Biochemical and structural insights into a 5' to 3' RNA ligase reveal a potential role in tRNA ligation.
C12orf29
RLIG1
RNA ligase
tRNA
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
15 Oct 2024
15 Oct 2024
Historique:
medline:
10
10
2024
pubmed:
10
10
2024
entrez:
10
10
2024
Statut:
ppublish
Résumé
ATP-grasp superfamily enzymes contain a hand-like ATP-binding fold and catalyze a variety of reactions using a similar catalytic mechanism. More than 30 protein families are categorized in this superfamily, and they are involved in a plethora of cellular processes and human diseases. Here, we identify C12orf29 (RLIG1) as an atypical ATP-grasp enzyme that ligates RNA. Human RLIG1 and its homologs autoadenylate on an active site Lys residue as part of a reaction intermediate that specifically ligates RNA halves containing a 5'-phosphate and a 3'-hydroxyl. RLIG1 binds tRNA in cells and can ligate tRNA within the anticodon loop in vitro. Transcriptomic analyses of
Identifiants
pubmed: 39388274
doi: 10.1073/pnas.2408249121
doi:
Substances chimiques
RNA, Transfer
9014-25-9
RNA Ligase (ATP)
EC 6.5.1.3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2408249121Subventions
Organisme : Foundation for the NIH (FNIH)
ID : DP2GM137419
Organisme : Foundation for the NIH (FNIH)
ID : R01CA282036
Organisme : Welch Foundation (The Welch Foundation)
ID : I-1911
Organisme : Welch Foundation (The Welch Foundation)
ID : I-2088
Organisme : Welch Foundation (The Welch Foundation)
ID : I-1961
Organisme : Cancer Prevention and Research Institute of Texas (CPRIT)
ID : RP220309
Organisme : HHMI (HHMI)
ID : N/A
Organisme : HHMI (HHMI)
ID : N/A
Organisme : HHMI (HHMI)
ID : Hanna Gray fellowship
Organisme : Cancer Prevention and Research Institute of Texas (CPRIT)
ID : RR150033
Organisme : NIGMS NIH HHS
ID : R35 GM136216
Pays : United States
Déclaration de conflit d'intérêts
Competing interests statement:The authors declare no competing interest.