Optimized intravital three-photon imaging of intact mouse tibia links plasma cell motility to functional states.

Cell biology Optical imaging Small animal imaging

Journal

iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038

Informations de publication

Date de publication:
18 Oct 2024
Historique:
received: 25 11 2023
revised: 29 06 2024
accepted: 16 09 2024
medline: 11 10 2024
pubmed: 11 10 2024
entrez: 11 10 2024
Statut: epublish

Résumé

Intravital deep bone marrow imaging is crucial to studying cellular dynamics and functions but remains challenging, and minimally invasive methods are needed. We employed a high pulse-energy 1650 nm laser to perform three-photon microscopy

Identifiants

DOI: 10.1016/j.isci.2024.110985 PMID: 39391739 PMC: PMC11466647
pubmed: 39391739
doi: 10.1016/j.isci.2024.110985
pii: S2589-0042(24)02210-7
pmc: PMC11466647
doi:

Types de publication

Journal Article

Langues

eng

Pagination

110985

Informations de copyright

© 2024 The Author(s).

Déclaration de conflit d'intérêts

S. Domingue and L. Wooldridge are with Thorlabs LASER Division CO, US. J. Heidelin and V. Andresen are with Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.

Auteurs

Asylkhan Rakhymzhan (A)

German Rheumatism Research Center - a Leibniz Institute, Biophysical Analytics, Berlin, Germany.
German Rheumatism Research Center - a Leibniz Institute, Immune Dynamics, Berlin, Germany.
Charité - Universitätsmedizin, Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Clinics for Rheumatology and Clinical Immunology, Berlin, Germany.

Alexander F Fiedler (AF)

German Rheumatism Research Center - a Leibniz Institute, Biophysical Analytics, Berlin, Germany.
Freie Universität Berlin, Dynamic and Functional in vivo Imaging, Berlin, Germany.

Robert Günther (R)

German Rheumatism Research Center - a Leibniz Institute, Biophysical Analytics, Berlin, Germany.
German Rheumatism Research Center - a Leibniz Institute, Immune Dynamics, Berlin, Germany.

Scott R Domingue (SR)

Thorlabs Inc., Lafayette, CO, USA.

Laura Wooldridge (L)

Thorlabs Inc., Lafayette, CO, USA.

Ruth Leben (R)

German Rheumatism Research Center - a Leibniz Institute, Biophysical Analytics, Berlin, Germany.
Freie Universität Berlin, Dynamic and Functional in vivo Imaging, Berlin, Germany.

Yu Cao (Y)

German Rheumatism Research Center - a Leibniz Institute, Immune Dynamics, Berlin, Germany.
Charité - Universitätsmedizin, Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Clinics for Rheumatology and Clinical Immunology, Berlin, Germany.

Anne Bias (A)

German Rheumatism Research Center - a Leibniz Institute, Biophysical Analytics, Berlin, Germany.
Berlin University of Applied Sciences and Technology, Berlin, Germany.

Jay Roodselaar (J)

German Rheumatism Research Center - a Leibniz Institute, Immune Dynamics, Berlin, Germany.
Charité - Universitätsmedizin, Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Clinics for Rheumatology and Clinical Immunology, Berlin, Germany.

Ralf Köhler (R)

German Rheumatism Research Center - a Leibniz Institute, Biophysical Analytics, Berlin, Germany.
German Rheumatism Research Center - a Leibniz Institute, Immune Dynamics, Berlin, Germany.

Carolin Ulbricht (C)

German Rheumatism Research Center - a Leibniz Institute, Immune Dynamics, Berlin, Germany.
Charité - Universitätsmedizin, Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Clinics for Rheumatology and Clinical Immunology, Berlin, Germany.

Judith Heidelin (J)

Miltenyi Biotec GmbH, Bielefeld site, Bielefeld, Germany.

Volker Andresen (V)

Miltenyi Biotec GmbH, Bielefeld site, Bielefeld, Germany.

Ingeborg Beckers (I)

Berlin University of Applied Sciences and Technology, Berlin, Germany.

Astrid Haibel (A)

Berlin University of Applied Sciences and Technology, Berlin, Germany.

Georg Duda (G)

Charité - Universitätsmedizin, Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Julius Wolff Institute, Berlin, Germany.

Anja E Hauser (AE)

German Rheumatism Research Center - a Leibniz Institute, Immune Dynamics, Berlin, Germany.
Charité - Universitätsmedizin, Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Clinics for Rheumatology and Clinical Immunology, Berlin, Germany.

Raluca A Niesner (RA)

German Rheumatism Research Center - a Leibniz Institute, Biophysical Analytics, Berlin, Germany.
Freie Universität Berlin, Dynamic and Functional in vivo Imaging, Berlin, Germany.

Classifications MeSH