Interferon regulatory factor 7 alleviates the experimental colitis through enhancing IL-28A-mediated intestinal epithelial integrity.


Journal

Journal of translational medicine
ISSN: 1479-5876
Titre abrégé: J Transl Med
Pays: England
ID NLM: 101190741

Informations de publication

Date de publication:
06 Oct 2024
Historique:
received: 02 03 2024
accepted: 02 09 2024
medline: 7 10 2024
pubmed: 7 10 2024
entrez: 6 10 2024
Statut: epublish

Résumé

The incidence of inflammatory bowel disease (IBD) is on the rise in developing countries, and investigating the underlying mechanisms of IBD is essential for the development of targeted therapeutic interventions. Interferon regulatory factor 7 (IRF7) is known to exert pro-inflammatory effects in various autoimmune diseases, yet its precise role in the development of colitis remains unclear. We analyzed the clinical significance of IRF7 in ulcerative colitis (UC) by searching RNA-Seq databases and collecting tissue samples from clinical UC patients. And, we performed dextran sodium sulfate (DSS)-induced colitis modeling using WT and Irf7 In this study, we found that IRF7 expression is significantly reduced in patients with UC, and also demonstrated that Irf7 These findings underscore the pivotal role of IRF7 in preserving intestinal homeostasis and forestalling the onset of colitis.

Sections du résumé

BACKGROUND BACKGROUND
The incidence of inflammatory bowel disease (IBD) is on the rise in developing countries, and investigating the underlying mechanisms of IBD is essential for the development of targeted therapeutic interventions. Interferon regulatory factor 7 (IRF7) is known to exert pro-inflammatory effects in various autoimmune diseases, yet its precise role in the development of colitis remains unclear.
METHODS METHODS
We analyzed the clinical significance of IRF7 in ulcerative colitis (UC) by searching RNA-Seq databases and collecting tissue samples from clinical UC patients. And, we performed dextran sodium sulfate (DSS)-induced colitis modeling using WT and Irf7
RESULTS RESULTS
In this study, we found that IRF7 expression is significantly reduced in patients with UC, and also demonstrated that Irf7
CONCLUSION CONCLUSIONS
These findings underscore the pivotal role of IRF7 in preserving intestinal homeostasis and forestalling the onset of colitis.

Identifiants

pubmed: 39370517
doi: 10.1186/s12967-024-05673-y
pii: 10.1186/s12967-024-05673-y
doi:

Substances chimiques

Interferon Regulatory Factor-7 0
Dextran Sulfate 9042-14-2
interferon-lambda protein, mouse 0
Interleukins 0
Cytokines 0
Interferon Lambda 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

905

Subventions

Organisme : National Natural Science Foundation of China
ID : 31960163
Organisme : The Jinggang Scholar Program of Jiangxi Province
ID : QD202205
Organisme : The Scientific and Technological Innovation Team Project and Key Project of Cardiocerebrovascular Disease of Gannan Medical University
ID : TD2021JC01
Organisme : The Scientific and Technological Innovation Team Project and Key Project of Cardiocerebrovascular Disease of Gannan Medical University
ID : XN201906
Organisme : Science and Technology Planning Project of Jiangxi Provincial Health Commission
ID : SKJP_220210670
Organisme : the program of Guangdong Provincial Clinical Research Center for Digestive Diseases
ID : 2020B1111170004

Informations de copyright

© 2024. The Author(s).

