The proliferation and viability of human periodontal ligament stem cells cultured on polymeric scaffolds can be improved by low-level laser irradiation.
Humans
Periodontal Ligament
/ cytology
Cell Proliferation
/ radiation effects
Tissue Scaffolds
/ chemistry
Stem Cells
/ radiation effects
Cell Survival
/ radiation effects
Polyesters
/ chemistry
Lasers, Semiconductor
/ therapeutic use
Cells, Cultured
Microscopy, Electron, Scanning
Cell Cycle
/ radiation effects
Tissue Engineering
/ methods
Low-Level Light Therapy
/ methods
Cell proliferation
Laser therapy
Scaffolds
Stem cells
Tissue engineering
Journal
Lasers in medical science
ISSN: 1435-604X
Titre abrégé: Lasers Med Sci
Pays: England
ID NLM: 8611515
Informations de publication
Date de publication:
21 Oct 2024
21 Oct 2024
Historique:
received:
19
07
2024
accepted:
10
10
2024
medline:
21
10
2024
pubmed:
21
10
2024
entrez:
20
10
2024
Statut:
epublish
Résumé
This study assessed the impact of low-level laser irradiation on the viability and proliferation of human periodontal ligament stem cells (hPDLSCs) cultivated on polylactic acid (PLA) scaffolds. hPDLSCs were obtained, characterized, and grown on the surface of PLA films produced via the solvent casting technique. The study involved two groups: the control group, which was not exposed to radiation, and the laser group, which was irradiated with a diode laser (InGaAIP) with a power of 30 mW, a wavelength of 660 nm, and a single dose of 1 J/cm² emitted continuously. Cell viability was assessed 24 and 48 hours after irradiation using the Alamar blue and Live/Dead assays. Flow cytometry was used to assess cell cycle events, and scanning electron microscopy (SEM) was used to evaluate the interaction between cells and the biomaterial. The results revealed a statistically significant increase in cell metabolic activity in the laser group compared with the control group at 24 hours (p <0.05) and 48 hours (p <0.001), as indicated by the Alamar blue assay. The Live/Dead assay also revealed a greater density of viable cells in the laser group. The cell cycle analysis revealed a significant increase in the number of cells in the proliferative phase (G2/M) in the laser group compared with the control group (p <0.001). The SEM images demonstrated that the irradiated group had a greater concentration of cells while still maintaining their cell shape and projections. This study demonstrated that photobiomodulation can increase the viability and proliferation of periodontal stem cells cultured on PLA scaffolds, suggesting the potential of this protocol for future studies on periodontal tissue engineering.
Identifiants
pubmed: 39428431
doi: 10.1007/s10103-024-04210-z
pii: 10.1007/s10103-024-04210-z
doi:
Substances chimiques
poly(lactide)
459TN2L5F5
Polyesters
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
261Subventions
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : Conselho Nacional de Desenvolvimento Científico e Tecnológico
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.
