Precise identification and tracking of HMGCR-reactive CD4+ T cells in the target tissue of patients with anti-HMGCR immune-mediated necrotising myopathy.

Autoimmunity Polymyositis T-Lymphocytes

Journal

Annals of the rheumatic diseases
ISSN: 1468-2060
Titre abrégé: Ann Rheum Dis
Pays: England
ID NLM: 0372355

Informations de publication

Date de publication:
11 Oct 2024
Historique:
received: 27 02 2024
accepted: 14 09 2024
medline: 12 10 2024
pubmed: 12 10 2024
entrez: 11 10 2024
Statut: aheadofprint

Résumé

Anti-3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR)-positive immune-mediated necrotising myopathy (IMNM) is characterised by the presence of IgG autoantibodies against HMGCR and a strong association with specific HLA-DR alleles. Although these findings implicate HMGCR-specific CD4 Peripheral blood mononuclear cells from patients with anti-HMGCR+IMNM (n=10) and dermatomyositis (DM; n=10) were stimulated with HMGCR protein and peptides identified using a natural antigen processing assay (NAPA; n=6). CD4 CD4 HMGCR-antigen-reactive CD4

Sections du résumé

BACKGROUND BACKGROUND
Anti-3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR)-positive immune-mediated necrotising myopathy (IMNM) is characterised by the presence of IgG autoantibodies against HMGCR and a strong association with specific HLA-DR alleles. Although these findings implicate HMGCR-specific CD4
METHODS METHODS
Peripheral blood mononuclear cells from patients with anti-HMGCR+IMNM (n=10) and dermatomyositis (DM; n=10) were stimulated with HMGCR protein and peptides identified using a natural antigen processing assay (NAPA; n=6). CD4
RESULTS RESULTS
CD4
CONCLUSION CONCLUSIONS
HMGCR-antigen-reactive CD4

Identifiants

pubmed: 39393845
pii: ard-2024-225732
doi: 10.1136/ard-2024-225732
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© Author(s) (or their employer(s)) 2024. No commercial re-use. See rights and permissions. Published by BMJ on behalf of EULAR.

Déclaration de conflit d'intérêts

Competing interests: LC-S, AR and ALM have a patent for the anti-HMGCR antibody assay. ALM does not receive any compensation for this. ED is currently a paid employee of AstraZeneca.

Auteurs

Eleni Tiniakou (E)

Division of Rheumatology, Johns Hopkins University, School of Medicine, Baltimore, MD, USA etiniak1@jhmi.edu.

Alex Girgis (A)

Division of Rheumatology, Johns Hopkins University, School of Medicine, Baltimore, MD, USA.
Department of Biomedical Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Tiara Siafei (T)

Division of Rheumatology, Johns Hopkins University, School of Medicine, Baltimore, MD, USA.

Jemima Albayda (J)

Division of Rheumatology, Johns Hopkins University, School of Medicine, Baltimore, MD, USA.

Brittany Adler (B)

Division of Rheumatology, Johns Hopkins University, School of Medicine, Baltimore, MD, USA.

Julie J Paik (JJ)

Division of Rheumatology, Johns Hopkins University, School of Medicine, Baltimore, MD, USA.

Christopher A Mecoli (CA)

Division of Rheumatology, Johns Hopkins University, School of Medicine, Baltimore, MD, USA.

Alison Rebman (A)

Division of Rheumatology, Johns Hopkins University, School of Medicine, Baltimore, MD, USA.

Mark J Soloski (MJ)

Division of Rheumatology, Johns Hopkins University, School of Medicine, Baltimore, MD, USA.

Lisa Christopher-Stine (L)

Division of Rheumatology, Johns Hopkins University, School of Medicine, Baltimore, MD, USA.

Kellie N Smith (KN)

Department of Oncology and the Bloomberg-Kimmel Institute for Cancer Immunotherapy and Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Antony Rosen (A)

Division of Rheumatology, Johns Hopkins University, School of Medicine, Baltimore, MD, USA.

Andrew Lee Mammen (AL)

Division of Rheumatology, Johns Hopkins University, School of Medicine, Baltimore, MD, USA.
NIAMS/NIH, Bethesda, Maryland, USA.
Division of Neurology, Johns Hopkins University, School of Medicine, Baltimore, MS, USA.

Erika Darrah (E)

Division of Rheumatology, Johns Hopkins University, School of Medicine, Baltimore, MD, USA.

Classifications MeSH