TRAF7 determines circadian period through ubiquitination and degradation of DBP.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
08 Oct 2024
08 Oct 2024
Historique:
received:
09
11
2023
accepted:
01
10
2024
medline:
9
10
2024
pubmed:
9
10
2024
entrez:
8
10
2024
Statut:
epublish
Résumé
D-site binding protein, DBP, is a clock-controlled transcription factor and drives daily rhythms of physiological processes through the regulation of an array of genes harboring a DNA binding motif, D-box. DBP protein levels show a circadian oscillation with an extremely robust peak/trough ratio, but it is elusive how the temporal pattern is regulated by post-translational regulation. In this study, we show that DBP protein levels are down-regulated by the ubiquitin-proteasome pathway. Analysis using 19 dominant-negative forms of E2 enzymes have revealed that UBE2G1 and UBE2T mediate the degradation of DBP. A proteomic analysis of DBP-interacting proteins and database screening have identified Tumor necrosis factor Receptor-Associated Factor 7 (TRAF7), a RING-type E3 ligase, that forms a complex with UBE2G1 and/or UBE2T. Ubiquitination analysis have revealed that TRAF7 enhances K48-linked polyubiquitination of DBP in cultured cells. Overexpression of TRAF7 down-regulates DBP protein level, while knockdown of TRAF7 up-regulates DBP in cultured cells. Knockout of TRAF7 in NIH3T3 cells have revealed that TRAF7 mediates the time-of-the-day-dependent regulation of DBP levels. Furthermore, TRAF7 has a period-shortening effect on the cellular clock. Together, TRAF7 plays an important role in circadian clock oscillation through destabilization of DBP.
Identifiants
pubmed: 39379486
doi: 10.1038/s42003-024-07002-x
pii: 10.1038/s42003-024-07002-x
doi:
Substances chimiques
DNA-Binding Proteins
0
Transcription Factors
0
Tumor Necrosis Factor Receptor-Associated Peptides and Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1280Subventions
Organisme : Ministry of Education, Culture, Sports, Science and Technology (MEXT)
ID : 24K09652, 17H06096, 24H02302, 21H05130, 21K18231
Organisme : Japan Agency for Medical Research and Development (AMED)
ID : 20gm6110010h0004, 22gm4010019h0001
Organisme : MEXT | Japan Science and Technology Agency (JST)
ID : JPMJFR2150
Informations de copyright
© 2024. The Author(s).
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