Références
Ashammakhi N, GhavamiNejad A, Tutar R, Fricker A, Roy I, Chatzistavrou X, Hoque Apu E, Nguyen KL, Ahsan T, Pountos I, Caterson EJ (2022) Highlights on advancing frontiers in tissue Engineering. Tissue Eng Part B Rev 28(3):633–664. https://doi.org/10.1089/ten.TEB.2021.0012
doi: 10.1089/ten.TEB.2021.0012
pubmed: 34210148
pmcid: 9242713
Bakhshandeh B, Zarrintaj P, Oftadeh MO, Keramati F, Fouladiha H, Sohrabi-Jahromi S, Ziraksaz Z (2017) Tissue engineering; strategies, tissues, and biomaterials. Biotechnol Genet Eng Rev 33(2):144–172. https://doi.org/10.1080/02648725.2018.1430464
doi: 10.1080/02648725.2018.1430464
pubmed: 29385962
Langer R, Vacanti J (2016) Advances in tissue engineering. J Pediatr Surg 51(1):8–12. https://doi.org/10.1016/j.jpedsurg.2015.10.022
doi: 10.1016/j.jpedsurg.2015.10.022
pubmed: 26711689
Perić Kačarević Ž, Rider P, Alkildani S, Retnasingh S, Pejakić M, Schnettler R, Gosau M, Smeets R, Jung O, Barbeck M (2020) An introduction to bone tissue engineering. Int J Artif Organs 43(2):69–86. https://doi.org/10.1177/0391398819876286
doi: 10.1177/0391398819876286
pubmed: 31544576
Quarto R, Giannoni P (2016) Bone tissue Engineering: Past-Present-Future. Methods Mol Biol 1416:21–33. https://doi.org/10.1007/978-1-4939-3584-0_2
doi: 10.1007/978-1-4939-3584-0_2
pubmed: 27236664
Dehghan-Toranposhti S, Bakhshi R, Alizadeh R, Bohlouli M (2024) Fabrication, characterization and evaluating properties of 3D printed PLA-Mn scaffolds. Sci Rep 14(1):16592. https://doi.org/10.1038/s41598-024-67478-9
doi: 10.1038/s41598-024-67478-9
pubmed: 39025973
pmcid: 11258323
Zhao J, Liu X, Pu X, Shen Z, Xu W, Yang J (2024) Preparation Method and Application of Porous Poly(lactic acid) membranes: a review. Polym (Basel) 16(13):1846. https://doi.org/10.3390/polym16131846
doi: 10.3390/polym16131846
Singhvi MS, Zinjarde SS, Gokhale DV (2019) Polylactic acid: synthesis and biomedical applications. J Appl Microbiol 127(6):1612–1626. https://doi.org/10.1111/jam.14290
doi: 10.1111/jam.14290
pubmed: 31021482
Miguita L, Mantesso A, Pannuti CM, Deboni MCZ (2017) Can stem cells enhance bone formation in the human edentulous alveolar ridge? A systematic review and meta-analysis. Cell Tissue Bank 18(2):217–228. https://doi.org/10.1007/s10561-017-9612-y
doi: 10.1007/s10561-017-9612-y
pubmed: 28233169
Chalisserry EP, Nam SY, Park SH, Anil S (2017) Therapeutic potential of dental stem cells. J Tissue Eng 8:2041731417702531. https://doi.org/10.1177/2041731417702531
doi: 10.1177/2041731417702531
pubmed: 28616151
pmcid: 5461911
Seo BM, Miura M, Gronthos S, Bartold PM, Batouli S, Brahim J, Young M, Robey PG, Wang CY, Shi S (2004) Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet 364(9429):149–155. https://doi.org/10.1016/S0140-6736(04)16627-0
doi: 10.1016/S0140-6736(04)16627-0
pubmed: 15246727
Iwata T, Yamato M, Tsuchioka H, Takagi R, Mukobata S, Washio K, Okano T, Ishikawa I (2009) Periodontal regeneration with multi-layered periodontal ligament-derived cell sheets in a canine model. Biomaterials 30(14):2716–2723. https://doi.org/10.1016/j.biomaterials.2009.01.032
doi: 10.1016/j.biomaterials.2009.01.032
pubmed: 19201461
Roato I, Masante B, Putame G, Massai D, Mussano F (2022) Challenges of Periodontal tissue Engineering: increasing Biomimicry through 3D Printing and Controlled Dynamic Environment. Nanomaterials (Basel) 12(21):3878. https://doi.org/10.3390/nano12213878
doi: 10.3390/nano12213878
pubmed: 36364654
