In vitro determination of genotoxicity and cytotoxicity induced by stainless steel brackets with and without surface coating in cultures of oral mucosal cells.
Stainless Steel
/ toxicity
Orthodontic Brackets
Humans
Mouth Mucosa
/ cytology
Surface Properties
Coated Materials, Biocompatible
/ toxicity
Fibroblasts
/ drug effects
In Vitro Techniques
Cells, Cultured
Microscopy, Electron, Scanning
Corrosion
Nanoparticles
/ toxicity
Materials Testing
Dental Alloys
/ toxicity
Biocompatibility
Corrosion
Cytotoxicity
Genotoxicity
Oral mucosal cell
Orthodontic brackets
Journal
BMC oral health
ISSN: 1472-6831
Titre abrégé: BMC Oral Health
Pays: England
ID NLM: 101088684
Informations de publication
Date de publication:
16 Oct 2024
16 Oct 2024
Historique:
received:
31
07
2024
accepted:
27
09
2024
medline:
17
10
2024
pubmed:
17
10
2024
entrez:
16
10
2024
Statut:
epublish
Résumé
Orthodontics is a speciality of dentistry that uses a plethora of devices made from myriad materials to manage various malocclusions. Prolonged contact of orthodontic appliances with oral tissues can lead to cellular damage, highlighting the need for biocompatible materials to mitigate health risks. To analyze the genotoxicity and cytotoxicity produced by metal brackets and coated metallic brackets with polymeric and nanoparticle coatings in oral mucosal cells. The current study compares the toxicity of 3 different types of orthodontic brackets with control groups of oral mucosal cells. Each of the three treatment groups consisted of 10 samples of orthodontic brackets: stainless steel brackets(Group 1), nanoparticle-coated brackets(Group 2), and polymeric-coated brackets(Group 3) exposed to corrosion eluates employing an oral biomimicry model. Two types of oral mucosal cells- Human Gingival Fibroblasts and Buccal Epithelial Cells were used to study the cytotoxic and/or genotoxic effects of the elutes. Intergroup comparisons were conducted using one-way analysis of variance, while scanning electron microscopy evaluated surface characteristic. The interaction between metal ions and oral mucosal cells showed no statistically significant difference for toxicity assays between the three groups(p > 0.005). However, polymeric and nanoparticle-coated groups showed reduced cellular differentiation when compared with conventional stainless-steel brackets. This in-vitro study shows that polymeric or nanoparticle coating of conventional metal brackets aids in enhancing corrosion-resistant characteristics of orthodontic appliances and reduces the toxic oral environment created by metal release in the oral cavity.
Sections du résumé
BACKGROUND
BACKGROUND
Orthodontics is a speciality of dentistry that uses a plethora of devices made from myriad materials to manage various malocclusions. Prolonged contact of orthodontic appliances with oral tissues can lead to cellular damage, highlighting the need for biocompatible materials to mitigate health risks.
OBJECTIVES
OBJECTIVE
To analyze the genotoxicity and cytotoxicity produced by metal brackets and coated metallic brackets with polymeric and nanoparticle coatings in oral mucosal cells.
MATERIALS & METHODS
METHODS
The current study compares the toxicity of 3 different types of orthodontic brackets with control groups of oral mucosal cells. Each of the three treatment groups consisted of 10 samples of orthodontic brackets: stainless steel brackets(Group 1), nanoparticle-coated brackets(Group 2), and polymeric-coated brackets(Group 3) exposed to corrosion eluates employing an oral biomimicry model. Two types of oral mucosal cells- Human Gingival Fibroblasts and Buccal Epithelial Cells were used to study the cytotoxic and/or genotoxic effects of the elutes. Intergroup comparisons were conducted using one-way analysis of variance, while scanning electron microscopy evaluated surface characteristic.
RESULTS
RESULTS
The interaction between metal ions and oral mucosal cells showed no statistically significant difference for toxicity assays between the three groups(p > 0.005). However, polymeric and nanoparticle-coated groups showed reduced cellular differentiation when compared with conventional stainless-steel brackets.
CONCLUSION
CONCLUSIONS
This in-vitro study shows that polymeric or nanoparticle coating of conventional metal brackets aids in enhancing corrosion-resistant characteristics of orthodontic appliances and reduces the toxic oral environment created by metal release in the oral cavity.
Identifiants
pubmed: 39415190
doi: 10.1186/s12903-024-04976-2
pii: 10.1186/s12903-024-04976-2
doi:
Substances chimiques
Stainless Steel
12597-68-1
Coated Materials, Biocompatible
0
Dental Alloys
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1233Informations de copyright
© 2024. The Author(s).
