Interactions between Helcococcus kunzii and Staphylococcus aureus: How a commensal bacterium modulates the virulence and metabolism of a pathogen in a chronic wound in vitro model.
Staphylococcus aureus
/ pathogenicity
Virulence
Bacterial Proteins
/ metabolism
Proteomics
Staphylococcal Infections
/ microbiology
Quorum Sensing
Gene Expression Regulation, Bacterial
Humans
Symbiosis
Trans-Activators
/ metabolism
Microbial Interactions
Virulence Factors
/ genetics
Proteome
Cell Wall
/ metabolism
Helcococcus kunzii
Staphylococcus aureus
Bacterial interactions
Chronic wound
In vitro model
Proteomic analysis
Journal
BMC microbiology
ISSN: 1471-2180
Titre abrégé: BMC Microbiol
Pays: England
ID NLM: 100966981
Informations de publication
Date de publication:
11 Oct 2024
11 Oct 2024
Historique:
received:
17
05
2024
accepted:
13
09
2024
medline:
12
10
2024
pubmed:
12
10
2024
entrez:
11
10
2024
Statut:
epublish
Résumé
Staphylococcus aureus is the predominant pathogen isolated in diabetic foot infections. Recently, the skin commensal bacterium, Helcococcus kunzii, was found to modulate the virulence of this pathogen in an in vivo model. This study aims to elucidate the molecular mechanisms underlying the interaction between these two bacterial species, using a proteomic approach. Our results reveal that H. kunzii can coexist and proliferate alongside S. aureus in a Chronic Wound Media (CWM), thereby mimicking an in vitro chronic wound environment. We noted that the secreted proteome of H. kunzii induced a transcriptional effect on S. aureus virulence, resulting in a decrease in the expression level of agrA, a gene involved in quorum sensing. The observed effect could be ascribed to specific proteins secreted by H. kunzii including polysaccharide deacetylase, peptidoglycan DD-metalloendopeptidase, glyceraldehyde-3-phosphate dehydrogenase, trypsin-like peptidase, and an extracellular solute-binding protein. These proteins potentially interact with the agr system, influencing S. aureus virulence. Additionally, the virulence of S. aureus was notably affected by modifications in iron-related pathways and components of cell wall architecture in the presence of H. kunzii. Furthermore, the overall metabolism of S. aureus was reduced when cocultured with H. kunzii. Future research will focus on elucidating the role of these excreted factors in modulating virulence.
Sections du résumé
BACKGROUND
BACKGROUND
Staphylococcus aureus is the predominant pathogen isolated in diabetic foot infections. Recently, the skin commensal bacterium, Helcococcus kunzii, was found to modulate the virulence of this pathogen in an in vivo model. This study aims to elucidate the molecular mechanisms underlying the interaction between these two bacterial species, using a proteomic approach.
RESULTS
RESULTS
Our results reveal that H. kunzii can coexist and proliferate alongside S. aureus in a Chronic Wound Media (CWM), thereby mimicking an in vitro chronic wound environment. We noted that the secreted proteome of H. kunzii induced a transcriptional effect on S. aureus virulence, resulting in a decrease in the expression level of agrA, a gene involved in quorum sensing. The observed effect could be ascribed to specific proteins secreted by H. kunzii including polysaccharide deacetylase, peptidoglycan DD-metalloendopeptidase, glyceraldehyde-3-phosphate dehydrogenase, trypsin-like peptidase, and an extracellular solute-binding protein. These proteins potentially interact with the agr system, influencing S. aureus virulence. Additionally, the virulence of S. aureus was notably affected by modifications in iron-related pathways and components of cell wall architecture in the presence of H. kunzii. Furthermore, the overall metabolism of S. aureus was reduced when cocultured with H. kunzii.
CONCLUSION
CONCLUSIONS
Future research will focus on elucidating the role of these excreted factors in modulating virulence.
Identifiants
pubmed: 39394082
doi: 10.1186/s12866-024-03520-0
pii: 10.1186/s12866-024-03520-0
doi:
Substances chimiques
Bacterial Proteins
0
Trans-Activators
0
Virulence Factors
0
Proteome
0
Agr protein, Staphylococcus aureus
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
406Subventions
Organisme : CHU Nîmes
ID : thématique phare 1
Organisme : CHU Nîmes
ID : thématique phare 1
Organisme : CHU Nîmes
ID : thématique phare 1
Informations de copyright
© 2024. The Author(s).
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