A High-Throughput Screening Platform for Engineering Poly(ethylene Terephthalate) Hydrolases.

The ability of enzymes to hydrolyze the ubiquitous polyester, poly(ethylene terephthalate) (PET), has enabled the potential for bioindustrial recycling of this waste plastic. To date, many of these PET hydrolases have been engineered for improved catalytic activity and stability, but current screening methods have limitations in screening large libraries, including under high-temperature conditions. Here, we developed a platform that can simultaneously interrogate PET hydrolase libraries of 10

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© 2024 The Authors. Published by American Chemical Society.

Déclaration de conflit d'intérêts

The authors declare the following competing financial interest(s): The authors declare competing financial interests. The platform, methods and enzyme variants are the subject of domestic and foreign patent applications by Los Alamos National Laboratories on behalf of the Department of Energy and Triad National Security, L.L.C.