Références
Toy E, Yuksel S, Ozturk F, Karatas OH, Yalcin M. Evaluation of the genotoxicity and cytotoxicity in the buccal epithelial cells of patients undergoing orthodontic treatment with three light-cured bonding composites by using micronucleus testing. Korean J Orthod. 2014;44(3):128–35.
doi: 10.4041/kjod.2014.44.3.128
pubmed: 24892026
pmcid: 4040360
Faccioni F, Franceschetti P, Cerpelloni M, Fracasso ME. In vivo study on metal release from fixed orthodontic appliances and DNA damage in oral mucosa cells. Am J Orthod Dentofac Orthop. 2003;124(6):687–93.
doi: 10.1016/j.ajodo.2003.09.010
Zinelis S, Annousaki O, Eliades T, Makou M. Elemental composition of brazing alloys in metallic orthodontic brackets. Angle Orthod. 2004;74(3):394–9.
pubmed: 15264653
Loyola-Rodríguez JP, Lastra-Corso I, García-Cortés JO, Loyola-Leyva A, Domínguez-Pérez RA, Avila-Arizmendi D, Contreras-Palma G, González-Calixto C. Vitro Determination of Genotoxicity InducbracketsaalloysAllocultureslturhuman gingivalnfibroblastsblasts. J Toxicol. 2020;2020:1467456.
pubmed: 33488703
pmcid: 7787846
Wołowiec P, Chojnacka K, Loster BW, Mikulewicz M. Do dietary habits Influence Trace Elements Release from fixed Orthodontic Appliances? Biol Trace Elem Res. 2017;180(2):214–22.
doi: 10.1007/s12011-017-1011-5
pubmed: 28396985
pmcid: 5662677
Fernández-Miñano E, Ortiz C, Vicente A, Calvo Guirado JL, Ortiz AJ. Metallic ion content and damage to the DNA in oral mucosa cells of children with fixed orthodontic appliances. Biometals. 2011;24(5):935.
doi: 10.1007/s10534-011-9448-z
pubmed: 21468621
Hafez HS, Selim EM, Kamel Eid FH, Tawfik WA, Al-Ashkar EA, Mostafa YA. Cytotoxicity, genotoxicity, and metal release in patients with fixed orthodontic appliances: a longitudinal in-vivo study. Am J Orthod Dentofac Orthop. 2011;140(3):298–308.
doi: 10.1016/j.ajodo.2010.05.025
Downarowicz P, Mikulewicz M. Trace metal ions release from fixed orthodontic appliances and DNA damage in oral mucosa cells by in vivo studies: a literature review. Adv Clin Exp Med. 2017;26(7):1155–62.
doi: 10.17219/acem/65726
pubmed: 29211366
Angelieri F, Marcondes JP, de Almeida DC, Salvadori DM, Ribeiro DA. Genotoxicity of corrosion eluates obtained from orthodontic brackets in vitro. Am J Orthod Dentofac Orthop. 2011;139(4):504–9.
doi: 10.1016/j.ajodo.2009.03.058
Fors R, Persson M. Nickel in dental plaque and saliva in patients with and without orthodontic appliances. Eur J Orthod. 2006;28(3):292–7.
doi: 10.1093/ejo/cji091
pubmed: 16415086
Menezes LM, Quintão CA, Bolognese AM. Urinary excretion levels of nickel in orthodontic patients. Am J Orthod Dentofac Orthop. 2007;131(5):635–8.
doi: 10.1016/j.ajodo.2005.07.022
Petoumenou E, Arndt M, Keilig L, Reimann S, Hoederath H, Eliades T, Jäger A, Bourauel C. Nickel concentration in the saliva of patients with nickel-titanium orthodontic appliances. Am J Orthod Dentofac Orthop. 2009;135(1):59–65.
doi: 10.1016/j.ajodo.2006.12.018
Singh DP, Sehgal V, Pradhan KL, Chandna A, Gupta R. Estimation of nickel and chromium in saliva of patients with fixed orthodontic appliances. World J Orthod. 2008 Fall;9(3):196–202.
