitin-like modifier 1 (SUMO1), and promoting the deSUMOylation process mediated by SENP1. SUMOylation modification of ZnT6 has no influence on its localization but reduces its protein stability. Importantly, deSUMOylation of ZnT6 is crucial for controlling Zn export from the cytosols into the Golgi apparatus. In conclusion, for the first time, we elucidate a novel mechanism by which SUMO1-catalyzed SUMOylation and SENP1-mediated deSUMOylation of ZnT6 orchestrate the regulation of Zn metabolism within the Golgi apparatus.
Identifiants
pubmed: 39367979
doi: 10.1007/s00018-024-05452-4
pii: 10.1007/s00018-024-05452-4
doi:
Substances chimiques
Zinc
J41CSQ7QDS
Cysteine Endopeptidases
EC 3.4.22.-
Cation Transport Proteins
0
Transcription Factor MTF-1
0
Senp1 protein, mouse
EC 3.4.22.-
SUMO-1 Protein
0
DNA-Binding Proteins
0
Transcription Factors
0
zinc-binding protein
0
Carrier Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
422Subventions
Organisme : National Natural Science Foundation of China
ID : 32030111 and 31872585
Informations de copyright
© 2024. The Author(s).
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