Glucose metabolism controls monocyte homeostasis and migration but has no impact on atherosclerosis development in mice.

Animals Monocytes / metabolism Atherosclerosis / metabolism Glucose Transporter Type 1 / metabolism Cell Movement Homeostasis Glucose / metabolism Mice Receptors, CCR2 / metabolism Mice, Inbred C57BL Male Plaque, Atherosclerotic / metabolism Glycolysis Blood Glucose / metabolism Disease Models, Animal

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
19 Oct 2024
Historique:
received: 05 12 2023
accepted: 08 10 2024
medline: 19 10 2024
pubmed: 19 10 2024
entrez: 18 10 2024
Statut: epublish

Résumé

Monocytes directly contribute to atherosclerosis development by their recruitment to plaques in which they differentiate into macrophages. In the present study, we ask how modulating monocyte glucose metabolism could affect their homeostasis and their impact on atherosclerosis. Here we investigate how circulating metabolites control monocyte behavior in blood, bone marrow and peripheral tissues of mice. We find that serum glucose concentrations correlate with monocyte numbers. In diet-restricted mice, monocytes fail to metabolically reprogram from glycolysis to fatty acid oxidation, leading to reduced monocyte numbers in the blood. Mechanistically, Glut1-dependent glucose metabolism helps maintain CD115 membrane expression on monocytes and their progenitors, and regulates monocyte migratory capacity by modulating CCR2 expression. Results from genetic models and pharmacological inhibitors further depict the relative contribution of different metabolic pathways to the regulation of CD115 and CCR2 expression. Meanwhile, Glut1 inhibition does not impact atherosclerotic plaque development in mouse models despite dramatically reducing blood monocyte numbers, potentially due to the remaining monocytes having increased migratory capacity. Together, these data emphasize the role of glucose uptake and intracellular glucose metabolism in controlling monocyte homeostasis and functions.

Identifiants

DOI: 10.1038/s41467-024-53267-5 PMID: 39424804
pubmed: 39424804
doi: 10.1038/s41467-024-53267-5
pii: 10.1038/s41467-024-53267-5
doi:

Substances chimiques

Glucose Transporter Type 1 0
Glucose IY9XDZ35W2
Slc2a1 protein, mouse 0
Receptors, CCR2 0
Ccr2 protein, mouse 0
Blood Glucose 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

9027

Subventions

Organisme : Agence Nationale de la Recherche (French National Research Agency)
ID : ANR-17-CE14-0017-01
Organisme : Agence Nationale de la Recherche (French National Research Agency)
ID : ANR-15-IDEX-01
Organisme : Austrian Science Fund (Fonds zur Förderung der Wissenschaftlichen Forschung)
ID : I 4646
Organisme : Fondation pour la Recherche Médicale (Foundation for Medical Research in France)
ID : EQU202303016719

Informations de copyright

© 2024. The Author(s).

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Auteurs

Alexandre Gallerand (A)

Université Côte d'Azur, CNRS, LP2M, Nice, France. Alexandre.gallerand@univ-cotedazur.fr.
Université Côte d'Azur, INSERM, C3M, Nice, France. Alexandre.gallerand@univ-cotedazur.fr.
ORCID: 0000-0002-3127-1343

Bastien Dolfi (B)

Université Côte d'Azur, CNRS, LP2M, Nice, France.
Université Côte d'Azur, INSERM, C3M, Nice, France.

Marion I Stunault (MI)

Université Côte d'Azur, INSERM, C3M, Nice, France.
ORCID: 0000-0002-4298-5114

Zakariya Caillot (Z)

Université Côte d'Azur, CNRS, LP2M, Nice, France.

Alexia Castiglione (A)

Université Côte d'Azur, CNRS, LP2M, Nice, France.
Université Côte d'Azur, INSERM, C3M, Nice, France.

Axelle Strazzulla (A)

Université Côte d'Azur, CNRS, LP2M, Nice, France.

Chuqiao Chen (C)

Department of Laboratory Medicine, Medical University of Vienna, 1090, Vienna, Austria.

Gyu Seong Heo (GS)

Department of Radiology, Washington University School of Medicine, Saint Louis, MO, USA.

Hannah Luehmann (H)

Department of Radiology, Washington University School of Medicine, Saint Louis, MO, USA.
ORCID: 0000-0002-7516-6772

Flora Batoul (F)

Université Côte d'Azur, INSERM, C3M, Nice, France.
ORCID: 0009-0006-0732-2872

Nathalie Vaillant (N)

Université Côte d'Azur, INSERM, C3M, Nice, France.

