Kinetochore dynein is sufficient to biorient chromosomes and remodel the outer kinetochore.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
21 Oct 2024
21 Oct 2024
Historique:
received:
29
02
2024
accepted:
25
09
2024
medline:
22
10
2024
pubmed:
22
10
2024
entrez:
21
10
2024
Statut:
epublish
Résumé
Multiple microtubule-directed activities concentrate on mitotic chromosomes to ensure their faithful segregation. These include couplers and dynamics regulators localized at the kinetochore, the microtubule interface built on centromeric chromatin, as well as motor proteins recruited to kinetochores and chromatin. Here, we describe an in vivo approach in the C. elegans one-cell embryo in which removal of the major microtubule-directed activities on mitotic chromosomes is compared to the selective presence of individual activities. Our approach reveals that the kinetochore dynein module, comprised of cytoplasmic dynein and its kinetochore-specific adapters, is sufficient to biorient chromosomes; by contrast, this module is unable to support congression. In coordination with orientation, the dynein module directs removal of outermost kinetochore components, including dynein itself, independently of the other microtubule-directed activities and kinetochore-localized protein phosphatase 1. These observations indicate that the kinetochore dynein module is sufficient to biorient chromosomes and to direct remodeling of the outer kinetochore in a microtubule attachment state-sensitive manner.
Identifiants
pubmed: 39433738
doi: 10.1038/s41467-024-52964-5
pii: 10.1038/s41467-024-52964-5
doi:
Substances chimiques
Caenorhabditis elegans Proteins
0
Dyneins
EC 3.6.4.2
Protein Phosphatase 1
EC 3.1.3.16
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
9085Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM074215
Pays : United States
Organisme : Wellcome Trust
ID : 215925
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 208833
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 107022
Pays : United Kingdom
Informations de copyright
© 2024. The Author(s).
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