Machine learning-based prediction of DNA G-quadruplex folding topology with G4ShapePredictor.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
16 10 2024
Historique:
received: 24 04 2024
accepted: 30 09 2024
medline: 17 10 2024
pubmed: 17 10 2024
entrez: 16 10 2024
Statut: epublish

Résumé

Deoxyribonucleic acid (DNA) is able to form non-canonical four-stranded helical structures with diverse folding patterns known as G-quadruplexes (G4s). G4 topologies are classified based on their relative strand orientation following the 5' to 3' phosphate backbone polarity. Broadly, G4 topologies are either parallel (4+0), antiparallel (2+2), or hybrid (3+1). G4s play crucial roles in biological processes such as DNA repair, DNA replication, transcription and have thus emerged as biological targets in drug design. While computational models have been developed to predict G4 formation, there is currently no existing model capable of predicting G4 folding topology based on its nucleic acid sequence. Therefore, we introduce G4ShapePredictor (G4SP), an application featuring a collection of multi-classification machine learning models that are trained on a custom G4 dataset combining entries from existing literature and in-house circular dichroism experiments. G4ShapePredictor is designed to accurately predict G4 folding topologies in potassium (

Identifiants

pubmed: 39414858
doi: 10.1038/s41598-024-74826-2
pii: 10.1038/s41598-024-74826-2
doi:

Substances chimiques

DNA 9007-49-2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

24238

Subventions

Organisme : Singapore Ministry of Education Academic Research Fund Tier 1
ID : RG140/22
Organisme : Singapore Ministry of Education Academic Research Fund Tier 1
ID : RG140/22
Organisme : Singapore Ministry of Education Academic Research Fund Tier 1
ID : RG140/22
Organisme : Singapore Ministry of Education Academic Research Fund Tier 2
ID : MOE-T2EP50223-0014

Informations de copyright

© 2024. The Author(s).

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Auteurs

Donn Liew (D)

Division of Physics and Applied Physics, School of Physical and Mathematical Sciences, Nanyang Technological University, 637371, Singapore, Singapore.

Zi Way Lim (ZW)

Division of Physics and Applied Physics, School of Physical and Mathematical Sciences, Nanyang Technological University, 637371, Singapore, Singapore.

Ee Hou Yong (EH)

Division of Physics and Applied Physics, School of Physical and Mathematical Sciences, Nanyang Technological University, 637371, Singapore, Singapore. eehou@ntu.edu.sg.

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