Heterogeneity and Genomic Plasticity of Acinetobacter baumannii and Acinetobacter nosocomialis Isolates Recovered from Clinical Samples in India.

Acinetobacter baumannii / genetics Acinetobacter Infections / microbiology India Humans Genome, Bacterial Anti-Bacterial Agents / pharmacology Acinetobacter / genetics Microbial Sensitivity Tests Virulence Factors / genetics Plasmids / genetics Carbapenems / pharmacology Drug Resistance, Multiple, Bacterial / genetics Genomics Drug Resistance, Bacterial / genetics

Journal

Current microbiology
ISSN: 1432-0991
Titre abrégé: Curr Microbiol
Pays: United States
ID NLM: 7808448

Informations de publication

Date de publication:
19 Oct 2024
Historique:
received: 22 06 2024
accepted: 07 10 2024
medline: 19 10 2024
pubmed: 19 10 2024
entrez: 19 10 2024
Statut: epublish

Résumé

Acinetobacter baumannii and Acinetobacter nosocomialis are the imperious pathogens in the intensive care units. We aimed to explore the genomic features of these pathogens to understand the factors influencing their plasticity. Using next-generation sequencing, two carbapenem-resistant A. baumannii (AbaBS-3, AbaETR-4) isolates and a pan-susceptible A. nosocomialis (AbaAS-5) isolate were characterised. All genomes exhibited 94% similarity with a degree of heterogeneity. AbaBS-3 and AbaETR-4 harboured antibiotic resistance gene (ARG) repertoire to most antibiotic classes. Carbapenem resistance was due to blaOXA-23 and blaOXA-66 besides the antibiotic efflux pumps. Diverse mobile genetic elements (MGE), insertion sequences (IS), prophages and virulence determinants with a plethora of stress response genes were identified in all three genomes. Class-1 integron in AbaETR-4, encoded genes that confer resistance to aminoglycosides, phenicol, sulfonamides and disinfectants. Substitutions in LpxACD and PmrCAB of AbaETR-4 confirmed the colistin resistance in vitro. Novel mutations in piuA, responsible for transporting cefiderocol, were found in AbaBS-3 and AbaETR-4. Plasmids carrying toxin-antitoxin systems, ARGs and ISs were present in these genomes. All three genomes harboured diverse protein secretion systems, virulence determinants related to immune evasion, adherence, biofilm formation and iron acquisition systems. AbaAS-5 exclusively harboured serine protease pkf, and CpaA substrate of type-II secretion system but lacked the acinetobactin-iron acquisition system. Our work delivers a holistic genome characterization of A. baumannii, coupled with a trailblazing attempt to study A. nosocomialis from India. The presence of ARGs and potential virulence factors interspersed with MGE is a cause for concern, depicting the dynamic adaptability mediated by genetic recombination.

Identifiants

DOI: 10.1007/s00284-024-03942-z PMID: 39425793
pubmed: 39425793
doi: 10.1007/s00284-024-03942-z
pii: 10.1007/s00284-024-03942-z
doi:

Substances chimiques

Anti-Bacterial Agents 0
Virulence Factors 0
Carbapenems 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

415

Informations de copyright

© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

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Auteurs

Manasa Tantry (M)

Department of Microbiology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.

Tushar Shaw (T)

Faculty of Life and Allied Health Sciences, Ramaiah University of Applied Sciences, Bangalore, 560054, India.

Shwethapriya Rao (S)

Department of Critical Care, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.

Chiranjay Mukhopadhyay (C)

Department of Microbiology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.
Center for Emerging and Tropical Diseases, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.
Center for Antimicrobial Resistance and Education, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.

Chaitanya Tellapragada (C)

Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institute, 14183, Stockholm, Sweden.

Vandana Kalwaje Eshwara (V)

Department of Microbiology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India. vandana.ke@manipal.edu.
Center for Antimicrobial Resistance and Education, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India. vandana.ke@manipal.edu.
ORCID: 0000-0001-7561-4435

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Classifications MeSH

questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Moraxellaceae › Acinetobacter › Acinetobacter baumannii › Heterogeneity and Genomic Plasticity of...
questionsmedicales.fr › Infection › Infections bactériennes et mycoses › Infections bactériennes › Infections bactériennes à Gram négatif › Infections à Moraxellaceae › Infections à Acinetobacter › Heterogeneity and Genomic Plasticity of...
questionsmedicales.fr › Régions géographiques › Asie › Asie de l'Ouest › Asie du Sud › Inde › Heterogeneity and Genomic Plasticity of...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Heterogeneity and Genomic Plasticity of...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome microbien › Génome bactérien › Heterogeneity and Genomic Plasticity of...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Anti-infectieux › Antibactériens › Heterogeneity and Genomic Plasticity of...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Moraxellaceae › Acinetobacter › Heterogeneity and Genomic Plasticity of...
questionsmedicales.fr › Techniques d'investigation › Évaluation préclinique de médicament › Tests de sensibilité microbienne › Heterogeneity and Genomic Plasticity of...
questionsmedicales.fr › Facteurs biologiques › Toxines biologiques › Facteurs de virulence › Heterogeneity and Genomic Plasticity of...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Plasmides › Heterogeneity and Genomic Plasticity of...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérobicycliques › bêta-Lactames › Carbapénèmes › Heterogeneity and Genomic Plasticity of...
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes pharmacologiques › Résistance aux substances › Multirésistance aux médicaments › Multirésistance bactérienne aux médicaments › Heterogeneity and Genomic Plasticity of...
questionsmedicales.fr › Disciplines des sciences naturelles › Disciplines des sciences biologiques › Biologie › Génétique › Génomique › Heterogeneity and Genomic Plasticity of...
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes pharmacologiques › Résistance aux substances › Résistance microbienne aux médicaments › Résistance bactérienne aux médicaments › Heterogeneity and Genomic Plasticity of...