Glycerophospholipid remodeling is critical for orthoflavivirus infection.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
07 Oct 2024
07 Oct 2024
Historique:
received:
21
02
2024
accepted:
26
09
2024
medline:
8
10
2024
pubmed:
8
10
2024
entrez:
7
10
2024
Statut:
epublish
Résumé
Flavivirus infection is tightly connected to host lipid metabolism. Here, we performed shotgun lipidomics of cells infected with neurotropic Zika, West Nile, and tick-borne encephalitis virus, as well as dengue and yellow fever virus. Early in infection specific lipids accumulate, e.g., neutral lipids in Zika and some lysophospholipids in all infections. Ceramide levels increase following infection with viruses that cause a cytopathic effect. In addition, fatty acid desaturation as well as glycerophospholipid metabolism are significantly altered. Importantly, depletion of enzymes involved in phosphatidylserine metabolism as well as phosphatidylinositol biosynthesis reduce orthoflavivirus titers and cytopathic effects while inhibition of fatty acid monounsaturation only rescues from virus-induced cell death. Interestingly, interfering with ceramide synthesis has opposing effects on virus replication and cytotoxicity depending on the targeted enzyme. Thus, lipid remodeling by orthoflaviviruses includes distinct changes but also common patterns shared by several viruses that are needed for efficient infection and replication.
Identifiants
pubmed: 39375358
doi: 10.1038/s41467-024-52979-y
pii: 10.1038/s41467-024-52979-y
doi:
Substances chimiques
Glycerophospholipids
0
Ceramides
0
Phosphatidylserines
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
8683Subventions
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : 517270163
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : 517270083
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : 197785619 (CRC 2021 project B10)
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : 416701689
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : 197785619 (CRC 2021 project Z02)
Informations de copyright
© 2024. The Author(s).
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