Références

Ungaro R, Mehandru S, Allen PB, Peyrin-Biroulet L, Colombel JF. Ulcerative colitis. Lancet. 2017;389(10080):1756–70.
pubmed: 27914657 doi: 10.1016/S0140-6736(16)32126-2
Nowarski R, Jackson R, Gagliani N, de Zoete MR, Palm NW, Bailis W, et al. Epithelial IL-18 equilibrium controls barrier function in colitis. Cell. 2015;163(6):1444–56.
pubmed: 26638073 pmcid: 4943028 doi: 10.1016/j.cell.2015.10.072
Taniguchi K, Karin M. NF-κB, inflammation, immunity and cancer: coming of age. Nat Rev Immunol. 2018;18(5):309–24.
pubmed: 29379212 doi: 10.1038/nri.2017.142
Davé SH, Tilstra JS, Matsuoka K, Li F, Karrasch T, Uno JK, et al. Amelioration of chronic murine colitis by peptide-mediated transduction of the IkappaB kinase inhibitor NEMO binding domain peptide. J Immunol. 2007;179(11):7852–9.
pubmed: 18025231 doi: 10.4049/jimmunol.179.11.7852
Murano M, Maemura K, Hirata I, Toshina K, Nishikawa T, Hamamoto N, et al. Therapeutic effect of intracolonically administered nuclear factor kappa B (p65) antisense oligonucleotide on mouse dextran sulphate sodium (DSS)-induced colitis. Clin Exp Immunol. 2000;120(1):51–8.
pubmed: 10759763 pmcid: 1905625 doi: 10.1046/j.1365-2249.2000.01183.x
Wang S, Lin Y, Yuan X, Li F, Guo L, Wu B. REV-ERBα integrates colon clock with experimental colitis through regulation of NF-κB/NLRP3 axis. Nat Commun. 2018;9(1):4246.
pubmed: 30315268 pmcid: 6185905 doi: 10.1038/s41467-018-06568-5
Honda K, Taniguchi T. IRFs: master regulators of signalling by toll-like receptors and cytosolic pattern-recognition receptors. Nat Rev Immunol. 2006;6(9):644–58.
pubmed: 16932750 doi: 10.1038/nri1900
Farlik M, Rapp B, Marie I, Levy DE, Jamieson AM, Decker T. Contribution of a TANK-binding kinase 1-interferon (IFN) regulatory factor 7 pathway to IFN-γ-induced gene expression. Mol Cell Biol. 2012;32(6):1032–43.
pubmed: 22252317 pmcid: 3295005 doi: 10.1128/MCB.06021-11
Odendall C, Dixit E, Stavru F, Bierne H, Franz KM, Durbin AF, et al. Diverse intracellular pathogens activate type III interferon expression from peroxisomes. Nat Immunol. 2014;15(8):717–26.
pubmed: 24952503 pmcid: 4106986 doi: 10.1038/ni.2915
Baños-Lara Mdel R, Harvey L, Mendoza A, Simms D, Chouljenko VN, Wakamatsu N, et al. Impact and regulation of lambda interferon response in human metapneumovirus infection. J Virol. 2015;89(1):730–42.
pubmed: 25355870 doi: 10.1128/JVI.02897-14
Kotenko SV. IFN-lambdas mediate antiviral protection through a distinct class II cytokine receptor complex. Nat Immunol. 2003;4:69.
pubmed: 12483210 doi: 10.1038/ni875
Li W, Lewis-Antes A, Huang J, Balan M, Kotenko SV. Regulation of apoptosis by type III interferons. Cell Prolif. 2008;41:960.
pubmed: 19040572 pmcid: 6496378 doi: 10.1111/j.1365-2184.2008.00558.x
Maher SG. IFNalpha and IFNlambda differ in their antiproliferative effects and duration of JAK/STAT signaling activity. Cancer Biol Ther. 2008;7:1109.
pubmed: 18698163 doi: 10.4161/cbt.7.7.6192
McElrath C, Espinosa V, Lin JD, Peng J, Sridhar R, Dutta O, et al. Critical role of interferons in gastrointestinal injury repair. Nat Commun. 2021;12(1):2624.
pubmed: 33976143 pmcid: 8113246 doi: 10.1038/s41467-021-22928-0
Mahlakoiv T, Hernandez P, Gronke K, Diefenbach A, Staeheli P. Leukocyte-derived IFN-alpha/beta and epithelial IFN-lambda constitute a compartmentalized mucosal defense system that restricts enteric virus infections. PLoS Pathog. 2015. https://doi.org/10.1371/journal.ppat.1004782 .
doi: 10.1371/journal.ppat.1004782 pubmed: 25849543 pmcid: 4388470
Pott J. IFN-{lambda} determines the intestinal epithelial antiviral host defense. Proc Natl Acad Sci USA. 2011;108:7944.
pubmed: 21518880 pmcid: 3093475 doi: 10.1073/pnas.1100552108
