Replication kinetics and cytopathic effect of feline calicivirus in feline corneal epithelial cells.
cell culture
cornea
corneal epithelium
feline calicivirus
feline herpesvirus
ocular surface disease
Journal
Veterinary ophthalmology
ISSN: 1463-5224
Titre abrégé: Vet Ophthalmol
Pays: England
ID NLM: 100887377
Informations de publication
Date de publication:
08 Oct 2024
08 Oct 2024
Historique:
revised:
22
08
2024
received:
21
03
2024
accepted:
28
09
2024
medline:
9
10
2024
pubmed:
9
10
2024
entrez:
8
10
2024
Statut:
aheadofprint
Résumé
To determine the replication kinetics and cytopathic effect (CPE) of feline calicivirus (FCV) in feline corneal epithelial cells (FCEC). Seven archived FCV isolates and one archived feline herpesvirus type 1 (FHV-1) isolate, previously obtained from eight domestic short hair cats. FCV RNA was extracted for sequencing using Illumina MiSeq, to identify three genomically diverse isolates for further testing. Following reference-based assembly, viral genomes were annotated and assessed. Superficial keratectomies were performed to isolate the corneal epithelium of cats and the cells were cultured in vitro. FCEC were infected with the three chosen FCV isolates and one FHV-1 isolate at two different multiplicity of infection ratios (MOIs, 0.1 and 0.01 PFU/cell) and virus titration was assessed at 0, 2, 6, 12, 24, and 48 h post-infection (hpi). Viral identity was confirmed by RT-qPCR. Three genomically diverse FCV isolates were chosen for further assessment in the FCEC model. All infections of FCEC with FCV led to visible CPE, characterized by epithelial cell rounding and detachment from the plate by 24 hpi, while FHV-1 led to visible CPE within 48 hpi. All three of the FCV isolates replicated effectively in FCEC at both 0.1 and 0.01 MOI, with a peak increase in titer approximately 12-24 hpi. The results support the possible role of FCV as a primary pathogen of the feline ocular surface. FCV replicates in FCEC in vitro, leading to profound CPE.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Louisiana State University start-up funds
Organisme : Morris Animal Foundation
ID : D20FE-305
Informations de copyright
© 2024 American College of Veterinary Ophthalmologists.
Références
Spiri AM. An update on feline calicivirus. Schweiz Arch Tierheilkd. 2022;164(3):225‐241.
Radford AD, Coyne KP, Dawson S, Porter CJ, Gaskell RM. Feline calicivirus. Vet Res. 2007;38(2):319‐335.
Hofmann‐Lehmann R, Hosie MJ, Hartmann K, et al. Calicivirus infection in cats. Viruses. 2022;14(5):937.
Gerriets W, Joy N, Huebner‐Guthardt J, Eule JC. Feline calicivirus: a neglected cause of feline ocular surface infections? Vet Ophthalmol. 2012;15(3):172‐179.
Bordicchia M, Fumian TM, Van Brussel K, et al. Feline calicivirus virulent systemic disease: clinical epidemiology, analysis of viral isolates and in vitro efficacy of novel antivirals in Australian outbreaks. Viruses. 2021;13(10):2040.
Stiles J. Ocular manifestations of feline viral diseases. Vet J. 2014;201(2):166‐173.
Povey RC, Hale CJ. Experimental infections with feline caliciviruses (picornaviruses) in specific‐pathogen‐free kittens. J Comp Pathol. 1974;84(2):245‐256.
Lewin AC, Zhu X. Detection of mycoplasma spp. and feline calicivirus in cats with ocular surface disease. Vet Ophthalmol. 2024;27:382‐387.
Sosnovtsev SV, Prikhod'ko EA, Belliot G, Cohen JI, Green KY. Feline calicivirus replication induces apoptosis in cultured cells. Virus Res. 2003;94(1):1‐10.
Yang D‐K, Park Y‐R, Yoo JY, et al. Biological and molecular characterization of feline caliciviruses isolated from cats in South Korea. Korean J Vet Res. 2020;60(4):195‐202.
Lawson JS, Syme HM, Wheeler‐Jones CPD, Elliott J. Characterisation of crandell‐rees feline kidney (CRFK) cells as mesenchymal in phenotype. Res Vet Sci. 2019;127:99‐102.
Sandmeyer LS, Keller CB, Bienzle D. Culture of feline corneal epithelial cells and infection with feline herpesvirus‐1 as an investigative tool. Am J Vet Res. 2005;66(2):205‐209.
Mironovich MA, Yoon A, Marino ME, et al. Evaluation of compounded cidofovir, famciclovir, and ganciclovir for the treatment of feline herpesvirus ocular surface disease in shelter‐housed cats. Vet Ophthalmol. 2023;26(Suppl 1):143‐153.
Lewin AC, Kolb AW, McLellan GJ, et al. Genomic, recombinational and phylogenetic characterization of global feline herpesvirus 1 isolates. Virology. 2018;518:385‐397.