Soares DM, Ginani F, Henriques ÁG, Barboza CA (2015) Effects of laser therapy on the proliferation of human periodontal ligament stem cells. Lasers Med Sci 30(3):1171–1174. https://doi.org/10.1007/s10103-013-1436-9
doi: 10.1007/s10103-013-1436-9
pubmed: 24013624
Naureen B, Haseeb ASMA, Basirun WJ, Muhamad F (2021) Recent advances in tissue engineering scaffolds based on polyurethane and modified polyurethane. Mater Sci Eng C Mater Biol Appl 118:111228. https://doi.org/10.1016/j.msec.2020.111228
doi: 10.1016/j.msec.2020.111228
pubmed: 33254956
Ponnaiyan D, Rughwani RR, Shetty G, Mahendra J (2024) The effect of adjunctive LASER application on periodontal ligament stem cells. Front Cell Dev Biol 11:1341628. https://doi.org/10.3389/fcell.2023.1341628
doi: 10.3389/fcell.2023.1341628
pubmed: 38283989
pmcid: 10811063
Marques MM, Diniz IM, de Cara SP, Pedroni AC, Abe GL, D’Almeida-Couto RS, Lima PL, Tedesco TK, Moreira MS (2016) Photobiomodulation of Dental Derived Mesenchymal Stem cells: a systematic review. Photomed Laser Surg 34(11):500–508. https://doi.org/10.1089/pho.2015.4038
doi: 10.1089/pho.2015.4038
pubmed: 27058214
Hsu SH, Fu KY, Huang TB, Tsai IJ, Yen CY (2010) The effect of laser preexposure on seeding endothelial cells to a biomaterial surface. Photomed Laser Surg 28(Suppl 2):37–42. https://doi.org/10.1089/pho.2009.2613
doi: 10.1089/pho.2009.2613
Renno AC, McDonnell PA, Crovace MC, Zanotto ED, Laakso L (2010) Effect of 830 nm laser phototherapy on osteoblasts grown in vitro on Biosilicate scaffolds. Photomed Laser Surg 28(1):131–133. https://doi.org/10.1089/pho.2009.2487
doi: 10.1089/pho.2009.2487
pubmed: 19814702
Pinto KN, Tim CR, Crovace MC, Matsumoto MA, Parizotto NA, Zanotto ED, Peitl O, Rennó AC (2013) Effects of biosilicate(
doi: 10.1089/pho.2012.3435
pubmed: 23741994
Fernandes KR, Magri AMP, Kido HW, Parisi JR, Assis L, Fernandes KPS, Mesquita-Ferrari RA, Martins VC, Plepis AM, Zanotto ED, Peitl O, Renno ACM (2017) Biosilicate/PLGA osteogenic effects modulated by laser therapy: in vitro and in vivo studies. J Photochem Photobiol B 173:258–265. https://doi.org/10.1016/j.jphotobiol.2017.06.002
doi: 10.1016/j.jphotobiol.2017.06.002
pubmed: 28603084
Choi K, Kang BJ, Kim H, Lee S, Bae S, Kweon OK, Kim WH (2013) Low-level laser therapy promotes the osteogenic potential of adipose-derived mesenchymal stem cells seeded on an acellular dermal matrix. J Biomed Mater Res B Appl Biomater 101(6):919–928. https://doi.org/10.1002/jbm.b.32897
doi: 10.1002/jbm.b.32897
pubmed: 23529895
Incerti Parenti S, Panseri S, Gracco A, Sandri M, Tampieri A, Alessandri Bonetti G (2013) Effect of low-level laser irradiation on osteoblast-like cells cultured on porous hydroxyapatite scaffolds. Ann Ist Super Sanita 49(3):255–260. https://doi.org/10.4415/ANN_13_03_04
doi: 10.4415/ANN_13_03_04
pubmed: 24071604
Sabino VG, Ginani F, da Silva TN, Cabral AA, Mota-Filho HG, Freire MCLC, de Souza Furtado P, Assumpção PWMC, Cabral LM, Moura CE, Rocha HAO, de Souza Picciani PH, Barboza CAG (2020) Laser therapy increases the proliferation of preosteoblastic MC3T3-E1 cells cultured on poly(lactic acid) films. J Tissue Eng Regen Med 14(12):1792–1803. https://doi.org/10.1002/term.3134
doi: 10.1002/term.3134
pubmed: 33010118
Abo El-Dahab MM, El Deen GN, Aly RM, Gheith M (2024) Infrared diode laser enhances human periodontal ligament stem cells behaviour on titanium dental implants. Sci Rep 14(1):4155. https://doi.org/10.1038/s41598-024-54585-w
doi: 10.1038/s41598-024-54585-w
pubmed: 38378776
pmcid: 10879096
Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop Dj HE (2006) Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 8(4):315–317. https://doi.org/10.1080/14653240600855905
doi: 10.1080/14653240600855905
pubmed: 16923606
Emara A, Shah R (2021) Recent update on craniofacial tissue engineering. J Tissue Eng 12:20417314211003735. https://doi.org/10.1177/20417314211003735
doi: 10.1177/20417314211003735
pubmed: 33959245
pmcid: 8060749
Zhu W, Liang M (2015) Periodontal ligament stem cells: current status, concerns, and future prospects. Stem Cells Int., 2015, 972313. https://doi.org/10.1155/2015/972313
Ding G, Liu Y, Wang W, Wei F, Liu D, Fan Z, An Y, Zhang C, Wang S (2010) Allogeneic periodontal ligament stem cell therapy for periodontitis in swine. Stem Cells 28(10):1829–1838. https://doi.org/10.1002/stem.512
doi: 10.1002/stem.512
pubmed: 20979138
Liu J, Zhao Z, Ruan J, Weir MD, Ma T, Ren K, Schneider A, Oates TW, Li A, Zhao L, Xu HHK (2020) Stem cells in the periodontal ligament differentiated into osteogenic, fibrogenic and cementogenic lineages for the regeneration of the periodontal complex. J Dent 92:103259. https://doi.org/10.1016/j.jdent.2019.103259
doi: 10.1016/j.jdent.2019.103259
pubmed: 31809792
Tomokiyo A, Wada N, Maeda H (2019) Periodontal Ligament Stem cells: regenerative potency in Periodontium. Stem Cells Dev 28(15):974–985. https://doi.org/10.1089/scd.2019.0031
doi: 10.1089/scd.2019.0031
pubmed: 31215350
Ginani F, Soares DM, Barreto MP, Barboza CA (2015) Effect of low-level laser therapy on mesenchymal stem cell proliferation: a systematic review. Lasers Med Sci 30(8):2189–2194. https://doi.org/10.1007/s10103-015-1730-9
doi: 10.1007/s10103-015-1730-9
pubmed: 25764448
Marques NCT, Neto NL, Prado MTO, Vitor LLR, Oliveira RC, Sakai VT, Santos CF, Machado MAAM, Oliveira TM (2017) Effects of PBM in different energy densities and irradiance on maintaining cell viability and proliferation of pulp fibroblasts from human primary teeth. Lasers Med Sci 32(7):1621–1628. https://doi.org/10.1007/s10103-017-2301-z
doi: 10.1007/s10103-017-2301-z
pubmed: 28801810
Mylona V, Anagnostaki E, Chiniforush N, Barikani H, Lynch E, Grootveld M (2024) Photobiomodulation effects on Periodontal Ligament Stem cells: a systematic review of in Vitro studies. Curr Stem Cell Res Ther 19(4):544–558. https://doi.org/10.2174/1574888X17666220527090321
doi: 10.2174/1574888X17666220527090321
pubmed: 35638280
De Freitas LF, Hamblin MR (2016) Proposed mechanisms of Photobiomodulation or Low-Level Light Therapy. IEEE J Sel Top Quantum Electron 22(3):7000417. https://doi.org/10.1109/JSTQE.2016.2561201
doi: 10.1109/JSTQE.2016.2561201
pubmed: 28070154
pmcid: 5215870
Bomfim FRCD, Sella VRG, Thomasini RL, Plapler H (2018) Influence of low-level laser irradiation on osteocalcin protein and gene expression in bone tissue. Acta Cir Bras 33(9):736–743. https://doi.org/10.1590/s0102-865020180090000001
doi: 10.1590/s0102-865020180090000001
pubmed: 30328905
Gholami L, Khorsandi K, Fekrazad R (2023) Effect of red and near-infrared irradiation on periodontal ligament stem cells: ROS generation and cell cycle analysis. J Biomol Struct Dyn 41(19):10051–10058. https://doi.org/10.1080/07391102.2022.2152869
doi: 10.1080/07391102.2022.2152869
pubmed: 36469733
Deng M, Gu Y, Liu Z, Qi Y, Ma GE, Kang N (2015) Endothelial Differentiation of Human Adipose-Derived Stem Cells on Polyglycolic Acid/Polylactic Acid Mesh. Stem Cells Int., 2015, 350718. https://doi.org/10.1155/2015/350718