CREB3L1 deficiency impairs odontoblastic differentiation and molar dentin deposition partially through the TMEM30B.
Journal
International journal of oral science
ISSN: 2049-3169
Titre abrégé: Int J Oral Sci
Pays: India
ID NLM: 101504351
Informations de publication
Date de publication:
10 Oct 2024
10 Oct 2024
Historique:
received:
23
06
2024
accepted:
25
08
2024
revised:
22
08
2024
medline:
10
10
2024
pubmed:
10
10
2024
entrez:
9
10
2024
Statut:
epublish
Résumé
Odontoblasts are primarily responsible for synthesizing and secreting extracellular matrix proteins, which are crucial for dentinogenesis. Our previous single-cell profile and RNAscope for odontoblast lineage revealed that cyclic adenosine monophosphate responsive element-binding protein 3 like 1 (Creb3l1) was specifically enriched in the terminal differentiated odontoblasts. In this study, deletion of Creb3l1 in the Wnt1+ lineage led to insufficient root elongation and dentin deposition. Assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) and RNA sequencing were performed to revealed that in CREB3L1-deficient mouse dental papilla cells (mDPCs), the genes near the closed chromatin regions were mainly associated with mesenchymal development and the downregulated genes were primarily related to biological processes including cell differentiation, protein biosynthesis and transport, all of which were evidenced by a diminished ability of odontoblastic differentiation, a significant reduction in intracellular proteins, and an even greater decline in extracellular supernatant proteins. Dentin matrix protein 1 (Dmp1), dentin sialophosphoprotein (Dspp), and transmembrane protein 30B (Tmem30b) were identified as direct transcriptional regulatory targets. TMEM30B was intensively expressed in the differentiated odontoblasts, and exhibited a significant decline in both CREB3L1-deficient odontoblasts in vivo and in vitro. Deletion of Tmem30b impaired the ability of odontoblastic differentiation, protein synthesis, and protein secretion in mDPCs. Moreover, overexpressing TMEM30B in CREB3L1-deficient mDPCs partially rescued the extracellular proteins secretion. Collectively, our findings suggest that CREB3L1 participates in dentinogenesis and facilitates odontoblastic differentiation by directly enhancing the transcription of Dmp1, Dspp, and other differentiation-related genes and indirectly promoting protein secretion partially via TMEM30B.
Identifiants
pubmed: 39384739
doi: 10.1038/s41368-024-00322-y
pii: 10.1038/s41368-024-00322-y
doi:
Substances chimiques
Extracellular Matrix Proteins
0
Cyclic AMP Response Element-Binding Protein
0
Sialoglycoproteins
0
dentin sialophosphoprotein
0
Phosphoproteins
0
Membrane Proteins
0
Dmp1 protein, mouse
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
59Subventions
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : No. 82230029
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : No. 82071110
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : No. 82322014
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : No. 82270948
Informations de copyright
© 2024. The Author(s).
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