Marino ME, Mironovich MA, Ineck NE, et al. Full viral genome sequencing and phylogenomic analysis of feline herpesvirus type 1 (FHV‐1) in cheetahs (acinonyx jubatus). Viruses. 2021;13(11):2307.
Thieulent CJ, Carossino M, Peak L, Wolfson W, Balasuriya UBR. Development and validation of multiplex one‐step qPCR/RT‐qPCR assays for simultaneous detection of SARS‐CoV‐2 and pathogens associated with feline respiratory disease complex. PLoS One. 2024;19(3):e0297796.
Lewin AC, Coghill LM, Mironovich M, Liu CC, Carter RT, Ledbetter EC. Phylogenomic analysis of global isolates of canid Alphaherpesvirus 1. Viruses. 2020;12(12):1421.
Katoh K, Misawa K, Kuma K, Miyata T. MAFFT: a novel method for rapid multiple sequence alignment based on fast fourier transform. Nucleic Acids Res. 2002;30(14):3059‐3066.
Kalyaanamoorthy S, Minh BQ, Wong TKF, von Haeseler A, Jermiin LS. ModelFinder: fast model selection for accurate phylogenetic estimates. Nat Methods. 2017;14(6):587‐589.
Hartley C. Aetiology of corneal ulcers assume FHV‐1 unless proven otherwise. J Feline Med Surg. 2010;12(1):24‐35.
Maes R. Felid herpesvirus type 1 infection in cats: a natural host model for alphaherpesvirus pathogenesis. ISRN Vet Sci. 2012;2012:1‐14.
Gould D. Feline herpesvirus‐1: ocular manifestations, diagnosis and treatment options. J Feline Med Surg. 2011;13(5):333‐346.
Lewin AC, Hicks SK, Carter RT. A review of evidence‐based management of infectious ocular surface disease in shelter‐housed domestic cats. Vet Ophthalmol. 2023;26(Suppl 1):47‐58.
Bannasch MJ, Foley JE. Epidemiologic evaluation of multiple respiratory pathogens in cats in animal shelters. J Feline Med Surg. 2005;7(2):109‐119.
Zicola A, Saegerman C, Quatpers D, Viandier J, Thiry E. Feline herpesvirus 1 and feline calicivirus infections in a heterogeneous cat population of a rescue shelter. J Feline Med Surg. 2009;11(12):1023‐1027.
Van Der Meulen K, Garré B, Croubels S, Nauwynck H. In vitrocomparison of antiviral drugs against feline herpesvirus 1. BMC Vet Res. 2006;2(1):13.
Thomasy SM, Maggs DJ. A review of antiviral drugs and other compounds with activity against feline herpesvirus type 1. Vet Ophthalmol. 2016;19(Suppl 1):119‐130.
Stiles J. Feline ophthalmology. In: Gelatt KN, Gilger BC, Kern TJ, eds. Veterinary Ophthalmology. 5th ed. John Wiley and Sons; 2013:1477.
Hare D, Collins S, Cuddington B, Mossman K. The importance of physiologically relevant cell lines for studying virus–host interactions. Viruses. 2016;8(11):297.
Mori Y, Shimomura Y, Inoue Y, Kinoshita S. Susceptibility of cultured rabbit corneal epithelial cells to various herpes simplex virus isolates. Jpn J Ophthalmol. 1996;40(3):367‐370.
Geissler K, Schneider K, Platzer G, Truyen B, Kaaden OR, Truyen U. Genetic and antigenic heterogeneity among feline calicivirus isolates from distinct disease manifestations. Virus Res. 1997;48(2):193‐206.
Ossiboff RJ, Sheh A, Shotton J, Pesavento PA, Parker JSL. Feline caliciviruses (FCVs) isolated from cats with virulent systemic disease possess in vitro phenotypes distinct from those of other FCV isolates. J Gen Virol. 2007;88(Pt 2):506‐517.
Lee Y, Maes R, Tai SS, Soboll Hussey G. Viral replication and innate immunity of feline herpesvirus‐1 virulence‐associated genes in feline respiratory epithelial cells. Virus Res. Academic Press. 2019;264:56‐67.
Rampersad S, Tennant P. Chapter 3 ‐ Replication and Expression Strategies of Viruses. Viruses. Paula Tennant, Gustavo Fermin, Jerome E. Foster, (Eds.) 2018;55‐82. https://doi.org/10.1016/B978‐0‐12‐811257‐1.00003‐6
Payne S. Chapter 10 ‐ Introduction to RNA Viruses. Susan Payne, (Ed.) Viruses. Academic Press, 2017;97‐105. https://doi.org/10.1016/B978‐0‐12‐803109‐4.00010‐6
Cui Z, Li D, Xie Y, et al. Nitazoxanide protects cats from feline calicivirus infection and acts synergistically with mizoribine in vitro. Antivir Res. 2020;182:104827.
Fumian TM, Tuipulotu DE, Netzler NE, et al. Potential therapeutic agents for feline calicivirus infection. Viruses. 2018;10(8):433.