Schneider WM, Chevillotte MD, Rice CM. Interferon-stimulated genes: a complex web of host defenses. Annu Rev Immunol. 2014;32:513–45.
pubmed: 24555472 pmcid: 4313732 doi: 10.1146/annurev-immunol-032713-120231
Wu J, Chen ZJ. Innate immune sensing and signaling of cytosolic nucleic acids. Annu Rev Immunol. 2014;32:461–88.
pubmed: 24655297 doi: 10.1146/annurev-immunol-032713-120156
Au WC, Moore PA, LaFleur DW, Tombal B, Pitha PM. Characterization of the interferon regulatory factor-7 and its potential role in the transcription activation of interferon A genes. J Biol Chem. 1998;273(44):29210–7.
pubmed: 9786932 doi: 10.1074/jbc.273.44.29210
Honda K, Yanai H, Negishi H, Asagiri M, Sato M, Mizutani T, et al. IRF-7 is the master regulator of type-I interferon-dependent immune responses. Nature. 2005;434(7034):772–7.
pubmed: 15800576 doi: 10.1038/nature03464
Senatus L, López-Díez R, Egaña-Gorroño L, Liu J, Hu J, Daffu G, et al. RAGE impairs murine diabetic atherosclerosis regression and implicates IRF7 in macrophage inflammation and cholesterol metabolism. JCI insight. 2020. https://doi.org/10.1172/jci.insight.137289 .
doi: 10.1172/jci.insight.137289 pubmed: 32641587 pmcid: 7406264
Minaga K, Watanabe T, Arai Y, Shiokawa M, Hara A, Yoshikawa T, et al. Activation of interferon regulatory factor 7 in plasmacytoid dendritic cells promotes experimental autoimmune pancreatitis. J Gastroenterol. 2020;55(5):565–76.
pubmed: 31960143 doi: 10.1007/s00535-020-01662-2
Kuroda M, Nishiguchi M, Ugawa N, Ishikawa E, Kawabata Y, Okamoto S, et al. Interferon regulatory factor 7 mediates obesity-associated MCP-1 transcription. PLoS ONE. 2020;15(5): e0233390.
pubmed: 32437400 pmcid: 7241760 doi: 10.1371/journal.pone.0233390
Li Y, Wang C, Wu X, Tian H, Jiang S, Xu T, et al. IRF3 and IRF7 contribute to diesel exhaust particles-induced pulmonary inflammation by mediating mTORC1 activation and restraining autophagy in mice. Eur J Immunol. 2020;50(8):1142–53.
pubmed: 32135578 doi: 10.1002/eji.201948415
Negishi H, Miki S, Sarashina H, Taguchi-Atarashi N, Nakajima A, Matsuki K, et al. Essential contribution of IRF3 to intestinal homeostasis and microbiota-mediated Tslp gene induction. Proc Natl Acad Sci USA. 2012;109(51):21016–21.
pubmed: 23213237 pmcid: 3529020 doi: 10.1073/pnas.1219482110
Zaki MH, Vogel P, Malireddi RK, Body-Malapel M, Anand PK, Bertin J, et al. The NOD-like receptor NLRP12 attenuates colon inflammation and tumorigenesis. Cancer Cell. 2011;20(5):649–60.
pubmed: 22094258 pmcid: 3761879 doi: 10.1016/j.ccr.2011.10.022
Gurung P, Malireddi RK, Anand PK, Demon D, Vande Walle L, Liu Z, et al. Toll or interleukin-1 receptor (TIR) domain-containing adaptor inducing interferon-beta (TRIF)-mediated caspase-11 protease production integrates toll-like receptor 4 (TLR4) protein- and Nlrp3 inflammasome-mediated host defense against enteropathogens. J Biol Chem. 2012;287(41):34474–83.
pubmed: 22898816 pmcid: 3464552 doi: 10.1074/jbc.M112.401406
Platanitis E, Decker T. Regulatory networks involving STATs, IRFs, and NFκB in inflammation. Front Immunol. 2018;9:2542.
pubmed: 30483250 pmcid: 6242948 doi: 10.3389/fimmu.2018.02542
Turpin W, Lee SH, Raygoza Garay JA, Madsen KL, Meddings JB, Bedrani L, et al. Increased intestinal permeability is associated with later development of Crohn’s disease. Gastroenterology. 2020;159(6):2092-100 e5.
pubmed: 32791132 doi: 10.1053/j.gastro.2020.08.005
Mehandru S, Colombel JF. The intestinal barrier, an arbitrator turned provocateur in IBD. Nat Rev Gastroenterol Hepatol. 2021;18(2):83–4.
pubmed: 33318680 doi: 10.1038/s41575-020-00399-w
