CHUP1 restricts chloroplast movement and effector-triggered immunity in epidermal cells.
Chloroplast Unusual Positioning 1 (CHUP1)
chloroplast movement
effector‐triggered immunity (ETI)
epidermal chloroplast defense (ECD)
epidermal chloroplast response (ECR)
phototropin 2 (phot2)
reactive oxygen species
stromules
Journal
The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884
Informations de publication
Date de publication:
17 Oct 2024
17 Oct 2024
Historique:
received:
23
04
2024
accepted:
29
08
2024
medline:
17
10
2024
pubmed:
17
10
2024
entrez:
17
10
2024
Statut:
aheadofprint
Résumé
Chloroplast Unusual Positioning 1 (CHUP1) plays an important role in the chloroplast avoidance and accumulation responses in mesophyll cells. In epidermal cells, prior research showed silencing CHUP1-induced chloroplast stromules and amplified effector-triggered immunity (ETI); however, the underlying mechanisms remain largely unknown. CHUP1 has a dual function in anchoring chloroplasts and recruiting chloroplast-associated actin (cp-actin) filaments for blue light-induced movement. To determine which function is critical for ETI, we developed an approach to quantify chloroplast anchoring and movement in epidermal cells. Our data show that silencing NbCHUP1 in Nicotiana benthamiana plants increased epidermal chloroplast de-anchoring and basal movement but did not fully disrupt blue light-induced chloroplast movement. Silencing NbCHUP1 auto-activated epidermal chloroplast defense (ECD) responses including stromule formation, perinuclear chloroplast clustering, the epidermal chloroplast response (ECR), and the chloroplast reactive oxygen species (ROS), hydrogen peroxide (H
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM103446
Pays : United States
Organisme : NIGMS NIH HHS
ID : P20 GM139760
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM132582
Pays : United States
Organisme : NIGMS NIH HHS
ID : S10 OD016361
Pays : United States
Organisme : NIGMS NIH HHS
ID : S10 OD028725
Pays : United States
Organisme : NIGMS NIH HHS
ID : S10 OD030321
Pays : United States
Organisme : Division of Integrative Organismal Systems
ID : IOS 2054685
Informations de copyright
© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.
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