Systems analysis of miR-199a/b-5p and multiple miR-199a/b-5p targets during chondrogenesis.
bioinformatics
chondrogenesis
computational biology
human
kinetic modelling
microRNA
mouse
osteoarthritis
systems biology
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
14 Oct 2024
14 Oct 2024
Historique:
medline:
14
10
2024
pubmed:
14
10
2024
entrez:
14
10
2024
Statut:
epublish
Résumé
Changes in chondrocyte gene expression can contribute to the development of osteoarthritis (OA), and so recognition of the regulative processes during chondrogenesis can lead to a better understanding of OA. microRNAs (miRNAs) are key regulators of gene expression in chondrocytes/OA, and we have used a combined experimental, bioinformatic, and systems biology approach to explore the multiple miRNA-mRNA interactions that regulate chondrogenesis. A longitudinal chondrogenesis bioinformatic analysis identified paralogues miR-199a-5p and miR-199b-5p as pro-chondrogenic regulators. Experimental work in human cells demonstrated alteration of miR-199a-5p or miR-199b-5p expression led to significant inverse modulation of key chondrogenic genes and extracellular matrix production. miR-199a/b-5p targets
Identifiants
pubmed: 39401064
doi: 10.7554/eLife.89701
pii: 89701
doi:
pii:
Substances chimiques
MicroRNAs
0
mirn199 microRNA, human
0
Banques de données
GEO
['GSE274379', 'GSE18394', 'GSE109503']
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Dunhill Medical Trust
ID : R476/0516
Organisme : Novo Nordisk Fonden
ID : NNF17OC0027812
Organisme : Versus Arthritis
ID : MR/P020941/1
Pays : United Kingdom
Organisme : Versus Arthritis
ID : MR/R502182/1
Pays : United Kingdom
Organisme : Versus Arthritis
ID : 22043
Pays : United Kingdom
Informations de copyright
© 2023, Patel, Barter et al.
Déclaration de conflit d'intérêts
KP, MB, JS, PC, CP, IC, DY, DS No competing interests declared