The phageome of patients with ulcerative colitis treated with donor fecal microbiota reveals markers associated with disease remission.
Humans
Colitis, Ulcerative
/ therapy
Fecal Microbiota Transplantation
Bacteriophages
/ genetics
Gastrointestinal Microbiome
/ genetics
Feces
/ microbiology
Double-Blind Method
Male
Female
Metagenomics
/ methods
Adult
Dysbiosis
/ microbiology
Middle Aged
Virome
/ genetics
Remission Induction
Anti-Bacterial Agents
/ therapeutic use
Biomarkers
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
17 Oct 2024
17 Oct 2024
Historique:
received:
16
06
2024
accepted:
08
10
2024
medline:
18
10
2024
pubmed:
18
10
2024
entrez:
17
10
2024
Statut:
epublish
Résumé
Bacteriophages are influential within the human gut microbiota, yet they remain understudied relative to bacteria. This is a limitation of studies on fecal microbiota transplantation (FMT) where bacteriophages likely influence outcome. Here, using metagenomics, we profile phage populations - the phageome - in individuals recruited into two double-blind randomized trials of FMT in ulcerative colitis. We leverage the trial designs to observe that phage populations behave similarly to bacterial populations, showing temporal stability in health, dysbiosis in active disease, modulation by antibiotic treatment and by FMT. We identify a donor bacteriophage putatively associated with disease remission, which on genomic analysis was found integrated in a bacterium classified to Oscillospiraceae, previously isolated from a centenarian and predicted to produce vitamin B complex except B12. Our study provides an in-depth assessment of phage populations during different states and suggests that bacteriophage tracking has utility in identifying determinants of disease activity and resolution.
Identifiants
pubmed: 39420033
doi: 10.1038/s41467-024-53454-4
pii: 10.1038/s41467-024-53454-4
doi:
Substances chimiques
Anti-Bacterial Agents
0
Biomarkers
0
Types de publication
Journal Article
Randomized Controlled Trial
Langues
eng
Sous-ensembles de citation
IM
Pagination
8979Subventions
Organisme : Crohn's and Colitis Foundation (Crohn's & Colitis Foundation)
ID : 988415
Organisme : Department of Health | National Health and Medical Research Council (NHMRC)
ID : APP2011047
Organisme : Department of Health | National Health and Medical Research Council (NHMRC)
ID : Investigator grant
Organisme : University of New South Wales (UNSW Australia)
ID : Scientia fellowship
Informations de copyright
© 2024. The Author(s).
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