The antidepressant effect of Komagataella pastoris KM 71H in maternal separation mice model mediated by the microbiota-gut-brain axis.

BACKGROUND

The intestinal microbiota plays a fundamental role in maintaining host health, especially during childhood, a critical period for its establishment. Early life stress can lead to shifts in gut microbiota composition, thus increasing the risk of major depressive disorder (MDD) in adulthood. The supplementation with probiotics restores intestinal permeability and the health of gut microbial communities, therefore being potential study targets for the treatment of MDD. In this sense, the yeast Komagataella pastoris was reported as a promising probiotic with antidepressant effect.

METHODS

Hence, the present study aims to investigate this effect in mice submitted to maternal separation (MS) 3h per day from PND2 to PND14. Adult mice and mothers were treated with K. pastoris KM71H (8log UFC.g

RESULTS

On behavioral tests, K. pastoris KM71H treatment reduced the immobility time in TST of adult mice and increased the grooming activity in splash test of adult mice and mothers induced by MS. The probiotic treatment restored plasma corticosterone levels and glucocorticoid receptor expression in hippocampi, alongside nitrate/nitrite levels and superoxide dismutase activity in intestine, in addition to reducing reactive species levels in both structures. Moreover, it also normalized the fecal pH and water content of feces.

CONCLUSION

Thus, we conclude that K. pastoris KM71H is a promising therapeutic strategy for the treatment of MDD.

Informations de copyright

Copyright © 2024. Published by Elsevier B.V.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors have no conflict of interest in this article.