Vital Dye Uptake of YO-PRO-1 and DASPEI Depends Upon Mechanoelectrical Transduction Function in Zebrafish Hair Cells.
cdh23
myo7aa
Gadolinium(iii) chloride
Mechanoelectrical transduction
Vital dyes
Zebrafish (Danio rerio)
Journal
Journal of the Association for Research in Otolaryngology : JARO
ISSN: 1438-7573
Titre abrégé: J Assoc Res Otolaryngol
Pays: United States
ID NLM: 100892857
Informations de publication
Date de publication:
21 Oct 2024
21 Oct 2024
Historique:
received:
21
05
2024
accepted:
27
09
2024
medline:
22
10
2024
pubmed:
22
10
2024
entrez:
21
10
2024
Statut:
aheadofprint
Résumé
Vital dyes allow the visualization of cells in vivo without causing tissue damage, making them a useful tool for studying lateral line and inner ear hair cells in living zebrafish and other vertebrates. FM1-43, YO-PRO-1, and DASPEI are three vital dyes commonly used for hair cell visualization. While it has been established that FM1-43 enters hair cells of zebrafish and other organisms through the mechanoelectrical transduction (MET) channel, the mechanism of entry into hair cells for YO-PRO-1 and DASPEI has not been established despite widespread use. We hypothesize that YO-PRO-1 and DASPEI entry into zebrafish hair cells is MET channel uptake dependent similar to FM1-43. To test this hypothesis, we used both genetic and pharmacologic means to block MET channel function. Genetic based MET channel assays were conducted with two different mechanotransduction defective zebrafish lines, specifically the myo7aa Five-day post fertilization (5dpf) myo7aa These results indicate that YO-PRO-1 and DASPEI entry into zebrafish hair cells is MET channel dependent similar to FM1-43. This knowledge expands the repertoire of vital dyes that can be used to assess mechanotransduction and MET channel function in zebrafish and other vertebrate models of hair cell function.
Identifiants
pubmed: 39433714
doi: 10.1007/s10162-024-00967-w
pii: 10.1007/s10162-024-00967-w
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIDCD NIH HHS
ID : R21DC020317
Pays : United States
Informations de copyright
© 2024. The Author(s) under exclusive licence to Association for Research in Otolaryngology.
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