Alternative sources of molybdenum for Methanococcus maripaludis and their implication for the evolution of molybdoenzymes.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
16 Oct 2024
16 Oct 2024
Historique:
received:
01
07
2024
accepted:
10
10
2024
medline:
17
10
2024
pubmed:
17
10
2024
entrez:
16
10
2024
Statut:
epublish
Résumé
Molybdoenzymes are essential in global nitrogen, carbon, and sulfur cycling. To date, the only known bioavailable source of molybdenum (Mo) is molybdate. However, in the sulfidic and anoxic (euxinic) habitats that predominate in modern subsurface environments and that were pervasive prior to Earth's widespread oxygenation, Mo occurs as soluble tetrathiomolybdate ion and molybdenite mineral that is not known to be bioavailable. This presents a paradox for how organisms obtain Mo to support molybdoenzymes in these environments. Here, we show that tetrathiomolybdate and molybdenite sustain the high Mo demand of a model anaerobic methanogen, Methanococcus maripaludis, grown via Mo-dependent formate dehydrogenase, formylmethanofuran dehydrogenase, and nitrogenase. Cells grown with tetrathiomolybdate and molybdenite have similar growth kinetics, Mo content, and transcript levels of proteins involved in Mo transport and cofactor biosynthesis when compared to those grown with molybdate, implying similar mechanisms of transport and cofactor biosynthesis. These results help to reconcile the paradox of how Mo is acquired in modern and ancient anaerobes and provide new insight into how molybdoenzymes could have evolved prior to Earth's oxygenation.
Identifiants
pubmed: 39414898
doi: 10.1038/s42003-024-07049-w
pii: 10.1038/s42003-024-07049-w
doi:
Substances chimiques
Molybdenum
81AH48963U
Formate Dehydrogenases
EC 1.17.1.9
tetrathiomolybdate
91U3TGV99T
Nitrogenase
EC 1.18.6.1
molybdate
14259-85-9
Metalloproteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1337Subventions
Organisme : U.S. Department of Energy (DOE)
ID : DE-SC0020246
Informations de copyright
© 2024. The Author(s).
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