Development of multiplex real-time PCR for simultaneous detection of common fungal pathogens in invasive mycoses.

Humans Multiplex Polymerase Chain Reaction / methods Invasive Fungal Infections / diagnosis Real-Time Polymerase Chain Reaction / methods Sensitivity and Specificity Immunocompromised Host Fungi / isolation & purification DNA, Fungal / genetics Mycoses / diagnosis
Fungal pathogens Invasive mycoses Multiplex real-time PCR Specificity

Journal

PeerJ
ISSN: 2167-8359
Titre abrégé: PeerJ
Pays: United States
ID NLM: 101603425

Informations de publication

Date de publication:
2024
Historique:
received: 27 05 2024
accepted: 15 09 2024
medline: 21 10 2024
pubmed: 21 10 2024
entrez: 21 10 2024
Statut: epublish

Résumé

Fungi are common opportunistic pathogens that pose a significant threat to immunocompromised patients, particularly when late detection occurs. In this study a multiplex real-time PCR has been developed for simultaneous detection of common fungal pathogens associated with invasive mycoses in a diagnostic setting. The specificity of the assay was rigorously tested on 40 types of organisms ( This multiplex real-time PCR assay holds promise for enhancing the timely and accurate diagnosis of invasive mycoses, particularly in immunocompromised patient populations.

Sections du résumé

Background UNASSIGNED
Fungi are common opportunistic pathogens that pose a significant threat to immunocompromised patients, particularly when late detection occurs.
Methods UNASSIGNED
In this study a multiplex real-time PCR has been developed for simultaneous detection of common fungal pathogens associated with invasive mycoses in a diagnostic setting.
Results UNASSIGNED
The specificity of the assay was rigorously tested on 40 types of organisms (
Conclusion UNASSIGNED
This multiplex real-time PCR assay holds promise for enhancing the timely and accurate diagnosis of invasive mycoses, particularly in immunocompromised patient populations.

Identifiants

DOI: 10.7717/peerj.18238 PMID: 39430554 PMC: PMC11491059
pubmed: 39430554
doi: 10.7717/peerj.18238
pii: 18238
pmc: PMC11491059
doi:

Substances chimiques

DNA, Fungal 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e18238

Informations de copyright

© 2024 Ismadi et al.

Déclaration de conflit d'intérêts

The authors declare that they have no competing interests.

Auteurs

Yasmin Khairani Muhammad Ismadi (YKM)

Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.
ORCID: 0000-0002-2059-821X

Suharni Mohamad (S)

School of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.

Azian Harun (A)

Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.
Hospital Universiti Sains Malaysia, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Development of multiplex real-time PCR for...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine multiplex › Development of multiplex real-time PCR for...
questionsmedicales.fr › Infection › Infections bactériennes et mycoses › Mycoses › Infections fongiques invasives › Development of multiplex real-time PCR for...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine en temps réel › Development of multiplex real-time PCR for...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Sensibilité et spécificité › Development of multiplex real-time PCR for...
questionsmedicales.fr › Phénomènes du système immunitaire › Sujet immunodéprimé › Development of multiplex real-time PCR for...
questionsmedicales.fr › Eucaryotes › Champignons › Development of multiplex real-time PCR for...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN fongique › Development of multiplex real-time PCR for...
questionsmedicales.fr › Infection › Infections bactériennes et mycoses › Mycoses › Development of multiplex real-time PCR for...