Immunotherapeutic potential of collagen V oral administration in mBSA/CFA-induced arthritis.
Animals
Male
Administration, Oral
Rats
Arthritis, Experimental
/ immunology
Rats, Inbred Lew
Synovial Membrane
/ immunology
Collagen Type V
/ immunology
Freund's Adjuvant
/ administration & dosage
Immunotherapy
/ methods
Interleukin-10
Forkhead Transcription Factors
/ metabolism
Serum Albumin, Bovine
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2024
2024
Historique:
received:
17
05
2024
accepted:
06
09
2024
medline:
8
10
2024
pubmed:
8
10
2024
entrez:
8
10
2024
Statut:
epublish
Résumé
We hypothesized that after synovial injury, collagen V (Col V) expose occult antigens, and Col V autoantibodies develop, indicating the loss of immune tolerance against this molecule, thus leading to damage to mesenchymal-derived cells as well as the extracellular matrix in experimental arthritis. Thus, the present study investigated the effects of oral administration of Col V on the synovium after the development of inflammation in mBSA/CFA-induced arthritis. After fourteen days of intraarticular administration of mBSA, 10 male Lewis rats were orally administered Col V (500 μg/300 μL) diluted in 0.01 N acetic acid (IA-Col V group). The arthritic group (IA group, n = 10) received only intraarticular mBSA. An intra-articular saline injection (20 μL) was given to the control group (CT-Col V, n = 5). IA group presented damaged synovia, the expansion of the extracellular matrix by cellular infiltrate, which was characterized by T and B lymphocytes, and fibroblastic infiltration. In contrast, after Col V oral immunotherapy IA-Col V group showed a significant reduction in synovial inflammation and intense expression of IL-10+ and FoxP3+ cells, in addition to a reduction in Col V and an increase in Col I in the synovia compared to those in the IA group. Furthermore, an increase in IL-10 production was detected after IA-Col V group spleen cell stimulation with Col V in vitro. PET imaging did not differ between the groups. The evaluation of oral treatment with Col V, after mBSA/CFA-induced arthritis in rats, protects against inflammation and reduces synovial tissue damage, through modulation of the synovial matrix, showing an immunotherapeutic potential in inhibiting synovitis.
Identifiants
pubmed: 39378196
doi: 10.1371/journal.pone.0311263
pii: PONE-D-24-19996
doi:
Substances chimiques
Collagen Type V
0
Freund's Adjuvant
9007-81-2
methylated bovine serum albumin
0
Interleukin-10
130068-27-8
Forkhead Transcription Factors
0
Foxp3 protein, rat
0
Serum Albumin, Bovine
27432CM55Q
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0311263Informations de copyright
Copyright: © 2024 Ramos da Silveira et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.