Lopetuso LR, De Salvo C, Pastorelli L, Rana N, Senkfor HN, Petito V, et al. IL-33 promotes recovery from acute colitis by inducing miR-320 to stimulate epithelial restitution and repair. Proc Natl Acad Sci USA. 2018;115(40):E9362–70.
pubmed: 30224451 pmcid: 6176569 doi: 10.1073/pnas.1803613115
Li L, Zhou C, Li T, Xiao W, Yu M, Yang H. Interleukin-28A maintains the intestinal epithelial barrier function through regulation of claudin-1. Ann Transl Med. 2021;9(5):365.
pubmed: 33842586 pmcid: 8033364 doi: 10.21037/atm-20-5494
Lazear HM, Nice TJ, Diamond MS. Interferon-λ: immune functions at barrier surfaces and beyond. Immunity. 2015;43(1):15–28.
pubmed: 26200010 pmcid: 4527169 doi: 10.1016/j.immuni.2015.07.001
Ahn D, Wickersham M, Riquelme S, Prince A. The effects of IFN-λ on epithelial barrier function contribute to klebsiella pneumoniae ST258 pneumonia. Am J Respir Cell Mol Biol. 2019;60(2):158–66.
pubmed: 30183325 pmcid: 6376406 doi: 10.1165/rcmb.2018-0021OC
Antonczyk A, Krist B, Sajek M, Michalska A, Piaszyk-Borychowska A, Plens-Galaska M, et al. Direct inhibition of IRF-dependent transcriptional regulatory mechanisms associated with disease. Front Immunol. 2019;10:1176.
pubmed: 31178872 pmcid: 6543449 doi: 10.3389/fimmu.2019.01176
Neil JA, Matsuzawa-Ishimoto Y, Kernbauer-Hölzl E, Schuster SL, Sota S, Venzon M, et al. IFN-I and IL-22 mediate protective effects of intestinal viral infection. Nat Microbiol. 2019;4(10):1737–49.
pubmed: 31182797 pmcid: 6871771 doi: 10.1038/s41564-019-0470-1
Odendall C, Voak AA, Kagan JC. Type III IFNs are commonly induced by bacteria-sensing TLRs and reinforce epithelial barriers during infection. J Immunol. 2017;199(9):3270–9.
pubmed: 28954888 doi: 10.4049/jimmunol.1700250
Jefferies CA. Regulating IRFs in IFN driven disease. Front Immunol. 2019;10:325.
pubmed: 30984161 pmcid: 6449421 doi: 10.3389/fimmu.2019.00325
Read SA, Wijaya R, Ramezani-Moghadam M, Tay E, Schibeci S, Liddle C, et al. Macrophage coordination of the interferon lambda immune response. Front Immunol. 2019;10:2674.
pubmed: 31798594 pmcid: 6878940 doi: 10.3389/fimmu.2019.02674
Kim TH, Zhou H. Functional analysis of chicken IRF7 in response to dsRNA analog poly(I:C) by integrating overexpression and knockdown. PLoS ONE. 2015;10(7): e0133450.
pubmed: 26186542 pmcid: 4505898 doi: 10.1371/journal.pone.0133450
Windsor JW, Kaplan GG. Evolving epidemiology of IBD. Curr Gastroenterol Rep. 2019;21(8):40.
pubmed: 31338613 doi: 10.1007/s11894-019-0705-6
Ananthakrishnan AN, Bernstein CN, Iliopoulos D, Macpherson A, Neurath MF, Ali RAR, et al. Environmental triggers in IBD: a review of progress and evidence. Nat Rev Gastroenterol Hepatol. 2018;15(1):39–49.
pubmed: 29018271 doi: 10.1038/nrgastro.2017.136
Nambu R, Muise AM. Advanced understanding of monogenic inflammatory bowel disease. Front Pediatr. 2020;8: 618918.
pubmed: 33553075 doi: 10.3389/fped.2020.618918
Zhang X, Tong Y, Lyu X, Wang J, Wang Y, Yang R. Prevention and alleviation of dextran sulfate sodium salt-induced inflammatory bowel disease in mice with bacillus subtilis-fermented milk via inhibition of the inflammatory responses and regulation of the intestinal flora. Front Microbiol. 2020;11: 622354.
pubmed: 33519783 doi: 10.3389/fmicb.2020.622354
Banfi D, Moro E, Bosi A, Bistoletti M, Cerantola S, Crema F, et al. Impact of microbial metabolites on microbiota-gut-brain axis in inflammatory bowel disease. Int J Mol Sci. 2021;22(4):1623.
pubmed: 33562721 pmcid: 7915037 doi: 10.3390/ijms22041623
Deng Y, Guo SL, Li JQ, Xie SS, Zhou YC, Wei B, et al. Interferon regulatory factor 7 inhibits rat vascular smooth muscle cell proliferation and inflammation in monocrotaline-induced pulmonary hypertension. Life Sci. 2021;264: 118709.
pubmed: 33152351 doi: 10.1016/j.lfs.2020.118709
