Dual spatial host-bacterial gene expression in Mycobacterium abscessus respiratory infections.
Mycobacterium abscessus
/ genetics
Animals
Mycobacterium Infections, Nontuberculous
/ microbiology
Mice
Host-Pathogen Interactions
/ genetics
Gene Expression Regulation, Bacterial
Respiratory Tract Infections
/ microbiology
Bacterial Proteins
/ genetics
Transcriptome
Lung
/ microbiology
Gene Expression Profiling
/ methods
Female
Virulence Factors
/ genetics
DNA-Directed RNA Polymerases
/ metabolism
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
09 Oct 2024
09 Oct 2024
Historique:
received:
29
09
2023
accepted:
20
09
2024
medline:
10
10
2024
pubmed:
10
10
2024
entrez:
9
10
2024
Statut:
epublish
Résumé
Co-localization of spatial transcriptome information of host and pathogen can revolutionize our understanding of microbial pathogenesis. Here, we aimed to demonstrate that customized bacterial probes can be successfully used to identify host-pathogen interactions in formalin-fixed-paraffin-embedded (FFPE) tissues by probe-based spatial transcriptomics technology. We analyzed the spatial gene expression of bacterial transcripts with the host transcriptomic profile in murine lung tissue chronically infected with Mycobacterium abscessus embedded in agar beads. Customized mycobacterial probes were designed for the constitutively expressed rpoB gene (an RNA polymerase β subunit) and the virulence factor precursor lsr2, modulated by oxidative stress. We found a correlation between the rpoB expression, bacterial abundance in the airways, and an increased expression of lsr2 virulence factor in lung tissue with high oxidative stress. Overall, we demonstrate the potential of dual bacterial and host gene expression assay in FFPE tissues, paving the way for the simultaneous detection of host and bacterial transcriptomes in pathological tissues.
Identifiants
pubmed: 39384974
doi: 10.1038/s42003-024-06929-5
pii: 10.1038/s42003-024-06929-5
doi:
Substances chimiques
Bacterial Proteins
0
Virulence Factors
0
DNA-Directed RNA Polymerases
EC 2.7.7.6
RNA polymerase beta subunit
EC 2.7.7.6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1287Subventions
Organisme : Ministero della Salute (Ministry of Health, Italy)
ID : GR-2021-12374157
Informations de copyright
© 2024. The Author(s).
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