Investigation of the cytotoxic effect of current dentine bonding agents on human dental pulp cells.
Humans
Dental Pulp
/ drug effects
Dentin-Bonding Agents
/ toxicity
Calcium Compounds
/ toxicity
Cell Survival
/ drug effects
Silicates
/ toxicity
Aluminum Compounds
/ toxicity
Drug Combinations
Oxides
/ toxicity
Resin Cements
/ toxicity
Materials Testing
Biocompatible Materials
/ toxicity
Cell Line
Coloring Agents
Cell Culture Techniques
Bisphenol A-Glycidyl Methacrylate
/ toxicity
Trypan Blue
Cells, Cultured
Bonding agents
Cell viability
Dental pulp
Stem cell
Journal
BMC oral health
ISSN: 1472-6831
Titre abrégé: BMC Oral Health
Pays: England
ID NLM: 101088684
Informations de publication
Date de publication:
10 Oct 2024
10 Oct 2024
Historique:
received:
21
05
2024
accepted:
30
09
2024
medline:
11
10
2024
pubmed:
11
10
2024
entrez:
10
10
2024
Statut:
epublish
Résumé
An ideal aesthetic restorative material should be attached to the tooth tissues by adhesion, have a smooth surface as possible, should not cause toxic reactions in the pulp and discoloration and microleakage. This study aims at comparatively assess the cytotoxicity of current adhesive systems on human dental pulp cells. The adequate density of human pulp cells was observed from the ready cell line. The passaging was performed and the 3rd passage cells were selected. Adhesive systems and MTA were used on the cultures. Trypan blue staining was conducted on the cells at the 1st, 2nd, 3rd days and a count of live and dead cells using a light microscope. The dead cells whose membrane integrity was impaired by staining with trypan blue and the viability rate was determined using live and dead cell numbers. Data analysis was performed using IBM SPSS Statistics 22. A significant difference in vialibity rates between adhesive systems was observed on the first day. No significant statistical differences were observed on the 2nd and 3rd days (p < 0.05). Futurabond M showed similar biocompatibility with MTA on human pulp cells and it can be applied in cavities with 1-1.5 mm hard tissue between pulp and dentine.
Sections du résumé
BACKGROUND
BACKGROUND
An ideal aesthetic restorative material should be attached to the tooth tissues by adhesion, have a smooth surface as possible, should not cause toxic reactions in the pulp and discoloration and microleakage. This study aims at comparatively assess the cytotoxicity of current adhesive systems on human dental pulp cells.
MATERIALS AND METHODS
METHODS
The adequate density of human pulp cells was observed from the ready cell line. The passaging was performed and the 3rd passage cells were selected. Adhesive systems and MTA were used on the cultures. Trypan blue staining was conducted on the cells at the 1st, 2nd, 3rd days and a count of live and dead cells using a light microscope. The dead cells whose membrane integrity was impaired by staining with trypan blue and the viability rate was determined using live and dead cell numbers. Data analysis was performed using IBM SPSS Statistics 22.
RESULTS
RESULTS
A significant difference in vialibity rates between adhesive systems was observed on the first day. No significant statistical differences were observed on the 2nd and 3rd days (p < 0.05).
CONCLUSION
CONCLUSIONS
Futurabond M showed similar biocompatibility with MTA on human pulp cells and it can be applied in cavities with 1-1.5 mm hard tissue between pulp and dentine.
Identifiants
pubmed: 39390415
doi: 10.1186/s12903-024-04985-1
pii: 10.1186/s12903-024-04985-1
doi:
Substances chimiques
Dentin-Bonding Agents
0
Calcium Compounds
0
Silicates
0
Aluminum Compounds
0
mineral trioxide aggregate
0
Drug Combinations
0
Oxides
0
Resin Cements
0
Biocompatible Materials
0
Coloring Agents
0
Bisphenol A-Glycidyl Methacrylate
454I75YXY0
Trypan Blue
I2ZWO3LS3M
Types de publication
Journal Article
Comparative Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
1207Subventions
Organisme : Istanbul Üniversitesi
ID : 32787
Organisme : Istanbul Üniversitesi
ID : 32787
Organisme : Istanbul Üniversitesi
ID : 32787
Organisme : Istanbul Üniversitesi
ID : 32787
Informations de copyright
© 2024. The Author(s).
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