Chen Z, Gu Q, Chen R. Promotive role of IRF7 in ferroptosis of colonic epithelial cells in ulcerative colitis by the miR-375-3p/SLC11A2 axis. Biomol Biomed. 2023;23(3):437–49.
pubmed: 36336986 pmcid: 10171437
Spann KM, Loh Z, Lynch JP, Ullah A, Zhang V, Baturcam E, et al. IRF-3, IRF-7, and IPS-1 promote host defense against acute human metapneumovirus infection in neonatal mice. Am J Pathol. 2014;184(6):1795–806.
pubmed: 24726644 doi: 10.1016/j.ajpath.2014.02.026
del Fresno C, Soulat D, Roth S, Blazek K, Udalova I, Sancho D, et al. Interferon-beta production via Dectin-1-Syk-IRF5 signaling in dendritic cells is crucial for immunity to C. albicans. Immunity. 2013;38(6):1176–86.
pubmed: 23770228 doi: 10.1016/j.immuni.2013.05.010
Sin WX, Yeong JP, Lim TJF, Su IH, Connolly JE, Chin KC. IRF-7 mediates type I IFN responses in endotoxin-challenged mice. Front Immunol. 2020;11:640.
pubmed: 32373120 pmcid: 7176903 doi: 10.3389/fimmu.2020.00640
Bhalla N, Gardner CL, Downs SN, Dunn M, Sun C, Klimstra WB. Macromolecular synthesis shutoff resistance by myeloid cells is critical to IRF7-dependent systemic interferon alpha/beta induction after alphavirus infection. J Virol. 2019. https://doi.org/10.1128/JVI.00872-19 .
doi: 10.1128/JVI.00872-19 pubmed: 31578290 pmcid: 6880179
Tan Y, Guan Y, Sun Y, Zheng C. Correlation of intestinal mucosal healing and tight junction protein expression in ulcerative colitis patients. Am J Med Sci. 2019;357(3):195–204.
pubmed: 30638599 doi: 10.1016/j.amjms.2018.11.011
Yu M, Wang Q, Ma Y, Li L, Yu K, Zhang Z, et al. Aryl hydrocarbon receptor activation modulates intestinal epithelial barrier function by maintaining tight junction integrity. Int J Biol Sci. 2018;14(1):69–77.
pubmed: 29483826 pmcid: 5821050 doi: 10.7150/ijbs.22259
Li X, Li Q, Xiong B, Chen H, Wang X, Zhang D. Discoidin domain receptor 1(DDR1) promote intestinal barrier disruption in Ulcerative Colitis through tight junction proteins degradation and epithelium apoptosis. Pharmacol Res. 2022. https://doi.org/10.1016/j.phrs.2022.106368 .
doi: 10.1016/j.phrs.2022.106368 pubmed: 36586643 pmcid: 9712272
Samak G, Chaudhry Kamaljit K, Gangwar R, Narayanan D, Jaggar Jonathan H, Rao R. Calcium/Ask1/MKK7/JNK2/c-Src signalling cascade mediates disruption of intestinal epithelial tight junctions by dextran sulfate sodium. Biochemical Journal. 2015;465(3):503–15.
pubmed: 25377781 doi: 10.1042/BJ20140450
Zhang Z, Xue Z, Yang H, Zhao F, Liu C, Chen J, et al. Differential effects of EPA and DHA on DSS-induced colitis in mice and possible mechanisms involved. Food Funct. 2021;12(4):1803–17.
pubmed: 33523066 doi: 10.1039/D0FO02308F
Mahlakõiv T, Hernandez P, Gronke K, Diefenbach A, Staeheli P. Leukocyte-derived IFN-α/β and epithelial IFN-λ constitute a compartmentalized mucosal defense system that restricts enteric virus infections. PLoS Pathog. 2015;11(4): e1004782.
pubmed: 25849543 pmcid: 4388470 doi: 10.1371/journal.ppat.1004782
Blazek K, Eames HL, Weiss M, Byrne AJ, Perocheau D, Pease JE, et al. IFN-λ resolves inflammation via suppression of neutrophil infiltration and IL-1β production. J Exp Med. 2015;212(6):845–53.
pubmed: 25941255 pmcid: 4451128 doi: 10.1084/jem.20140995
Koltsida O, Hausding M, Stavropoulos A, Koch S, Tzelepis G, Ubel C, et al. IL-28A (IFN-λ2) modulates lung DC function to promote Th1 immune skewing and suppress allergic airway disease. EMBO Mol Med. 2011;3(6):348–61.
pubmed: 21538995 pmcid: 3377081 doi: 10.1002/emmm.201100142
Xu P, Becker H, Elizalde M, Pierik M, Masclee A, Jonkers D. Interleukin-28A induces epithelial barrier dysfunction in CD patient-derived intestinal organoids. Am J Physiol Gastrointest Liver Physiol. 2021;320(5):G689–99.
pubmed: 33595362 doi: 10.1152/ajpgi.00064.2020