Adélie Dumont (A)

Université Côte d'Azur, INSERM, C3M, Nice, France.

Thomas Pilot (T)

Université Bourgogne Franche-Comté, LNC UMR1231, F-21000, Dijon, France.

Johanna Merlin (J)

Université Côte d'Azur, INSERM, C3M, Nice, France.
ORCID: 0000-0002-8659-3948

Fairouz N Zair (FN)

Université Côte d'Azur, CNRS, LP2M, Nice, France.

Jerome Gilleron (J)

Université Côte d'Azur, INSERM, C3M, Nice, France.

Adeline Bertola (A)

Université Côte d'Azur, CNRS, LP2M, Nice, France.

Peter Carmeliet (P)

Laboratory of Angiogenesis and Vascular Metabolism, Center for Cancer Biology (CCB), VIB, Department of Oncology, Leuven Cancer Institute (LKI), KU Leuven, Leuven, 3000, Belgium.
ORCID: 0000-0001-7961-1821

Jesse W Williams (JW)

Center for Immunology, Department of Integrative Biology and Physiology, University of Minnesota Medical School, Minneapolis, MN, USA.
ORCID: 0000-0003-3815-0891

Rafael J Arguello (RJ)

Aix Marseille University, CNRS, INSERM, CIML, Centre d'Immunologie de Marseille-Luminy, Marseille, France.
ORCID: 0000-0001-9785-3883

David Masson (D)

Université Bourgogne Franche-Comté, LNC UMR1231, F-21000, Dijon, France.
ORCID: 0000-0003-1692-0699

David Dombrowicz (D)

Univ.Lille, INSERM, CHU Lille, Institut Pasteur de Lille, U1011-EGID, 59000, Lille, France.
ORCID: 0000-0002-0485-8923

Laurent Yvan-Charvet (L)

Université Côte d'Azur, INSERM, C3M, Nice, France.
ORCID: 0000-0002-7748-4942

Denis Doyen (D)

Université Côte d'Azur, CNRS, LP2M, Nice, France.
Médecine Intensive Réanimation, Hôpital Pasteur, CHU de Nice, Nice, France.

Arvand Haschemi (A)

Department of Laboratory Medicine, Medical University of Vienna, 1090, Vienna, Austria.

Yongjian Liu (Y)

Department of Radiology, Washington University School of Medicine, Saint Louis, MO, USA.
ORCID: 0000-0002-1118-1535

Rodolphe R Guinamard (RR)

Université Côte d'Azur, CNRS, LP2M, Nice, France.
Université Côte d'Azur, INSERM, C3M, Nice, France.

Stoyan Ivanov (S)

Université Côte d'Azur, CNRS, LP2M, Nice, France. Stoyan.IVANOV@univ-cotedazur.fr.
Université Côte d'Azur, INSERM, C3M, Nice, France. Stoyan.IVANOV@univ-cotedazur.fr.
ORCID: 0000-0002-0527-2297

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Glucose metabolism controls monocyte...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Phagocytes › Monocytes › Glucose metabolism controls monocyte...
questionsmedicales.fr › Maladies cardiovasculaires › Maladies vasculaires › Artériopathies oblitérantes › Artériosclérose › Athérosclérose › Glucose metabolism controls monocyte...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Protéines transporteurs de solutés › Transporteurs de glucose par diffusion facilitée › Transporteur de glucose de type 1 › Glucose metabolism controls monocyte...
questionsmedicales.fr › Phénomènes physiologiques › Mouvement cellulaire › Glucose metabolism controls monocyte...
questionsmedicales.fr › Phénomènes physiologiques › Homéostasie › Glucose metabolism controls monocyte...
questionsmedicales.fr › Glucides › Sucres › Oses › Hexose › Glucose › Glucose metabolism controls monocyte...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Glucose metabolism controls monocyte...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs immunologiques › Récepteurs aux cytokines › Récepteurs aux chimiokines › Récepteurs CCR › Récepteurs CCR2 › Glucose metabolism controls monocyte...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Lignées consanguines de souris › Souris de lignée C57BL › Glucose metabolism controls monocyte...
questionsmedicales.fr › États, signes et symptômes pathologiques › États anatomopathologiques › Plaque d'athérosclérose › Glucose metabolism controls monocyte...
questionsmedicales.fr › Métabolisme › Voies et réseaux métaboliques › Glycolyse › Glucose metabolism controls monocyte...
questionsmedicales.fr › Glucides › Sucres › Oses › Hexose › Glucose › Glycémie › Glucose metabolism controls monocyte...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles biologiques › Modèles animaux de maladie humaine › Glucose metabolism controls monocyte...