Arndt M, Bruck A, Scully T, Jaeger A, Bourauel C. Nickel ion release from orthodontic NiTi wires under simulation of realistic in-situ conditions. J Mater Sci. 2005;40:3659–67.
doi: 10.1007/s10853-005-0448-7
Eliades T, Trapalis C, Eliades G, Katsavrias E. Salivary metal levels of orthodontic patients: a novel methodological and analytical approach. Eur J Orthod. 2003;25(1):103–6.
doi: 10.1093/ejo/25.1.103
pubmed: 12608730
Huang TH, Yen CC, Kao CT. Comparison of ion release from new and recycled orthodontic brackets. Am J Orthod Dentofac Orthop. 2001;120(1):68–75.
doi: 10.1067/mod.2001.113794
Sfondrini MF, Cacciafesta V, Maffia E, Massironi S, Scribante A, Alberti G, Biesuz R, Klersy C. Chromium release from new stainless steel, recycled and nickel-free orthodontic brackets. Angle Orthod. 2009;79(2):361–7.
doi: 10.2319/042108-223.1
pubmed: 19216607
Martín-Cameán A, Jos A, Cameán AM, Solano E, Iglesias-Linares A. Genotoxic and cytotoxic effects and gene expression changes induced by fixed orthodontic appliances in oral mucosa cells of patients: a systematic review. Toxicol Mech Methods. 2015;25(6):440–7.
doi: 10.3109/15376516.2015.1062951
pubmed: 26156198
Seiler HG, Sigel A, Sigel H. Handbook on Metals in Clinical and Analytical Chemistry. M. Dekker; 1994.
Kumar V, Cotran RS, Robbins SL. Robbins basic pathology. 7th ed. Saunders; 2003.
Hanawa T. Metal ion release from metal implants. Mater Sci Eng: C. 2004;24(6–8):745–52.
doi: 10.1016/j.msec.2004.08.018
Hwang CJ, Shin JS, Cha JY. Metal release from simulated fixed orthodontic appliances. Am J Orthod Dentofac Orthop. 2001;120(4):383–91.
doi: 10.1067/mod.2001.117911
Trombetta D, Mondello MR, Cimino F, Cristani M, Pergolizzi S, Saija A. Toxic effect of nickel in an in vitro model of human oral epithelium. Toxicol Lett. 2005;159(3):219–25.
doi: 10.1016/j.toxlet.2005.05.019
pubmed: 16011880
Lü X, Bao X, Huang Y, Qu Y, Lu H, Lu Z. Mechanisms of cytotoxicity of nickel ions based on gene expression profiles. Biomaterials. 2009;30(2):141–8.
doi: 10.1016/j.biomaterials.2008.09.011
pubmed: 18922574
Pereira BR, Tanaka OM, Lima AA, Guariza-Filho O, Maruo H, Camargo ES. Metal and ceramic bracket effects on human buccal mucosa epithelial cells. Angle Orthod. 2009;79(2):373–9.
doi: 10.2319/021508-92.1
pubmed: 19216594
Westphalen GH, Menezes LM, Prá D, Garcia GG, Schmitt VM, Henriques JA, Medina-Silva R. In vivo determination of genotoxicity induced by metals from orthodontic appliances using micronucleus and comet assays. Genet Mol Res. 2008;7(4):1259–66.
doi: 10.4238/vol7-4gmr508
pubmed: 19065761
Su L, Yu Y, Zhao Y, Liang F, Zhang X. Strong antibacterial polydopamine Coatings prepared by a shaking-assisted Method. Sci Rep. 2016;6:24420.
doi: 10.1038/srep24420
pubmed: 27080534
pmcid: 4832207
Wang Z, Zou Y, Li Y, Cheng Y. Metal-containing polydopamine nanomaterials: Catalysis, Energy, and Theranostics. Small. 2020;16(18):e1907042.
doi: 10.1002/smll.201907042
pubmed: 32220006
Rao M, Ashith MV, Suman E, Isloor AM, Shetty NJ, Natarajan S. Evaluation of the surface characteristics and antibacterial properties of Titanium dioxide nanotube and methacryloyloxyethylphosphorylcholine (MPC) coated orthodontic brackets-a comparative invitro study. Clin Oral Investig. 2024;28(6):323.
doi: 10.1007/s00784-024-05655-w
pubmed: 38761310
pmcid: 11102386
Newbury DE, Ritchie NW. Performing elemental microanalysis with high accuracy and high precision by scanning electron microscopy/silicon drift detector energy-dispersive X-ray spectrometry (SEM/SDD-EDS). J Mater Sci. 2015;50(2):493–518.
doi: 10.1007/s10853-014-8685-2
pubmed: 26346887
Visai L, De Nardo L, Punta C, Melone L, Cigada A, Imbriani M, Arciola CR. Titanium oxide antibacterial surfaces in biomedical devices. Int J Artif Organs. 2011;34(9):929–46.
doi: 10.5301/ijao.5000050
pubmed: 22094576
Andersson M, Agurell E, Vaghef H, Bolcsfoldi G, Hellman B. Extended-term cultures of human T-lymphocytes and the comet assay: a useful combination when testing for genotoxicity in vitro? Mutat Res. 2003;540(1):43–55.
doi: 10.1016/S1383-5718(03)00169-4
pubmed: 12972057
Laxmikanth SM, Malik SA, Ramachandra CS, Shetty S, Shetty A, Kumar A, Joseph R. WITHDRAWN: the cytotoxic effect of Titanium Oxide Surface modified Orthodontic Stainless Steel Wires. Iran J Orthod. 2019;14(1).
Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen. 2000;35(3):206–21.
doi: 10.1002/(SICI)1098-2280(2000)35:3<206::AID-EM8>3.0.CO;2-J
pubmed: 10737956
Braz MG, Marcondes JP, Matsumoto MA, Duarte MA, Salvadori DM, Ribeiro DA. Genotoxicity in primary human peripheral lymphocytes after exposure to radiopacifiers in vitro. J Mater Sci Mater Med. 2008;19(2):601–5.
doi: 10.1007/s10856-007-3000-2
pubmed: 17619988
Ladeira MS, Rodrigues MA, Salvadori DM, Queiroz DM, Freire-Maia DV. DNA damage in patients infected by Helicobacter pylori. Cancer Epidemiol Biomarkers Prev. 2004;13(4):631–7.
doi: 10.1158/1055-9965.631.13.4
pubmed: 15066929
Ladeira MS, Rodrigues MA, Freire-Maia DV, Salvadori DM. Use of Comet assay to assess DNA damage in patients infected by Helicobacter pylori: comparisons between visual and image analyses. Mutat Res. 2005;586(1):76–86.
doi: 10.1016/j.mrgentox.2005.06.003
pubmed: 16084756
Lee CH, Marekov LN, Kim S, Brahim JS, Park MH, Steinert PM. Small proline-rich protein 1 is the major component of the cell envelope of normal human oral keratinocytes. FEBS Lett. 2000;477(3):268–72.
doi: 10.1016/S0014-5793(00)01806-8
pubmed: 10908733
Pinhal D, Gontijo AM, Reyes VA, Salvadori DM. Viable human buccal mucosa cells do not yield typical nucleoids: impacts on the single-cell gel electrophoresis/Comet assay. Environ Mol Mutagen. 2006;47(2):117–26.
doi: 10.1002/em.20174
pubmed: 16258922
Tomakidi P, Koke U, Kern R, Erdinger L, Krüger H, Kohl A, Komposch G. Assessment of acute cyto- and genotoxicity of corrosion eluates obtained from orthodontic materials using monolayer cultures of immortalized human gingival keratinocytes. J Orofac Orthop. 2000;61(1):2–19.
doi: 10.1007/BF02340928
pubmed: 10682407
Eliades T, Pratsinis H, Kletsas D, Eliades G, Makou M. Characterization and cytotoxicity of ions released from stainless steel and nickel-titanium orthodontic alloys. Am J Orthod Dentofac Orthop. 2004;125(1):24–9.
doi: 10.1016/j.ajodo.2003.09.009
Matos de Souza R, Macedo de Menezes L. Nickel, chromium and iron levels in the saliva of patients with simulated fixed orthodontic appliances. Angle Orthod. 2008;78(2):345–50.
doi: 10.2319/111806-466.1
pubmed: 18251615
Haddad AC, Tortamano A, Souza AL, Oliveira PV. An in vitro comparison of nickel and chromium release from brackets. Braz Oral Res. 2009;23(4):399–406.
Amini F, Asadi E, Hakimpour D, Rakhshan A. Salivary nickel and chromium levels in Orthodontic patients with and without periodontitis: a preliminary historical cohort study. Biol Trace Elem Res. 2019;191(1):10–5.
doi: 10.1007/s12011-018-1582-9
pubmed: 30554383
Ortiz AJ, Fernández E, Vicente A, Calvo JL, Ortiz C. Metallic ions released from stainless steel, nickel-free, and titanium orthodontic alloys: toxicity and DNA damage. Am J Orthod Dentofac Orthop. 2011;140(3):e115–22.
doi: 10.1016/j.ajodo.2011.02.021
Buljan ZI, Ribaric SP, Abram M, Ivankovic A, Spalj S. In vitro oxidative stress induced by conventional and self-ligating brackets. Angle Orthod. 2012;82(2):340–5.
doi: 10.2319/061811-395.1
pubmed: 21913853
Kao CT, Ding SJ, Min Y, Hsu TC, Chou MY, Huang TH. The cytotoxicity of orthodontic metal bracket immersion media. Eur J Orthod. 2007;29(2):198–203.
doi: 10.1093/ejo/cjl083
pubmed: 17311801