Auteurs

Furong Qing (F)

School of Basic Medicine, Gannan Medical University, Ganzhou, Jiangxi, China.

Hongbo Tian (H)

Department of Stomatology, Chifeng Maternity Hospital, Chifeng, Inner Mongolia, China.

Biyao Wang (B)

Department of Gastroenterology, The Sixth-Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China.
Biomedical Innovation Center, The Sixth-Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China.

Bingyu Xie (B)

School of Basic Medicine, Gannan Medical University, Ganzhou, Jiangxi, China.

Lina Sui (L)

School of Basic Medicine, Gannan Medical University, Ganzhou, Jiangxi, China.

Xiaoyan Xie (X)

School of Basic Medicine, Gannan Medical University, Ganzhou, Jiangxi, China.

Wenji He (W)

School of Basic Medicine, Gannan Medical University, Ganzhou, Jiangxi, China.

Tiansheng He (T)

School of Basic Medicine, Gannan Medical University, Ganzhou, Jiangxi, China.

Yumei Li (Y)

School of Basic Medicine, Gannan Medical University, Ganzhou, Jiangxi, China.

Liangmei He (L)

Department of Gastroenterology, The First-Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, China.

Qin Guo (Q)

Department of Gastroenterology, The Sixth-Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China. guoq83@mail.sysu.edu.cn.
Biomedical Innovation Center, The Sixth-Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, China. guoq83@mail.sysu.edu.cn.

Zhiping Liu (Z)

School of Basic Medicine, Gannan Medical University, Ganzhou, Jiangxi, China. Zhiping.Liu@gmu.edu.cn.
Center for Immunology, Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases, Ministry of Education, Gannan Medical University, Ganzhou, Jiangxi, China. Zhiping.Liu@gmu.edu.cn.

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