The Bor1 elevator transport cycle is subject to autoinhibition and activation.

Arabidopsis / metabolism Arabidopsis Proteins / metabolism Cryoelectron Microscopy Phosphorylation Boron / metabolism Anion Transport Proteins / metabolism Biological Transport Models, Molecular Borates / metabolism Antiporters

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
22 Oct 2024
Historique:
received: 22 05 2024
accepted: 11 10 2024
medline: 22 10 2024
pubmed: 22 10 2024
entrez: 21 10 2024
Statut: epublish

Résumé

Boron, essential for plant growth, necessitates precise regulation due to its potential toxicity. This regulation is achieved by borate transporters (BORs), which are homologous to the SLC4 family. The Arabidopsis thaliana Bor1 (AtBor1) transporter from clade I undergoes slow regulation through degradation and translational suppression, but its potential for fast regulation via direct activity modulation was unclear. Here, we combine cryo-electron microscopy, mutagenesis, and functional characterization to study AtBor1, revealing high-resolution structures of the dimer in one inactive and three active states. Our findings show that AtBor1 is regulated by two distinct mechanisms: an autoinhibitory domain at the carboxyl terminus obstructs the substrate pathway via conserved salt bridges, and phosphorylation of Thr410 allows interaction with a positively charged pocket at the cytosolic face, essential for borate transport. These results elucidate the molecular basis of AtBor1's activity regulation and highlight its role in fast boron level regulation in plants.

Identifiants

DOI: 10.1038/s41467-024-53411-1 PMID: 39433547
pubmed: 39433547
doi: 10.1038/s41467-024-53411-1
pii: 10.1038/s41467-024-53411-1
doi:

Substances chimiques

Arabidopsis Proteins 0
BOR1 protein, Arabidopsis 0
Boron N9E3X5056Q
Anion Transport Proteins 0
Borates 0
Antiporters 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

9090

Informations de copyright

© 2024. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.

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Auteurs

Yan Jiang (Y)

Laboratory of Membrane Proteins and Structural Biology, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA. yan.jiang@sydney.edu.au.
Transporter Biology Group, School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Sydney, New South Wales, Australia. yan.jiang@sydney.edu.au.
ORCID: 0000-0001-5277-804X

Jiansen Jiang (J)

Laboratory of Membrane Proteins and Structural Biology, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA. jiansen.jiang@nih.gov.
ORCID: 0000-0002-1692-7906

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Brassicaceae › Arabidopsis › The Bor1 elevator transport cycle is subject...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Protéines d'Arabidopsis › The Bor1 elevator transport cycle is subject...
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Cryomicroscopie électronique › The Bor1 elevator transport cycle is subject...
questionsmedicales.fr › Métabolisme › Phosphorylation › The Bor1 elevator transport cycle is subject...
questionsmedicales.fr › Produits chimiques inorganiques › Éléments › Métalloïdes › Bore › The Bor1 elevator transport cycle is subject...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Pompes ioniques › Transporteurs d'anions › The Bor1 elevator transport cycle is subject...
questionsmedicales.fr › Métabolisme › Transport biologique › The Bor1 elevator transport cycle is subject...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › The Bor1 elevator transport cycle is subject...
questionsmedicales.fr › Composés chimiques organiques › Composés du bore › Acides boriques › Borates › The Bor1 elevator transport cycle is subject...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Pompes ioniques › Antiports › The Bor1 elevator transport cycle is subject...